Detailed view for Tbg972.3.3430

Basic information

TDR Targets ID: 540369
Trypanosoma brucei gambiense, ATP-dependent phosphofructokinase,6-phospho-1-fructokinase
Source Database / ID: 

pI: 9.7605 | Length (AA): 487 | MW (Da): 53517 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00365   Phosphofructokinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0003872   6-phosphofructokinase activity  
GO:0006096   glycolysis  
GO:0006002   fructose 6-phosphate metabolic process  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 485 3f5m (A) 10 485 99.99 0 1 2.16501 -1.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126758)

Species Accession Gene Product
Arabidopsis thaliana AT4G29220   6-phosphofructokinase 1
Arabidopsis thaliana AT2G22480   6-phosphofructokinase 5
Arabidopsis thaliana AT4G26270   6-phosphofructokinase 3
Arabidopsis thaliana AT5G56630   6-phosphofructokinase 7
Arabidopsis thaliana AT5G61580   6-phosphofructokinase 4
Arabidopsis thaliana AT4G32840   6-phosphofructokinase 6
Brugia malayi Bm1_31220   phosphofructokinase
Brugia malayi Bm1_01930   6-phosphofructokinase
Brugia malayi Bm1_27025   6-phosphofructokinase
Candida albicans CaO19.13893   phosphofructokinase beta-subunit that can functionally substitute for S. cerevisiae PFK2 (YMR205C)
Candida albicans CaO19_6540   hypothetical protein
Candida albicans CaO19.3967   phosphofructokinase alpha subunit that can functionally substitute for S. cerevisiae PFK1 (YGR240C)
Candida albicans CaO19.11449   phosphofructokinase alpha subunit that can functionally substitute for S. cerevisiae PFK1 (YGR240C)
Candida albicans CaO19.6540   phosphofructokinase beta-subunit that can functionally substitute for S. cerevisiae PFK2 (YMR205C)
Caenorhabditis elegans CELE_Y71H10A.1   Protein PFK-1, isoform B
Caenorhabditis elegans CELE_C50F4.2   Protein PFK-2
Dictyostelium discoideum DDB_G0274111   hypothetical protein
Drosophila melanogaster Dmel_CG4001   Phosphofructokinase
Escherichia coli b3916   6-phosphofructokinase I
Echinococcus granulosus EgrG_001128600   6 phosphofructokinase
Entamoeba histolytica EHI_103590   phosphofructokinase, putative
Entamoeba histolytica EHI_187040   phosphofructokinase, putative
Entamoeba histolytica EHI_163630   phosphofructokinase, putative
Echinococcus multilocularis EmuJ_001128600   6 phosphofructokinase
Homo sapiens ENSG00000141959   phosphofructokinase, liver
Homo sapiens ENSG00000067057   phosphofructokinase, platelet
Homo sapiens ENSG00000152556   phosphofructokinase, muscle
Leishmania braziliensis LbrM.29.2480   ATP-dependent phosphofructokinase
Leishmania donovani LdBPK_292620.1   ATP-dependent 6-phosphofructokinase, glycosomal
Leishmania infantum LinJ.29.2620   ATP-dependent phosphofructokinase
Leishmania major LmjF.29.2510   ATP-dependent phosphofructokinase
Leishmania mexicana LmxM.08_29.2510   6-phospho-1-fructokinase, putative
Loa Loa (eye worm) LOAG_00014   6-phosphofructokinase
Loa Loa (eye worm) LOAG_06199   6-phosphofructokinase
Loa Loa (eye worm) LOAG_00771   phosphofructokinase
Mycobacterium leprae ML1701c   PROBABLE 6-PHOSPHOFRUCTOKINASE PFKA (PHOSPHOHEXOKINASE) (PHOSPHOFRUCTOKINASE)
Mus musculus ENSMUSG00000021196   phosphofructokinase, platelet
Mus musculus ENSMUSG00000020277   phosphofructokinase, liver, B-type
Mus musculus ENSMUSG00000033065   phosphofructokinase, muscle
Mycobacterium tuberculosis Rv3010c   Probable 6-phosphofructokinase PfkA (phosphohexokinase) (phosphofructokinase)
Mycobacterium ulcerans MUL_1940   6-phosphofructokinase
Oryza sativa 4347060   Os09g0415800
Oryza sativa 4327301   Os01g0191700
Oryza sativa 4345675   Os08g0439000
Oryza sativa 4348582   Os10g0405600
Oryza sativa 4339369   Os05g0524400
Oryza sativa 4347383   Os09g0479800
Saccharomyces cerevisiae YGR240C   6-phosphofructokinase subunit alpha
Saccharomyces cerevisiae YMR205C   6-phosphofructokinase subunit beta
Schistosoma japonicum Sjp_0211970   ko:K00850 6-phosphofructokinase [EC2.7.1.11], putative
Schistosoma mansoni Smp_043670.2   6-phosphofructokinase
Schistosoma mansoni Smp_043670.1   6-phosphofructokinase
Schmidtea mediterranea mk4.006467.02   6-phosphofructokinase
Schmidtea mediterranea mk4.003461.03  
Schmidtea mediterranea mk4.004241.00   6-phosphofructokinase
Schmidtea mediterranea mk4.008090.00  
Schmidtea mediterranea mk4.008090.01   6-phosphofructokinase
Schmidtea mediterranea mk4.058366.01   6-phosphofructokinase
Schmidtea mediterranea mk4.004166.00  
Schmidtea mediterranea mk4.000422.07   6-phosphofructokinase
Schmidtea mediterranea mk4.041382.00  
Schmidtea mediterranea mk4.003461.01   6-phosphofructokinase
Schmidtea mediterranea mk4.004166.02   6-phosphofructokinase
Trypanosoma brucei gambiense Tbg972.3.3430   ATP-dependent phosphofructokinase,6-phospho-1-fructokinase
Trypanosoma brucei Tb927.3.3270   ATP-dependent 6-phosphofructokinase, glycosomal
Trypanosoma congolense TcIL3000_0_42400   ATP-dependent 6-phosphofructokinase, glycosomal
Trypanosoma cruzi TcCLB.508153.340   ATP-dependent 6-phosphofructokinase, glycosomal
Treponema pallidum TP0108   diphosphate--fructose-6-phosphate 1-phosphotransferase
Trichomonas vaginalis TVAG_462920   phosphofructokinase, putative
Trichomonas vaginalis TVAG_293770   phosphofructokinase, putative
Trichomonas vaginalis TVAG_496160   phosphofructokinase, putative
Trichomonas vaginalis TVAG_391760   phosphofructokinase, putative

Essentiality

Tbg972.3.3430 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu3061 Mycobacterium tuberculosis non-essential nmpdr
Tb927.3.3270 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.3.3270 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.3270 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.3270 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3916 Escherichia coli non-essential goodall
CELE_Y71H10A.1 Caenorhabditis elegans embryonic lethal wormbase
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

2767 chemical compounds are associated with this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma cruzi ATP-dependent 6-phosphofructokinase, glycosomal Compounds References
Schistosoma mansoni 6-phosphofructokinase Compounds References
Trypanosoma brucei ATP-dependent 6-phosphofructokinase, glycosomal Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • ChEMBL
  • PUBCHEM_BIOASSAY: Tb PFK orthogonal confirmatory assay using ATP depletion (Kinase-Glo Plus) as an alternative measure of Tb PFK activity: Hit Validation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488768]
  • ChEMBL
  • PUBCHEM_BIOASSAY: Inhibitors of T. brucei phosphofructokinase: Hit Validation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488768]
  • ChEMBL
  • PUBCHEM_BIOASSAY: qHTS Validation Assay to Find Inhibitors of T. brucei phosphofructokinase. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488768, AID492961]
  • ChEMBL
  • PUBCHEM_BIOASSAY: qHTS Assay to Find Inhibitors of T. brucei phosphofructokinase. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488768, AID492961]
  • ChEMBL
  • PUBCHEM_BIOASSAY: qHTS Assay to Find Inhibitors of T. brucei phosphofructokinase: hit validation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID485367, AID485368, AID488768]
  • ChEMBL
  • Inhibition of Trypanosoma brucei phosphofructokinase assessed as enzyme residual activity at 5 mM
  • ChEMBL
  • Inhibition of Trypanosoma brucei phosphofructokinase
  • ChEMBL
  • Inhibition of Trypanosoma brucei phosphofructokinase assessed as enzyme residual activity at 1 mM
  • ChEMBL
  • Inhibition of Trypanosoma brucei PFK expressed in Escherichia coli
  • ChEMBL
  • Inhibition of Trypanosoma brucei PFK expressed in Escherichia coli at 690 uM
  • ChEMBL
  • Competitive reversible inhibition of Trypanosoma brucei PFK expressed in Escherichia coli using fructose-6-phosphate by spectrophotometry analysis
  • ChEMBL
  • Ratio of Ki/Km for Trypanosoma brucei PFK expressed in Escherichia coli using fructose-6-phosphate by spectrophotometry analysis
  • ChEMBL
  • Mixed type inhibition Trypanosoma brucei PFK using ATP as substrate by Line-weaver Burk plot analysis
  • ChEMBL
  • Competitive inhibition Trypanosoma brucei PFK using fructose-6-phosphate as substrate by Line-weaver Burk plot analysis
  • ChEMBL
  • Inhibition of Trypanosoma brucei PFK-mediated ADP production using ATP/fructose-6-phosphate as substrate at 57 uM by luciferase based luminescence assay
  • ChEMBL
  • Inhibition of Trypanosoma brucei PFK-mediated ADP production using ATP/fructose-6-phosphate as substrate by luciferase based luminescence assay

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Tbg972.3.3430 (Trypanosoma brucei gambiense), ATP-dependent phosphofructokinase,6-phospho-1-fructokinase
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