Detailed view for TcCLB.507673.10

Basic information

TDR Targets ID: 51588
Trypanosoma cruzi, serine/threonine-protein phosphatase PP1, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.2403 | Length (AA): 159 | MW (Da): 18087 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00149   Calcineurin-like phosphoesterase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 157 1jk7 (A) 142 298 57.00 0 1 1.67 -0.41
1 157 5jpe (A) 307 463 48.00 0 1 1.56382 -0.04
1 158 1s70 (A) 142 299 58.00 0 1 1.66291 0

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_152628)

Species Accession Gene Product
Leishmania braziliensis LbrM.20.0820   serine/threonine-protein phosphatase PP1, putative
Leishmania donovani LdBPK_340900.1   serine/threonine-protein phosphatase PP1, putative
Leishmania infantum LinJ.34.0900   serine/threonine-protein phosphatase PP1, putative
Leishmania major LmjF.34.0850   serine/threonine-protein phosphatase PP1, putative
Leishmania mexicana LmxM.33.0850   serine/threonine-protein phosphatase PP1, putative
Onchocerca volvulus OVOC3915  
Trypanosoma brucei gambiense Tbg972.4.3560   serine/threonine protein phosphatase PP1, putative
Trypanosoma brucei Tb927.4.3560   protein phoshatase 1, putative
Trypanosoma cruzi TcCLB.506201.30   serine/threonine-protein phosphatase PP1 beta
Trypanosoma cruzi TcCLB.507673.10   serine/threonine-protein phosphatase PP1, putative

Essentiality

TcCLB.507673.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.3560 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.3560 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.3560 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.3560 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased (PATO:0000468) epimastigote (BTO:0000409) inferred from in vitro culture assay (ECO:0000182) Trypanosoma cruzi 375443   376691   526768   529053  
Annotator: millerja@u.washington.edu. Comment: Association of these phenotype description with T. cruzi genes is based on amino acid sequences for 2 genes as shown in Figure 1 of referenced publication. References: 10678947
catalytic activity (GO:0003824) decreased (PATO:0000468) epimastigote (BTO:0000409) inferred from enzyme inhibition (ECO:0000184) Trypanosoma cruzi 375443   376691   526768   529053  
Annotator: millerja@u.washington.edu. Comment: Association of these phenotype description with T. cruzi genes is based on amino acid sequences for 2 genes as shown in Figure 1 of referenced publication. References: 10678947

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma cruzi serine/threonine-protein phosphatase PP1 beta Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0179 0.455 1
0.0179 0.455 1
0.0723 0.7783 1
0.0167 0.3794 0.5539
0.0751 0.7787 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier TcCLB.507673.10 (Trypanosoma cruzi), serine/threonine-protein phosphatase PP1, putative
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