Detailed view for TcIL3000_10_4410

Basic information

TDR Targets ID: 549959
Trypanosoma congolense, mitogen-activated protein kinase kinase 5
Source Database / ID: 

pI: 5.0729 | Length (AA): 377 | MW (Da): 41663 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
21 321 3sls (B) 43 329 40.00 0 1 1.20491 -0.46
44 377 3q5i (A) 36 385 23.00 0 1 0.991742 -0.05
44 345 2a2a (A) 2 304 25.00 0 1 0.972861 -0.41
227 263 2zko (A) 8 46 46.00 0.57 0.26 0.579643 -0.65

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127304)

Species Accession Gene Product
Arabidopsis thaliana AT5G56580   mitogen-activated protein kinase kinase 6
Arabidopsis thaliana AT4G26070   mitogen-activated protein kinase kinase 1
Arabidopsis thaliana AT4G29810   mitogen-activated protein kinase kinase 2
Brugia malayi Bm1_48560   Dual specificity mitogen-activated protein kinase kinase mek-2
Candida albicans CaO19.8100   likely MAP kinase kinase that can functionally substitute for S. cerevisiae STE7 (YDL159W)
Candida albicans CaO19.7388   member of the HOG1 MAPK cascade
Candida albicans CaO19.469   likely MAP kinase kinase that can functionally substitute for S. cerevisiae STE7 (YDL159W)
Caenorhabditis elegans CELE_Y54E10BL.6   Protein MEK-2
Cryptosporidium hominis Chro.80584   protein kinase NPK2 (EC 2.7.1.-)
Cryptosporidium parvum cgd8_5120   protein kinase NPK2
Dictyostelium discoideum DDB_G0269152   MAP kinase kinase
Drosophila melanogaster Dmel_CG15793   Downstream of raf1
Echinococcus granulosus EgrG_000514400   dual specificity mitogen activated protein
Echinococcus multilocularis EmuJ_000514400   dual specificity mitogen activated protein
Giardia lamblia GL50803_22165   Kinase, STE STE20
Homo sapiens 5604   mitogen-activated protein kinase kinase 1
Homo sapiens ENSG00000126934   mitogen-activated protein kinase kinase 2
Leishmania braziliensis LbrM.35.0980   mitogen-activated protein kinase kinase 5, putative;with=GeneDB:LmxM36.0860
Leishmania donovani LdBPK_360920.1   mitogen-activated protein kinase kinase 5
Leishmania infantum LinJ.36.0920   mitogen-activated protein kinase kinase 5, putative;with=GeneDB:LmxM36.0860
Leishmania major LmjF.36.0860   mitogen-activated protein kinase kinase 5, putative;with=GeneDB:LmxM36.0860
Leishmania mexicana LmxM.36.0860   protein kinase, putative,mitogen activated protein kinase, putative
Loa Loa (eye worm) LOAG_12861   STE/STE7/MEK1 protein kinase
Mus musculus ENSMUSG00000035027   mitogen-activated protein kinase kinase 2
Mus musculus ENSMUSG00000004936   mitogen-activated protein kinase kinase 1
Oryza sativa 4340126   Os06g0147800
Oryza sativa 4324023   Os01g0510100
Saccharomyces cerevisiae YJL128C   Pbs2p
Saccharomyces cerevisiae YDL159W   Ste7p
Schistosoma japonicum Sjp_0095630   Dual specificity mitogen-activated protein kinase kinase 2, putative
Schistosoma japonicum Sjp_0027360   Dual specificity mitogen-activated protein kinase kinase 1, putative
Schistosoma mansoni Smp_026510   protein kinase
Schmidtea mediterranea mk4.014624.02  
Schmidtea mediterranea mk4.025092.00  
Schmidtea mediterranea mk4.012524.00  
Schmidtea mediterranea mk4.003933.01  
Schmidtea mediterranea mk4.082302.00  
Schmidtea mediterranea mk4.002789.03   Dual specificity mitogen-activated protein kinase kinase mek-2
Trypanosoma brucei gambiense Tbg972.10.6430   protein kinase, putative,MAP kinase kinase, putative
Trypanosoma brucei Tb927.10.5270   mitogen-activated protein kinase kinase 5
Trypanosoma congolense TcIL3000_10_4410   mitogen-activated protein kinase kinase 5
Trypanosoma cruzi TcCLB.506679.40   mitogen-activated protein kinase kinase 5
Trichomonas vaginalis TVAG_468570   STE family protein kinase
Trichomonas vaginalis TVAG_495060   STE family protein kinase
Trichomonas vaginalis TVAG_349370   STE family protein kinase

Essentiality

TcIL3000_10_4410 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.5270 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5270 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.5270 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.5270 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y54E10BL.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y54E10BL.6 Caenorhabditis elegans larval arrest wormbase
CELE_Y54E10BL.6 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus mitogen-activated protein kinase kinase 1 Compounds References
Homo sapiens mitogen-activated protein kinase kinase 2 Compounds References
Homo sapiens mitogen-activated protein kinase kinase 1 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 25.8% 283 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 24.1% 299 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 25.3% 300 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 24.9% 285 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 26.9% 286 aa Compounds References
Patiria pectinifera Cdc2 300 aa 26.6% 282 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 24.4% 295 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TcIL3000_10_4410 (Trypanosoma congolense), mitogen-activated protein kinase kinase 5
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