Detailed view for PF3D7_1227200

Basic information

TDR Targets ID: 5315
Plasmodium falciparum, potassium channel
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.7534 | Length (AA): 1966 | MW (Da): 232187 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 8

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07885   Ion channel

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0008076   voltage-gated potassium channel complex  
GO:0005249   voltage-gated potassium channel activity  
GO:0015269   calcium-activated potassium channel activity  
GO:0006813   potassium ion transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
704 801 1r3j (C) 25 122 18.00 0.0000045 0.1 0.45 -2.24
709 840 1lnq (A) 23 170 24.00 0.000029 0.18 0.23 -0.7
617 780 1orq (C) 44 222 28.00 0.0014 0.07 -0.0146819 2.31
701 784 4twk (B) 201 287 41.00 0.77 0.21 0.0736263 1.66
719 782 4h33 (A) 20 85 33.00 0.14 0.12 0.233453 -0.03
1379 1814 2vkh (A) 2 413 26.00 0.23 0.84 0.25167 0.8
212 543 2vse (A) 215 609 28.00 0.13 0.31 -0.0743292 2.1
615 778 1orq (C) 44 222 28.00 0.0015 0.07 -0.0147819 2.31
699 782 4twk (B) 201 287 41.00 0.78 0.21 0.0735263 1.66
1234 1422 4u5a (A) 48 239 17.00 0.12 0.23 0.187334 -0.18
1377 1730 2vkh (A) 2 358 24.00 0.083 1 0.200861 0.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, late ring, Ring, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, sporozoite, early ring, early schizont, early trophozoite, late schizont, late trophozoite, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_136898)

Species Accession Gene Product
Babesia bovis BBOV_II004900   conserved hypothetical protein
Cryptosporidium hominis Chro.60251   multi-pass transmembrane protein ion transporter
Cryptosporidium parvum cgd6_2140   conserved hypothetical multi-pass transmembrane protein, possible ion transporter
Neospora caninum NCLIV_034260   hypothetical protein, conserved
Plasmodium berghei PBANKA_1442000   potassium channel
Plasmodium falciparum PF3D7_1227200   potassium channel
Plasmodium knowlesi PKNH_1446600   potassium channel, putative
Plasmodium vivax PVX_123990   potassium channel, putative
Plasmodium yoelii PY05131   Ion transport protein, putative
Plasmodium yoelii PY02135   hypothetical protein
Toxoplasma gondii TGME49_273380   ion channel protein
Theileria parva TP02_0258   hypothetical protein

Essentiality

PF3D7_1227200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1442000 Plasmodium berghei Dispensable plasmo
TGME49_273380 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1227200 (Plasmodium falciparum), potassium channel
Title for this comment
Comment