Detailed view for TcCLB.504147.30

Basic information

TDR Targets ID: 53270
Trypanosoma cruzi, Protein of unknown function (DUF2870), putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.4868 | Length (AA): 265 | MW (Da): 29872 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF11069   Protein of unknown function (DUF2870)

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
120 194 3u7z (A) 57 128 32.00 0.73 0.95 0.469719 0.58
127 193 4gu2 (A) 1 70 21.00 0.00012 0.28 0.50553 -0.33
135 182 1bt0 (A) 12 59 17.00 0.86 0.06 0.341832 0.07
176 254 2c1i (A) 255 336 34.00 0.7 0.77 0.528813 0.36

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile epimastigote, metacyclic. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_130312)

Species Accession Gene Product
Drosophila melanogaster Dmel_CG18675   CG18675 gene product from transcript CG18675-RA
Echinococcus granulosus EgrG_001106800   EF HAND 1 calcium binding site
Echinococcus multilocularis EmuJ_001106800   EF HAND 1, calcium binding site
Giardia lamblia GL50803_15834   Hypothetical protein
Homo sapiens ENSG00000159079   chromosome 21 open reading frame 59
Leishmania braziliensis LbrM.35.4740   hypothetical protein, conserved
Leishmania donovani LdBPK_364720.1   Protein of unknown function (DUF2870), putative
Leishmania infantum LinJ.36.4720   hypothetical protein, conserved
Leishmania major LmjF.36.4500   hypothetical protein, conserved
Leishmania mexicana LmxM.36.4500   hypothetical protein, conserved
Mus musculus ENSMUSG00000022972   RIKEN cDNA 1110004E09 gene
Oryza sativa 4337648   Os05g0120800
Plasmodium berghei PBANKA_0824600   conserved protein, unknown function
Plasmodium falciparum PF3D7_0923700   conserved protein, unknown function
Plasmodium knowlesi PKNH_0721700   conserved protein, unknown function
Plasmodium vivax PVX_099595   conserved protein, unknown function
Plasmodium yoelii PY02396   hypothetical protein
Schistosoma japonicum Sjp_0023340   similar to Uncharacterized protein C21orf59, putative
Schistosoma mansoni Smp_074410   hypothetical protein
Schmidtea mediterranea mk4.000443.01   UPF0769 protein CG18675
Trypanosoma brucei gambiense Tbg972.10.12040   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.9870   Component of motile flagella 12
Trypanosoma congolense TcIL3000_10_8500   Protein of unknown function (DUF2870), putative
Trypanosoma cruzi TcCLB.504147.30   Protein of unknown function (DUF2870), putative
Toxoplasma gondii TGME49_269850   protein c21orf59, putative
Trichomonas vaginalis TVAG_417040   conserved hypothetical protein

Essentiality

TcCLB.504147.30 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9870 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.9870 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9870 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.9870 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0824600 Plasmodium berghei Dispensable plasmo
TGME49_269850 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tcru002412AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tcru002412; Recombinant protein: full-length; Source: T cruzi; protein c21orf59 ;

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TcCLB.504147.30 (Trypanosoma cruzi), Protein of unknown function (DUF2870), putative
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