Detailed view for TcCLB.508799.110

Basic information

TDR Targets ID: 50141
Trypanosoma cruzi, protein kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8704 | Length (AA): 413 | MW (Da): 46859 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
11 409 1omw (A) 94 457 19.00 0 1 1.02 0.22
96 406 2c30 (A) 399 659 32.00 0 1 1.03 -0.67
216 338 1wak (A) 189 553 29.00 0.000018 0.73 0.68 -0.9
4 405 3v5w (A) 77 453 19.00 0 1 0.972466 0.45
8 405 4tnd (A) 88 448 22.00 0 1 1.03278 0.45
198 410 3com (A) 108 286 35.00 0 1 0.818339 -0.73
202 327 4jrn (A) 373 474 42.00 0.017 0.7 0.413685 0.51
215 405 2pml (X) 147 321 29.00 0.00000049 0.9 0.66407 0.08

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_145884)

Species Accession Gene Product
Leishmania braziliensis LbrM.11.0280   protein kinase, putative
Leishmania donovani LdBPK_110560.1   protein kinase, putative
Leishmania infantum LinJ.11.0560   protein kinase, putative
Leishmania major LmjF.11.0510   protein kinase, putative
Leishmania mexicana LmxM.11.0510   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.11.7600   protein kinase, putative
Trypanosoma brucei Tb927.11.6690   NEK family Serine/threonine-protein kinase, putative
Trypanosoma congolense TcIL3000_0_06980   protein kinase, putative
Trypanosoma congolense TcIL3000.11.7250   protein kinase, putative
Trypanosoma cruzi TcCLB.506773.100   protein kinase, putative
Trypanosoma cruzi TcCLB.508799.110   protein kinase, putative

Essentiality

TcCLB.508799.110 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.4530 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.4530 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.4530 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.4530 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Mitogen-activated protein kinase 8 411 aa 23.6% 352 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0027 1 0.5
0.0081 0.5 0.5
0.0016 0.5 0.5
0.0059 1 1
0.0012 0.5 0.5
0.0067 0.5 0.5
0.0091 1 0.5
0.0088 0.4477 0.5
0.0018 0.5 0.5
0.0059 1 1
0.0081 1 0.5
0.0022 0.5 0.5
0.0012 0.5 0.5
0.0093 0.8828 0
0.0029 0.5 0.5
0.0039 0.5 0.5
0.0037 1 0.5
0.0056 1 0.5
0.0026 0.5 0.5
0.0069 0.3067 1
0.0098 0.3242 0.3649
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0063 1 0.5
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0063 0.7244 0.2543
0.0007 0.5 0.5
0.0092 1 0.5
0.0039 0.5 0.5
0.0008 0.5 0.5
0.0011 1 0.5
0.0066 0.3101 0
0.0033 0.5 0.5
0.0061 0.6883 0.5304
0.0032 0.5 0.5
0.0042 0.5 0.5
0.0064 0.3377 0
0.0004 0.5 0.5
0.0003 0.5 0.5
0.0059 1 1
0.0036 0.5 0.5
0.0039 0.9485 0.5
0.0007 0.5 0.5
0.0032 0.5 0.5
0.0033 1 0.5
0.0062 0.6935 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

21 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.508799.110 (Trypanosoma cruzi), protein kinase, putative
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