Detailed view for Tbg972.10.4060

Basic information

TDR Targets ID: 541100
Trypanosoma brucei gambiense, delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Source Database / ID: 

pI: 7.4108 | Length (AA): 561 | MW (Da): 62009 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00171   Aldehyde dehydrogenase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016620   oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor  
GO:0010133   proline catabolic process to glutamate  
GO:0016491   oxidoreductase activity  
GO:0003842   1-pyrroline-5-carboxylate dehydrogenase activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 554 4e3x (A) 21 562 44.00 0 1 1.48822 -0.76
11 557 4jdc (A) 3 543 44.00 0 1 1.54784 -0.72
42 543 4oe6 (A) 53 562 52.00 0 1 1.48063 -0.55
52 542 1ag8 (A) 22 494 23.00 0.00012 1 1.08592 -0.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128522)

Species Accession Gene Product
Brugia malayi Bm1_04205   Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial precursor
Candida albicans CaO19.3974   delta-1-pyrroline-5-carboxylate dehydrogenase
Candida albicans CaO19.11457   delta-1-pyrroline-5-carboxylate dehydrogenase
Caenorhabditis elegans CELE_F56D12.1   Protein ALH-6, isoform D
Dictyostelium discoideum DDB_G0283293   hypothetical protein
Drosophila melanogaster Dmel_CG7145   delta-1-Pyrroline-5-carboxylate dehydrogenase 1
Escherichia coli b1014   fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase
Homo sapiens ENSG00000159423   aldehyde dehydrogenase 4 family, member A1
Leishmania braziliensis LbrM.03.0210   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Leishmania donovani LdBPK_030190.1   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Leishmania infantum LinJ.03.0190   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Leishmania major LmjF.03.0200   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Leishmania mexicana LmxM.03.0200   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Loa Loa (eye worm) LOAG_07106   delta-1-pyrroline-5-carboxylate dehydrogenase
Mus musculus ENSMUSG00000028737   aldehyde dehydrogenase 4 family, member A1
Mycobacterium tuberculosis Rv1187   Probable pyrroline-5-carboxylate dehydrogenase RocA
Mycobacterium ulcerans MUL_0932   pyrroline-5-carboxylate dehydrogenase, RocA
Saccharomyces cerevisiae YHR037W   1-pyrroline-5-carboxylate dehydrogenase
Schistosoma japonicum Sjp_0107580   expressed protein
Schistosoma japonicum Sjp_0203100   ko:K00294 1-pyrroline-5-carboxylate dehydrogenase [EC1.5.1.12], putative
Schistosoma mansoni Smp_065060   aldehyde dehydrogenase
Schmidtea mediterranea mk4.000753.03   Putative aldehyde dehydrogenase
Trypanosoma brucei gambiense Tbg972.10.4060   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Trypanosoma brucei Tb927.10.3210   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Trypanosoma congolense TcIL3000_10_2660   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Trypanosoma cruzi TcCLB.510943.50   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Trypanosoma cruzi TcCLB.509351.10   delta-1-pyrroline-5-carboxylate dehydrogenase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0539   bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase

Essentiality

Tbg972.10.4060 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1208 Mycobacterium tuberculosis essential nmpdr
Tb927.10.3210 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.3210 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.3210 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.3210 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1014 Escherichia coli non-essential goodall
CELE_F56D12.1 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Aldehyde dehydrogenase 519 aa 28.4% 454 aa Compounds References
Rattus norvegicus Aldehyde dehydrogenase 1A1 501 aa 26.9% 453 aa Compounds References
Rattus norvegicus Aldehyde dehydrogenase, cytosolic 1 501 aa 26.7% 454 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Tbg972.10.4060 (Trypanosoma brucei gambiense), delta-1-pyrroline-5-carboxylate dehydrogenase, putative
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