Detailed view for TcCLB.460127.20

Basic information

TDR Targets ID: 54800
Trypanosoma cruzi, metallo- peptidase, Clan M- Family M48
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.7336 | Length (AA): 428 | MW (Da): 48436 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 5

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01435   Peptidase family M48
PF16491   CAAX prenyl protease N-terminal, five membrane helices

Gene Ontology

Mouse over links to read term descriptions.
GO:0071586   GO:CAAX-box protein processing  

GO:0016020   membrane  
GO:0008233   peptidase activity  
GO:0004222   metalloendopeptidase activity  
GO:0006508   proteolysis  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 421 4il3 (A) 9 445 32.00 0 1 1.2903 0.64
10 420 2ypt (B) 20 469 36.00 0 1 1.30948 0.45
216 301 3cqb (A) 20 100 18.00 0 0.86 0.425035 -0.73
329 426 3c37 (A) 116 224 39.00 0.0018 0.27 0.181172 1.59

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127953)

Species Accession Gene Product
Arabidopsis thaliana AT4G01320   CAAX prenyl protease 1 homolog
Babesia bovis BBOV_IV000310   CAAX metallo endopeptidase, putative
Brugia malayi Bm1_22180   Peptidase family M48 containing protein
Candida albicans CaO19.13099   likely metallopeptidase similar to S. cerevisiae STE24 (YJR117W) involved in the proteolytic maturation of a-factor
Candida albicans CaO19.5654   likely metallopeptidase similar to S. cerevisiae STE24 (YJR117W) involved in the proteolytic maturation of a-factor
Caenorhabditis elegans CELE_C04F12.10   Protein FCE-1
Cryptosporidium hominis Chro.60017   CAAX prenyl protease
Cryptosporidium parvum cgd6_70   conserved hypothetical protein
Dictyostelium discoideum DDB_G0290849   CAAX prenyl protease
Drosophila melanogaster Dmel_CG9000   prenyl protease type I
Echinococcus granulosus EgrG_001023600   caax prenyl protease 1
Entamoeba histolytica EHI_075660   CAAX prenyl protease, putative
Echinococcus multilocularis EmuJ_001023600   caax prenyl protease 1
Homo sapiens ENSG00000084073   zinc metallopeptidase STE24
Leishmania braziliensis LbrM.27.0050   CAAX prenyl protease 1, putative,metallo-peptidase, Clan M-, Family M48
Leishmania donovani LdBPK_270040.1   metallo- peptidase, Clan M- Family M48
Leishmania infantum LinJ.27.0040   CAAX prenyl protease 1, putative,metallo-peptidase, Clan M-, Family M48
Leishmania major LmjF.27.0040   CAAX prenyl protease 1, putative,metallo-peptidase, Clan M-, Family M48
Leishmania mexicana LmxM.27.0040   CAAX prenyl protease 1, putative,metallo-peptidase, Clan M-, Family M48
Loa Loa (eye worm) LOAG_09493   peptidase family M48 containing protein
Mus musculus ENSMUSG00000043207   zinc metallopeptidase, STE24
Neospora caninum NCLIV_004750   peptidase family M48 domain-containing protein, putative
Oryza sativa 4330318   Os02g0680400
Saccharomyces cerevisiae YJR117W   Ste24p
Schistosoma japonicum Sjp_0042800   ko:K06013 STE24 endopeptidase [EC3.4.24.84], putative
Schistosoma mansoni Smp_082620   farnesylated-protein converting enzyme 1 (M48 family)
Schmidtea mediterranea mk4.002937.00   CAAX prenyl protease 1 homolog
Schmidtea mediterranea mk4.002529.03   CAAX prenyl protease 1 homolog
Schmidtea mediterranea mk4.038149.04   CAAX prenyl protease 1 homolog
Schmidtea mediterranea mk4.003114.06   CAAX prenyl protease 1 homolog
Schmidtea mediterranea mk4.003114.05  
Trypanosoma brucei gambiense Tbg972.9.4950   CAAX prenyl protease 1, putative,metallo- peptidase, Clan M- Family M48
Trypanosoma brucei Tb927.9.8950   metallo- peptidase, Clan M- Family M48
Trypanosoma congolense TcIL3000_9_3130   metallo- peptidase, Clan M- Family M48
Trypanosoma cruzi TcCLB.460127.20   metallo- peptidase, Clan M- Family M48
Trypanosoma cruzi TcCLB.510431.40   CAAX prenyl protease 1, putative
Toxoplasma gondii TGME49_221170   CAAX metallo endopeptidase
Theileria parva TP02_0928   CAAX prenyl protease 1, putative
Trichomonas vaginalis TVAG_077490   Clan MA, family M48, Ste24 endopeptidase-like metallopeptidase
Trichomonas vaginalis TVAG_381360   Clan MA, family M48, Ste24 endopeptidase-like metallopeptidase

Essentiality

TcCLB.460127.20 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.0680 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.0680 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.0680 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.0680 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_221170 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus zinc metallopeptidase, STE24 Compounds References
Saccharomyces cerevisiae Ste24p Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.008 0.3091 0.4439
0.0082 0.2504 1
0.0074 0.2695 0.6869
0.0079 0.7984 0.5
0.0079 0.3091 0
0.0066 0.354 1
0.0067 0.3711 0
0.0082 0.354 1
0.0079 0.7984 0.5
0.0076 0.384 1
0.0074 0.3362 1
0.0082 0.3815 0.7146
0.0082 0.3657 0.5
0.007 1 1
0.0071 0.2889 0.5231
0.0082 0.3436 0.5
0.0079 0.7984 0.5
0.0068 0.3148 1
0.0082 0.3513 1
0.0079 0.7984 0.5
0.0082 0.4786 0
0.0082 0.3091 0
0.0076 0.2761 1
0.007 0.3461 1
0.0073 0.3933 0.5
0.0072 1 1
0.0082 0.3899 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.460127.20 (Trypanosoma cruzi), metallo- peptidase, Clan M- Family M48
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