Detailed view for TcCLB.509669.70

Basic information

TDR Targets ID: 54317
Trypanosoma cruzi, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.8314 | Length (AA): 647 | MW (Da): 73101 | Paralog Number: 3

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 10

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02990   Endomembrane protein 70

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
261 357 2wyo (A) 333 435 33.00 0.78 0.11 0.160023 1.73
346 553 4u4v (A) 255 453 15.00 0 0.02 0.448684 -0.39
346 619 2cfq (A) 73 323 18.00 0 0.85 0.351693 2.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_127047)

Species Accession Gene Product
Arabidopsis thaliana AT5G25100   endomembrane family protein 70
Arabidopsis thaliana AT3G13772   protein transmembrane nine 7
Arabidopsis thaliana AT5G10840   endomembrane family protein 70
Arabidopsis thaliana AT1G55130   transmembrane nine protein 6
Arabidopsis thaliana AT2G24170   Endomembrane protein 70 family protein
Brugia malayi Bm1_34290   Transmembrane 9 superfamily protein member 2 precursor, putative
Brugia malayi Bm1_22910   Transmembrane 9 superfamily protein member 4
Candida albicans CaO19.10260   Endosomal membrane protein, possible ion transporter
Candida albicans CaO19.2746   Endosomal membrane protein, possible ion transporter
Caenorhabditis elegans CELE_F08F1.7   Protein TAG-123
Caenorhabditis elegans CELE_ZK858.6   Protein ZK858.6, isoform A
Dictyostelium discoideum DDB_G0267444   TM9 protein A
Drosophila melanogaster Dmel_CG9318   CG9318 gene product from transcript CG9318-RB
Drosophila melanogaster Dmel_CG7364   CG7364 gene product from transcript CG7364-RB
Echinococcus granulosus EgrG_000488300   Nonaspanin TM9SF
Echinococcus granulosus EgrG_000530300   Transmembrane 9 superfamily protein
Echinococcus multilocularis EmuJ_000488300   Nonaspanin (TM9SF)
Echinococcus multilocularis EmuJ_000530300   Transmembrane 9 superfamily protein
Homo sapiens ENSG00000101337   transmembrane 9 superfamily protein member 4
Homo sapiens ENSG00000125304   transmembrane 9 superfamily member 2
Leishmania braziliensis LbrM.29.1710   hypothetical protein, conserved
Leishmania braziliensis LbrM.30.0400   endosomal integral membrane protein, putative
Leishmania donovani LdBPK_291730.1   endomembrane protein 70, putative
Leishmania donovani LdBPK_300360.1   endosomal integral membrane protein, putative
Leishmania infantum LinJ.30.0360   endosomal integral membrane protein, putative
Leishmania infantum LinJ.29.1730   hypothetical protein, conserved
Leishmania major LmjF.29.1600   hypothetical protein, conserved
Leishmania major LmjF.30.0360   endosomal integral membrane protein, putative
Leishmania mexicana LmxM.29.0360   endosomal integral membrane protein, putative
Leishmania mexicana LmxM.08_29.1600   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_03420   transmembrane 9 superfamily protein member 4
Loa Loa (eye worm) LOAG_00899   TAG-123 protein
Mus musculus ENSMUSG00000068040   transmembrane 9 superfamily protein member 4
Mus musculus ENSMUSG00000079584   predicted gene 364
Mus musculus ENSMUSG00000025544   transmembrane 9 superfamily member 2
Neospora caninum NCLIV_042810   hypothetical protein
Oryza sativa 4347829   Os09g0557800
Oryza sativa 9266768   Os08g0276000
Oryza sativa 9267678   Os08g0555001
Oryza sativa 4346279   Os08g0555200
Oryza sativa 9271128   Os08g0555300
Oryza sativa 4346277   Os08g0554900
Oryza sativa 4341338   Os06g0568000
Oryza sativa 4341685   Os06g0650600
Onchocerca volvulus OVOC1277   Transmembrane 9 superfamily member 4 homolog
Plasmodium berghei PBANKA_0505600   endomembrane protein 70, putative
Plasmodium falciparum PF3D7_1021400   endomembrane protein 70, putative
Plasmodium knowlesi PKNH_0605700   endomembrane protein 70, putative
Plasmodium vivax PVX_001935   endomembrane protein 70, putative
Plasmodium yoelii PY02254   multispanning membrane protein, putative-related
Saccharomyces cerevisiae YLR083C   Emp70p
Saccharomyces cerevisiae YDR107C   Tmn2p
Schistosoma japonicum Sjp_0205100   Transmembrane 9 superfamily member 2 precursor, putative
Schistosoma japonicum Sjp_0028860   Transmembrane 9 superfamily member 2 precursor, putative
Schistosoma japonicum Sjp_0112090   hypothetical protein
Schistosoma japonicum Sjp_0300860   Transmembrane 9 superfamily member 4 precursor, putative
Schistosoma mansoni Smp_163130   transmembrane 9 superfamily protein member
Schistosoma mansoni Smp_162040   transmembrane 9 superfamily member
Schmidtea mediterranea mk4.006637.01   Transmembrane 9 superfamily member 2
Schmidtea mediterranea mk4.006637.00   Transmembrane 9 superfamily member 2
Schmidtea mediterranea mk4.000768.03  
Schmidtea mediterranea mk4.026229.00   Transmembrane 9 superfamily member 2
Schmidtea mediterranea mk4.000649.00   Putative transmembrane 9 superfamily protein member
Trypanosoma brucei gambiense Tbg972.3.4610   endosomal integral membrane protein, putative
Trypanosoma brucei gambiense Tbg972.8.1580   endosomal integral membrane protein, putative
Trypanosoma brucei Tb927.3.4190   endosomal integral membrane protein, putative
Trypanosoma brucei Tb927.8.1940   endosomal integral membrane protein, putative
Trypanosoma congolense TcIL3000_8_1890   endosomal integral membrane protein, putative
Trypanosoma congolense TcIL3000_0_02910   endosomal integral membrane protein, putative
Trypanosoma cruzi TcCLB.511491.70   endosomal integral membrane protein, putative
Trypanosoma cruzi TcCLB.509669.70   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511391.90   endosomal integral membrane protein, putative
Trypanosoma cruzi TcCLB.506175.20   hypothetical protein, conserved
Trichomonas vaginalis TVAG_157240   endomembrane protein emp70, putative
Trichomonas vaginalis TVAG_066430   endomembrane protein emp70, putative
Trichomonas vaginalis TVAG_192830   endosomal P24a protein, putative

Essentiality

TcCLB.509669.70 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.4190 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.4190 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.4190 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.3.4190 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.1940 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.1940 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.1940 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.1940 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.509669.70 (Trypanosoma cruzi), hypothetical protein, conserved
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