Detailed view for Tbg972.3.300

Basic information

TDR Targets ID: 541406
Trypanosoma brucei gambiense, protein kinase, putative
Source Database / ID: 

pI: 9.818 | Length (AA): 583 | MW (Da): 63808 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 444 3pvu (A) 188 604 18.00 0 1 0.706078 0.49
1 304 5d1j (A) 1 287 45.00 0 1 0.856541 -0.08
268 304 5my8 (A) 618 654 32.00 0 0.24 0.404965 -0.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127559)

Species Accession Gene Product
Arabidopsis thaliana AT5G45430   protein kinase superfamily protein
Arabidopsis thaliana AT4G19110   conserved peptide upstream open reading frame 25
Candida albicans CaO19.9931   serine/threonine kinase, positive regulator of meiosis
Candida albicans CaO19.2395   serine/threonine kinase, positive regulator of meiosis
Caenorhabditis elegans CELE_M04C9.5   Protein DYF-5, isoform B
Dictyostelium discoideum DDB_G0268078   RCK family protein kinase
Drosophila melanogaster Dmel_CG42366   CG42366 gene product from transcript CG42366-RA
Echinococcus granulosus EgrG_000591900   serine:threonine protein kinase MAK
Echinococcus multilocularis EmuJ_000591900   serine:threonine protein kinase MAK
Giardia lamblia GL50803_6700   Kinase, CMGC RCK
Homo sapiens ENSG00000111837   male germ cell-associated kinase
Homo sapiens ENSG00000112144   intestinal cell (MAK-like) kinase
Leishmania braziliensis LbrM.27.0110   protein kinase, putative
Leishmania braziliensis LbrM.19.0490   mitogen activated protein kinase, putative,map kinase, putative
Leishmania donovani LdBPK_270100.1   protein kinase, putative
Leishmania donovani LdBPK_190170.1   mitogen-activated protein kinase 9, putative
Leishmania infantum LinJ.19.0170   mitogen activated protein kinase, putative,map kinase, putative
Leishmania infantum LinJ.27.0100   protein kinase, putative
Leishmania major LmjF.27.0100   protein kinase, putative
Leishmania major LmjF.19.0180   mitogen activated protein kinase, putative,map kinase, putative
Leishmania mexicana LmxM.19.0180   mitogen activated protein kinase, putative,map kinase, putative
Leishmania mexicana LmxM.27.0100   protein kinase, putative
Loa Loa (eye worm) LOAG_06734   hypothetical protein
Loa Loa (eye worm) LOAG_12455   CMGC/RCK/MAK protein kinase
Mus musculus ENSMUSG00000009828   intestinal cell kinase
Mus musculus ENSMUSG00000021363   male germ cell-associated kinase
Oryza sativa 4330434   Os02g0700600
Oryza sativa 4339927   Os06g0116100
Saccharomyces cerevisiae YJL106W   Ime2p
Schistosoma japonicum Sjp_0307390   ko:K03127 transcription initiation factor TFIID subunit D11, putative
Schistosoma japonicum Sjp_0065020   Oxysterol-binding protein-related protein 9, putative
Schistosoma japonicum Sjp_0098600   IPR000719,Protein kinase;IPR006163,Phosphopantetheine-binding;IPR011009,Protein kinase-like,domain-containing
Schistosoma mansoni Smp_132890   serine/threonine protein kinase
Schmidtea mediterranea mk4.002465.00  
Schmidtea mediterranea mk4.001636.02  
Trypanosoma brucei gambiense Tbg972.10.17970   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.3.300   protein kinase, putative
Trypanosoma brucei Tb927.3.690   CMGC/RCK protein kinase, putative
Trypanosoma brucei Tb927.10.14800   mitogen-activated protein kinase 9, putative
Trypanosoma congolense TcIL3000_10_12670   mitogen-activated protein kinase 9, putative
Trypanosoma cruzi TcCLB.509719.50   CMGC/RCK protein kinase, putative
Trypanosoma cruzi TcCLB.506211.180   mitogen-activated protein kinase 9, putative
Trypanosoma cruzi TcCLB.509231.20   CMGC/RCK protein kinase, putative
Trypanosoma cruzi TcCLB.511289.50   mitogen-activated protein kinase 9, putative
Trichomonas vaginalis TVAG_129430   CMGC family protein kinase
Trichomonas vaginalis TVAG_529130   CMGC family protein kinase
Trichomonas vaginalis TVAG_399660   CMGC family protein kinase
Trichomonas vaginalis TVAG_583940   CMGC family protein kinase

Essentiality

Tbg972.3.300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.690 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.3.690 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.690 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.690 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.14800 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.14800 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.14800 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.14800 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens intestinal cell (MAK-like) kinase Compounds References
Homo sapiens male germ cell-associated kinase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Mitogen-activated protein kinase 8 411 aa 30.7% 349 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 22.3% 309 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 25.2% 294 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 22.3% 309 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 26.8% 314 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 33.9% 313 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 33.1% 338 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 25.9% 294 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 32.5% 317 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 39.4% 180 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 37.7% 310 aa Compounds References
Patiria pectinifera Cdc2 300 aa 37.0% 311 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 37.9% 311 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 25.5% 188 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tbg972.3.300 (Trypanosoma brucei gambiense), protein kinase, putative
Title for this comment
Comment