Detailed view for PF3D7_1243900

Basic information

TDR Targets ID: 5476
Plasmodium falciparum, double C2-like domain-containing protein
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.5971 | Length (AA): 1846 | MW (Da): 221637 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00168   C2 domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 12 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
498 627 1rh8 (A) 4635 4775 22.00 0 0.99 0.16 -0.18
503 608 1w15 (A) 288 394 16.00 0.0000003 0.45 0.08 -0.71
1223 1676 1f5n (A) 132 582 16.00 0 0.81 -0.11 1.77
324 458 2enp (A) 1 136 7.00 0.51 0.01 0.0512311 0.32
334 472 3n5a (A) 266 400 6.00 0.023 0 0.0923979 -0.08
516 607 2cm5 (A) 557 646 30.00 0.37 0.76 0.187837 0.5
1339 1514 4uos (A) 3 183 33.00 0.0023 0.21 0.451941 -1.4
1445 1667 4tql (A) 9 238 27.00 0.0000001 0.2 0.521402 -1.85
1531 1612 4o9b (A) 253 334 29.00 0.57 0 0.58402 -3.14
1371 1833 4h5y (A) 62 521 27.00 0.00045 0.86 0.29033 0.93
1449 1606 4wik (A) 447 595 24.00 0.00089 0.15 0.39857 -1.32
1549 1626 4o9b (A) 253 334 29.00 0.56 0.06 0.584091 -3.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Female Gametocyte, Male Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile gametocyte. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Oocyst, Ring, Sporozoite. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs. Otto TD
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_141554)

Species Accession Gene Product
Babesia bovis BBOV_III003050   conserved hypothetical protein
Cryptosporidium hominis Chro.70342   hypothetical protein
Cryptosporidium parvum cgd7_3040   membrane protein conserved in eukaryotes
Neospora caninum NCLIV_017060   C2 domain-containing protein, putative
Plasmodium berghei PBANKA_1457200   double C2-like domain-containing protein, putative
Plasmodium falciparum PF3D7_1243900   double C2-like domain-containing protein
Plasmodium knowlesi PKNH_1463200   double C2-like domain-containing protein, putative
Plasmodium vivax PVX_101095   double C2-like domain-containing protein, putative
Plasmodium yoelii PY01213   C2 domain, putative
Toxoplasma gondii TGME49_240910   hypothetical protein
Theileria parva TP04_0140   hypothetical protein

Essentiality

PF3D7_1243900 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_240910 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.005 0.2612 0
0.0072 0.2639 0
0.0109 0.4866 0.5
0.0058 0.5 0.5
0.0057 0.3491 0.5
0.0107 0.5 0.5
0.0071 0.2549 0
0.0121 1 1
0.0125 0.3893 1
0.0018 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_1243900 (Plasmodium falciparum), double C2-like domain-containing protein
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