Detailed view for TcCLB.504105.140

Basic information

TDR Targets ID: 54598
Trypanosoma cruzi, enolase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.1875 | Length (AA): 429 | MW (Da): 46444 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00113   Enolase, C-terminal TIM barrel domain
PF03952   Enolase, N-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0000015   phosphopyruvate hydratase complex  
GO:0046872   metal ion binding  
GO:0004634   phosphopyruvate hydratase activity  
GO:0000287   magnesium ion binding  
GO:0006096   glycolysis  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 429 4g7f (A) 1 429 99.99 0 1 2.2175 -1.45

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 4G7F:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126698)

Species Accession Gene Product
Arabidopsis thaliana AT2G36530   bifunctional enolase 2/transcriptional activator
Arabidopsis thaliana AT1G74030   enolase 1
Arabidopsis thaliana AT2G29560   putative phosphoenolpyruvate enolase
Babesia bovis BBOV_III007950   enolase (2-phosphoglycerate dehydratase)
Brugia malayi Bm1_24115   enolase
Candida albicans CaO19.8025   enolase similar to S. cerevisiae ENO1 (YGR254W), gluconeogenesis
Candida albicans CaO19.395   enolase similar to S. cerevisiae ENO1 (YGR254W), gluconeogenesis
Caenorhabditis elegans CELE_T21B10.2   Protein ENOL-1, isoform A
Cryptosporidium hominis Chro.50184   enolase
Cryptosporidium parvum cgd5_1960   enolase (2-phosphoglycerate dehydratase)
Chlamydia trachomatis CT_587   enolase
Dictyostelium discoideum DDB_G0268214   2-phospho-D-glycerate hydrolase
Dictyostelium discoideum DDB_G0283137   2-phospho-D-glycerate hydrolase
Drosophila melanogaster Dmel_CG17654   Enolase
Escherichia coli b2779   enolase
Echinococcus granulosus EgrG_000514200   enolase
Echinococcus granulosus EgrG_000179700   enolase
Entamoeba histolytica EHI_130700   enolase, putative
Echinococcus multilocularis EmuJ_000514200   enolase
Echinococcus multilocularis EmuJ_000533500   enolase
Echinococcus multilocularis EmuJ_000179700   enolase
Giardia lamblia GL50803_11118   Enolase
Homo sapiens ENSG00000111674   enolase 2 (gamma, neuronal)
Homo sapiens ENSG00000074800   enolase 1, (alpha)
Homo sapiens ENSG00000108515   enolase 3 (beta, muscle)
Leishmania braziliensis LbrM.14.1330   enolase
Leishmania braziliensis LbrM.14.1320   enolase
Leishmania donovani LdBPK_141240.1   enolase
Leishmania infantum LinJ.14.1240   enolase
Leishmania major LmjF.14.1160   enolase
Leishmania mexicana LmxM.14.1160   enolase
Loa Loa (eye worm) LOAG_14155   enolase
Loa Loa (eye worm) LOAG_15787   enolase
Loa Loa (eye worm) LOAG_13911   enolase
Loa Loa (eye worm) LOAG_00912   enolase
Mycobacterium leprae ML0255   Probable enolase Eno (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate hydro-lyase)
Mus musculus ENSMUSG00000060600   enolase 3, beta muscle
Mus musculus ENSMUSG00000059040   enolase 1B, retrotransposed
Mus musculus ENSMUSG00000004267   enolase 2, gamma neuronal
Mus musculus ENSMUSG00000063524   enolase 1, alpha non-neuron
Mus musculus 102642340   alpha-enolase-like
Mycobacterium tuberculosis Rv1023   Probable enolase Eno
Mycobacterium ulcerans MUL_4631   phosphopyruvate hydratase
Neospora caninum NCLIV_037490   Enolase (EC 4.2.1.11), related
Neospora caninum NCLIV_037500   Enolase (EC 4.2.1.11), related
Oryza sativa 4346906   Os09g0375000
Oryza sativa 4332253   Os03g0248600
Oryza sativa 4340042   Os06g0136600
Oryza sativa 4348176   Os10g0167300
Onchocerca volvulus OVOC10026   Enolase homolog
Onchocerca volvulus OVOC9778   Enolase homolog
Plasmodium berghei PBANKA_1214300   enolase, putative
Plasmodium falciparum PF3D7_1015900   enolase
Plasmodium knowlesi PKNH_0816200   enolase, putative
Plasmodium vivax PVX_095015   enolase, putative
Plasmodium yoelii PY06644   enolase
Saccharomyces cerevisiae YHR174W   phosphopyruvate hydratase ENO2
Saccharomyces cerevisiae YOR393W   phosphopyruvate hydratase ERR1
Saccharomyces cerevisiae YGR254W   phosphopyruvate hydratase ENO1
Saccharomyces cerevisiae YMR323W   phosphopyruvate hydratase ERR3
Saccharomyces cerevisiae YPL281C   phosphopyruvate hydratase ERR2
Schistosoma japonicum Sjp_0209670   ko:K01689 enolase [EC4.2.1.11], putative
Schistosoma mansoni Smp_024110   phosphopyruvate hydratase
Schmidtea mediterranea mk4.001125.11  
Schmidtea mediterranea mk4.001125.12   Enolase
Schmidtea mediterranea mk4.034045.01   Enolase
Schmidtea mediterranea mk4.005541.03   Enolase
Schmidtea mediterranea mk4.002232.01   Enolase
Schmidtea mediterranea mk4.001125.14   Enolase
Schmidtea mediterranea mk4.054382.00   Enolase
Trypanosoma brucei gambiense Tbg972.10.3600   enolase, putative
Trypanosoma brucei gambiense Tbg972.10.3610   enolase
Trypanosoma brucei Tb927.10.2890   enolase
Trypanosoma congolense TcIL3000_10_2500   enolase, putative
Trypanosoma cruzi TcCLB.504105.140   enolase
Toxoplasma gondii TGME49_268860   enolase 1
Toxoplasma gondii TGME49_268850   enolase 2
Treponema pallidum TP0817   phosphopyruvate hydratase
Theileria parva TP04_0700   enolase, putative
Trichomonas vaginalis TVAG_263740   enolase, putative
Trichomonas vaginalis TVAG_043500   enolase, putative
Trichomonas vaginalis TVAG_358110   enolase, putative
Trichomonas vaginalis TVAG_464170   enolase, putative
Trichomonas vaginalis TVAG_487600   enolase, putative
Trichomonas vaginalis TVAG_329460   enolase, putative
Trichomonas vaginalis TVAG_282090   enolase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0119   phosphopyruvate hydratase

Essentiality

TcCLB.504105.140 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.2890 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.2890 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.2890 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.2890 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b2779 Escherichia coli essential goodall
CELE_T21B10.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_T21B10.2 Caenorhabditis elegans larval arrest wormbase
CELE_T21B10.2 Caenorhabditis elegans larval lethal wormbase
CELE_T21B10.2 Caenorhabditis elegans slow growth wormbase
CELE_T21B10.2 Caenorhabditis elegans sterile wormbase
PBANKA_1214300 Plasmodium berghei Essential plasmo
TGME49_268860 Toxoplasma gondii Probably essential sidik
TGME49_268850 Toxoplasma gondii Probably essential sidik
TGME49_268860 Toxoplasma gondii Probably non-essential sidik
TGME49_268850 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens enolase 2 (gamma, neuronal) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Enolase (4.2.1.11 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

5 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TcCLB.504105.140 (Trypanosoma cruzi), enolase
Title for this comment
Comment