Detailed view for PY02304

Basic information

TDR Targets ID: 536099
Plasmodium yoelii, cGMP-dependent protein kinase-related
Source Database / ID: 

pI: 5.5676 | Length (AA): 782 | MW (Da): 89347 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00027   Cyclic nucleotide-binding domain
PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004692   cGMP-dependent protein kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 758 5dyk (A) 86 853 92.00 0 1 2.01291 -0.65
369 426 2ptm (A) 566 624 41.00 0.00055 0.97 0.562069 -0.11
457 729 2y7j (A) 10 292 30.00 0 1 0.735705 -0.98

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127599)

Species Accession Gene Product
Arabidopsis thaliana AT2G20050   protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein
Babesia bovis BBOV_I004690   cGMP dependent protein kinase
Brugia malayi Bm1_37920   Egg laying defective protein 4
Caenorhabditis elegans CELE_F55A8.2   Protein EGL-4, isoform F
Caenorhabditis elegans CELE_C09G4.2   Protein PKG-2, isoform D
Cryptosporidium hominis Chro.80094   cGMP-dependent protein kinase
Cryptosporidium parvum cgd8_750   cyclic nucleotide (cGMP)-dependent protein kinase with 3 cNMP binding domains and a Ser/Thr kinase domain
Drosophila melanogaster Dmel_CG4839   CG4839 gene product from transcript CG4839-RA
Drosophila melanogaster Dmel_CG10033   foraging
Drosophila melanogaster Dmel_CG3324   cGMP-dependent protein kinase 21D
Echinococcus granulosus EgrG_000452500   cGMP dependent protein kinase 1
Echinococcus granulosus EgrG_000158500   cGMP dependent protein kinase
Echinococcus granulosus EgrG_000926500   cGMP dependent protein kinase 1
Echinococcus multilocularis EmuJ_000452500   cGMP dependent protein kinase 1
Echinococcus multilocularis EmuJ_000926500   cGMP dependent protein kinase 1
Echinococcus multilocularis EmuJ_000158500   expressed conserved protein
Homo sapiens ENSG00000138669   protein kinase, cGMP-dependent, type II
Homo sapiens ENSG00000185532   protein kinase, cGMP-dependent, type I
Loa Loa (eye worm) LOAG_02880   AGC/PKG protein kinase
Mus musculus ENSMUSG00000029334   protein kinase, cGMP-dependent, type II
Mus musculus 19091   protein kinase, cGMP-dependent, type I
Mycobacterium ulcerans MUL_2202   hypothetical protein
Neospora caninum NCLIV_055260   AGC kinase TgPKG1, putative
Oryza sativa 4329023   Os02g0281000
Plasmodium berghei PBANKA_1008200   cGMP-dependent protein kinase
Plasmodium falciparum PF3D7_1436600   cGMP-dependent protein kinase
Plasmodium knowlesi PKNH_0423200   cGMP-dependent protein kinase, putative
Plasmodium yoelii PY02304   cGMP-dependent protein kinase-related
Schistosoma japonicum Sjp_0046550   expressed protein
Schistosoma japonicum Sjp_0098010   cGMP-dependent protein kinase 2, putative
Schistosoma japonicum Sjp_0111040   cGMP-dependent protein kinase, isozyme 1, putative
Schistosoma japonicum Sjp_0046560   ko:K07376 protein kinase, cGMP-dependent, putative
Schistosoma japonicum Sjp_0122710   cGMP-dependent protein kinase, isozyme 1, putative
Schistosoma japonicum Sjp_0104990   cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B, putative
Schistosoma japonicum Sjp_0022170   ko:K07376 protein kinase, cGMP-dependent, putative
Schistosoma japonicum Sjp_0130620   cGMP-dependent protein kinase, isozyme 1, putative
Schistosoma mansoni Smp_123290   serine/threonine protein kinase
Schistosoma mansoni Smp_168670   serine/threonine protein kinase
Schmidtea mediterranea mk4.032121.00  
Schmidtea mediterranea mk4.021434.00  
Schmidtea mediterranea mk4.022194.00  
Schmidtea mediterranea mk4.000154.02   cGMP-dependent protein kinase 2
Schmidtea mediterranea mk4.027115.00  
Schmidtea mediterranea mk4.009551.02  
Schmidtea mediterranea mk4.000154.06  
Schmidtea mediterranea mk4.008740.03  
Schmidtea mediterranea mk4.018099.00   Serine/threonine kinase
Schmidtea mediterranea mk4.020413.00  
Schmidtea mediterranea mk4.011551.00  
Schmidtea mediterranea mk4.020413.01  
Schmidtea mediterranea mk4.009286.01  
Schmidtea mediterranea mk4.027131.00   cGMP-dependent protein kinase 1
Schmidtea mediterranea mk4.014709.00   cGMP-dependent protein kinase, isozyme 1
Schmidtea mediterranea mk4.002475.04   cGMP-dependent protein kinase egl-4
Schmidtea mediterranea mk4.035287.00   cGMP-dependent protein kinase,putative
Schmidtea mediterranea mk4.011327.00  
Toxoplasma gondii TGME49_311360   protein kinase G AGC kinase family member PKG
Theileria parva TP03_0511   cGMP-dependent protein kinase, putative

Essentiality

PY02304 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_C09G4.2 Caenorhabditis elegans sterile wormbase
TGME49_311360 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens protein kinase, cGMP-dependent, type I Compounds References
Bos taurus cGMP-dependent protein kinase 1 alpha Compounds References
Eimeria tenella cGMP-dependent protein kinase Compounds References
Plasmodium falciparum cGMP-dependent protein kinase Compounds References
Homo sapiens protein kinase, cGMP-dependent, type II Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Patiria pectinifera Cdc2 300 aa 29.8% 299 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 39.5% 291 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 38.8% 330 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 28.9% 287 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 30.6% 288 aa Compounds References
Oryctolagus cuniculus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 39.3% 295 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PY02304 (Plasmodium yoelii), cGMP-dependent protein kinase-related
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