Detailed view for TcCLB.420989.10

Basic information

TDR Targets ID: 52363
Trypanosoma cruzi, protein phosphatase 2C, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6924 | Length (AA): 207 | MW (Da): 22273 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00481   Protein phosphatase 2C

Gene Ontology

Mouse over links to read term descriptions.
GO:0004722   protein serine/threonine phosphatase activity  
GO:0003824   catalytic activity  
GO:0006470   protein amino acid dephosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 133 2isn (A) 210 334 41.00 0 1 0.905212 1.27
2 129 4n0g (A) 269 387 43.00 0.0000000037 1 0.831057 0.57
57 131 4oic (B) 375 457 31.00 0 1 0.772519 -0.64

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_141696)

Species Accession Gene Product
Arabidopsis thaliana AT5G53140   putative protein phosphatase 2C 76
Leishmania braziliensis LbrM.14.0870   protein phosphatase 2C-like protein
Leishmania donovani LdBPK_140960.1   protein phosphatase 2C-like protein
Leishmania infantum LinJ.14.0960   protein phosphatase 2C-like protein
Leishmania major LmjF.14.0900   protein phosphatase 2C-like protein
Leishmania mexicana LmxM.14.0900   protein phosphatase 2C-like protein
Oryza sativa 4339763   Os05g0587100
Oryza sativa 9269413   Os01g0618200
Trypanosoma brucei gambiense Tbg972.7.4510   protein phosphatase 2C, putative
Trypanosoma brucei Tb927.7.4020   protein phosphatase 2C, putative
Trypanosoma congolense TcIL3000_7_3230   protein phosphatase 2C, putative
Trypanosoma cruzi TcCLB.506659.39   protein phosphatase 2C, putative
Trypanosoma cruzi TcCLB.508865.4   protein phosphatase 2C, putative
Trypanosoma cruzi TcCLB.420989.10   protein phosphatase 2C, putative

Essentiality

TcCLB.420989.10 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.4020 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.4020 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.4020 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.4020 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Calcineurin (3.1.3.16 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

22 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.420989.10 (Trypanosoma cruzi), protein phosphatase 2C, putative
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