Detailed view for TcCLB.511817.40

Basic information

TDR Targets ID: 51284
Trypanosoma cruzi, NADH-cytochrome b5 reductase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.1756 | Length (AA): 288 | MW (Da): 32096 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00175   Oxidoreductase NAD-binding domain
PF00970   Oxidoreductase FAD-binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0009055   electron carrier activity  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 287 2bpo (A) 371 687 13.00 0 1 0.86 0.5
33 271 1umk (A) 37 299 39.00 0 1 1.28 -1.18
1 272 1cqx (A) 118 394 17.00 0.00000000015 1 1.15184 0.22
5 59 4uvq (A) 1109 1166 42.00 0.2 0.12 0.413072 1.14
29 288 2eix (A) 41 281 40.00 0 1 1.40788 -1
33 268 5gv8 (A) 1009 1268 36.00 0.0000005 1 1.23384 -0.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130211)

Species Accession Gene Product
Arabidopsis thaliana AT5G20080   NADH-cytochrome b5 reductase-like protein
Candida albicans CaO19.3507   NADH-cytochrome b5 reductase
Candida albicans CaO19.11001   NADH-cytochrome b5 reductase
Leishmania braziliensis LbrM.22.0710   NADH-cytochrome b5 reductase, putative
Leishmania braziliensis LbrM.15.0050   cytochrome-b5 reductase, putative
Leishmania braziliensis LbrM.22.0720   NADH-cytochrome b5 reductase, putative
Leishmania donovani LdBPK_220590.1   NADH-cytochrome b5 reductase, putative
Leishmania donovani LdBPK_150050.1   cytochrome-b5 reductase, putative
Leishmania donovani LdBPK_220600.1   NADH-cytochrome b5 reductase, putative
Leishmania infantum LinJ.15.0050   cytochrome-b5 reductase, putative
Leishmania infantum LinJ.22.0590   NADH-cytochrome b5 reductase, putative
Leishmania infantum LinJ.22.0600   NADH-cytochrome b5 reductase, putative
Leishmania major LmjF.22.0770   NADH-cytochrome b5 reductase, putative
Leishmania major LmjF.22.0780   NADH-cytochrome b5 reductase, putative
Leishmania major LmjF.15.0050   cytochrome-b5 reductase, putative
Leishmania mexicana LmxM.22.0770   NADH-cytochrome b5 reductase, putative
Leishmania mexicana LmxM.22.0780   NADH-cytochrome b5 reductase, putative
Leishmania mexicana LmxM.15.0050   cytochrome-b5 reductase, putative
Oryza sativa 4327126   Os01g0174300
Saccharomyces cerevisiae YKL150W   Mcr1p
Trypanosoma brucei gambiense Tbg972.7.3080   NADH-cytochrome b5 reductase, putative
Trypanosoma brucei gambiense Tbg972.5.2000   NADH-cytochrome b5 reductase, putative
Trypanosoma brucei gambiense Tbg972.7.3070   NADH-cytochrome b5 reductase, putative
Trypanosoma brucei Tb927.7.2710   NADH-cytochrome b5 reductase, putative
Trypanosoma brucei Tb927.7.2700   NADH-cytochrome b5 reductase, putative
Trypanosoma brucei Tb927.5.1470   NADH-cytochrome b5 reductase, putative
Trypanosoma congolense TcIL3000_7_2050   NADH-cytochrome b5 reductase, putative
Trypanosoma congolense TcIL3000_0_51330   NADH-cytochrome b5 reductase, putative
Trypanosoma congolense TcIL3000_7_2060   NADH-cytochrome b5 reductase, putative
Trypanosoma congolense TcIL3000_0_27380   NADH-cytochrome b5 reductase, putative
Trypanosoma cruzi TcCLB.510819.4   NADH-cytochrome b5 reductase, putative
Trypanosoma cruzi TcCLB.511817.40   NADH-cytochrome b5 reductase, putative

Essentiality

TcCLB.511817.40 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.2710 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.2710 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.2710 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.2710 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.5.1470 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.1470 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.1470 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.1470 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.7.2700 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.2700 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.2700 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.2700 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.511817.40 (Trypanosoma cruzi), NADH-cytochrome b5 reductase, putative
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