Detailed view for TcCLB.503589.10

Basic information

TDR Targets ID: 51548
Trypanosoma cruzi, GDP-mannose pyrophosphorylase

Source Database / ID:  TriTrypDB  GeneDB

pI: 7.2222 | Length (AA): 383 | MW (Da): 42125 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00132   Bacterial transferase hexapeptide (six repeats)
PF00483   Nucleotidyl transferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016779   nucleotidyltransferase activity  
GO:0016740   transferase activity  
GO:0009058   biosynthetic process  

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 383 1yp2 (A) 17 450 20.00 0 1 1.16 -0.83
14 380 1hv9 (A) 6 374 21.00 0 1 1.21 -0.28
277 381 1xhd (A) 28 140 16.00 0.000000000069 0.84 0.56 -1.27
12 383 2v0h (A) 4 438 28.00 0.000000000089 1 0.926979 1.08
14 124 4y7u (A) 1 108 43.00 0 1 0.890517 -0.97
14 353 2ggo (A) 1 321 33.00 0 1 1.24143 -0.4
273 319 2qia (A) 1 48 34.00 0 1 0.673215 -1.59
334 376 2qia (A) 17 60 16.00 0.067 0.58 0.332472 -0.46

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127109)

Species Accession Gene Product
Arabidopsis thaliana AT3G55590   probable mannose-1-phosphate guanylyltransferase 2
Arabidopsis thaliana AT4G30570   Glucose-1-phosphate adenylyltransferase family protein
Arabidopsis thaliana AT2G39770   GDP-mannose pyrophosphorylase/ mannose-1-pyrophosphatase
Babesia bovis BBOV_II006730   mannose-1-phosphate guanyltransferase, putative
Brugia malayi Bm1_40495   GDP-mannose pyrophosphorylase B
Candida albicans CaO19.6190   GDP-mannose pyrophosphorylase similar to S. cerevisiae PSA1 (YDL055C)
Caenorhabditis elegans CELE_C42C1.5   Protein TAG-335
Cryptosporidium hominis Chro.20192   GDP-mannose pyrophosphorylase (4N40)
Cryptosporidium parvum cgd2_1770   mannose-1-phosphate guanylyltransferase
Dictyostelium discoideum DDB_G0287619   GTP:alpha-D-mannose-1-phosphate guanylyltransferase
Drosophila melanogaster Dmel_CG1129   CG1129 gene product from transcript CG1129-RE
Echinococcus granulosus EgrG_000980800   mannose 1 phosphate guanyltransferase beta
Echinococcus granulosus EgrG_001172900   mannose 1 phosphate guanyltransferase beta
Echinococcus multilocularis EmuJ_001172900   mannose 1 phosphate guanyltransferase beta
Echinococcus multilocularis EmuJ_000980800   mannose 1 phosphate guanyltransferase beta
Homo sapiens ENSG00000173540   GDP-mannose pyrophosphorylase B
Leishmania braziliensis LbrM.23.0120   GDP-mannose pyrophosphorylase
Leishmania donovani LdBPK_230120.1   GDP-mannose pyrophosphorylase
Leishmania infantum LinJ.23.0120   GDP-mannose pyrophosphorylase
Leishmania major LmjF.23.0110   GDP-mannose pyrophosphorylase
Leishmania mexicana LmxM.23.0110   mannose-1-phosphate guanyltransferase
Loa Loa (eye worm) LOAG_09793   hypothetical protein
Loa Loa (eye worm) LOAG_13550   GDP-mannose pyrophosphorylase B
Loa Loa (eye worm) LOAG_16056   hypothetical protein
Loa Loa (eye worm) LOAG_05391   GDP-mannose pyrophosphorylase B
Mycobacterium leprae ML0753   Probable D-alpha-D-mannose-1-phosphate guanylyltransferase ManB (D-ALPHA-D-HEPTOSE-1-PHOSPHATE GUANYLYLTRANSFERASE)
Mus musculus ENSMUSG00000070284   GDP-mannose pyrophosphorylase B
Mycobacterium tuberculosis Rv3264c   D-alpha-D-mannose-1-phosphate guanylyltransferase ManB (D-alpha-D-heptose-1-phosphate guanylyltransferase)
Mycobacterium ulcerans MUL_2610   d-alpha-D-mannose-1-phosphate guanylyltransferase ManB
Neospora caninum NCLIV_028400   Nucleotidyl transferase family protein, related
Oryza sativa 4332373   Os03g0268400
Oryza sativa 4327472   Os01g0847200
Oryza sativa 4345021   Os08g0237200
Plasmodium berghei PBANKA_1022300   mannose-1-phosphate guanyltransferase, putative
Plasmodium falciparum PF3D7_1420900   mannose-1-phosphate guanyltransferase, putative
Plasmodium knowlesi PKNH_1337100   mannose-1-phosphate guanyltransferase, putative
Plasmodium vivax PVX_085435   mannose-1-phosphate guanyltransferase, putative
Plasmodium yoelii PY05717   GDP-mannose pyrophosphorylase
Saccharomyces cerevisiae YDL055C   mannose-1-phosphate guanylyltransferase
Schistosoma japonicum Sjp_0006520   ko:K00971 mannose-1-phosphate guanylyltransferase [EC2.7.7.22], putative
Schistosoma japonicum Sjp_0091930   Mannose-1-phosphate guanyltransferase beta, putative
Schistosoma mansoni Smp_000510   gdp-mannose pyrophosphorylase b isoform 2
Schistosoma mansoni Smp_028730   glucosamine-1-phosphate N-acetyltransferase
Schmidtea mediterranea mk4.018074.00   Mannose-1-phosphate guanyltransferase beta
Schmidtea mediterranea mk4.004902.07   Mannose-1-phosphate guanyltransferase beta
Schmidtea mediterranea mk4.001359.06  
Schmidtea mediterranea mk4.001359.04  
Schmidtea mediterranea mk4.012886.00   Mannose-1-phosphate guanyltransferase
Schmidtea mediterranea mk4.073271.00   Mannose-1-phosphate guanyltransferase beta
Schmidtea mediterranea mk4.006750.01   Mannose-1-phosphate guanyltransferase
Schmidtea mediterranea mk4.001359.02   Mannose-1-phosphate guanyltransferase beta
Trypanosoma brucei gambiense Tbg972.8.1690   mannose-1-phosphate guanyltransferase, putative
Trypanosoma brucei Tb927.8.2050   GDP-mannose pyrophosphorylase
Trypanosoma cruzi TcCLB.510947.10   GDP-mannose pyrophosphorylase
Trypanosoma cruzi TcCLB.503589.10   GDP-mannose pyrophosphorylase
Toxoplasma gondii TGME49_257960   GDP-D-mannose pyrophosphorylase
Theileria parva TP02_0639   GDP-mannose pyrophosphorylase, putative
Trichomonas vaginalis TVAG_394720   sugar-1-phosphate guanyl transferase, putative
Trichomonas vaginalis TVAG_178210   glucose-1-phosphate thymidylyltransferase, putative

Essentiality

TcCLB.503589.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.2050 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.2050 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.2050 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.8.2050 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C42C1.5 Caenorhabditis elegans adult lethal wormbase
CELE_C42C1.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_C42C1.5 Caenorhabditis elegans larval arrest wormbase
CELE_C42C1.5 Caenorhabditis elegans larval lethal wormbase
CELE_C42C1.5 Caenorhabditis elegans sterile wormbase
YDL055C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1022300 Plasmodium berghei Slow plasmo
TGME49_257960 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcCLB.503589.10 (Trypanosoma cruzi), GDP-mannose pyrophosphorylase
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