Detailed view for Tb927.2.4590

Basic information

TDR Targets ID: 55175
Trypanosoma brucei, branched-chain amino acid aminotransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.3274 | Length (AA): 367 | MW (Da): 40433 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01063   Amino-transferase class IV

Gene Ontology

Mouse over links to read term descriptions.
GO:0005739   mitochondrion  
GO:0004084   branched-chain-amino-acid transaminase activity  
GO:0003824   catalytic activity  
GO:0009082   branched chain family amino acid biosynthetic process  
GO:0009081   branched chain family amino acid metabolic process  
GO:0008152   metabolic process  
GO:0006520   amino acid metabolic process  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126731)

Species Accession Gene Product
Arabidopsis thaliana AT3G05190   branched-chain-amino-acid aminotransferase-like protein 1
Arabidopsis thaliana AT5G27410   branched-chain-amino-acid aminotransferase-like protein 2
Arabidopsis thaliana AT1G10070   branched-chain-amino-acid aminotransferase 2
Arabidopsis thaliana AT3G49680   branched-chain-amino-acid aminotransferase 3
Brugia malayi Bm1_37270   Branched-chain amino acid aminotransferase, cytosolic, putative
Candida albicans CaO19.8417   N terminus of likely Branched-chain Amino acid Transaminase CaP19.797
Candida albicans CaO19.8416   N terminus of likely Branched-chain Amino acid Transaminase CaP19.797
Candida albicans CaO19.797   similar to S. cerevisiae branched-chain amino acid transaminase BAT2 (YJR148W) expressed during stationary phase
Candida albicans CaO19.6994   Branched-chain amino acid transaminase
Caenorhabditis elegans CELE_K02A4.1   Protein BCAT-1
Caenorhabditis elegans CELE_Y44A6D.5   Protein Y44A6D.5
Dictyostelium discoideum DDB_G0285509   branched-chain amino acid aminotransferase
Drosophila melanogaster Dmel_CG1673   CG1673 gene product from transcript CG1673-RA
Escherichia coli b3770   branched-chain amino-acid aminotransferase
Entamoeba histolytica EHI_152310   branched-chain amino acid aminotransferase, putative
Giardia lamblia GL50803_6184   Branched-chain amino acid aminotransferase lateral transfer candidate
Homo sapiens ENSG00000105552   branched chain amino-acid transaminase 2, mitochondrial
Homo sapiens ENSG00000060982   branched chain amino-acid transaminase 1, cytosolic
Leishmania braziliensis LbrM.27.2210   branched-chain amino acid aminotransferase, putative
Leishmania infantum LinJ.27.1950   branched-chain amino acid aminotransferase, putative
Leishmania major LmjF.27.2030   branched-chain amino acid aminotransferase, putative
Leishmania mexicana LmxM.27.2032   branched-chain amino acid aminotransferase, putative
Leishmania mexicana LmxM.27.2030   branched-chain amino acid aminotransferase, putative
Loa Loa (eye worm) LOAG_04874   branched-chain amino acid aminotransferase
Mycobacterium leprae ML0866   PROBABLE BRANCHED-CHAIN AMINO ACID TRANSAMINASE ILVE
Mus musculus ENSMUSG00000030268   branched chain aminotransferase 1, cytosolic
Mus musculus ENSMUSG00000030826   branched chain aminotransferase 2, mitochondrial
Mycobacterium tuberculosis Rv2210c   Branched-chain amino acid transaminase IlvE
Mycobacterium ulcerans MUL_3570   branched-chain amino acid aminotransferase
Neospora caninum NCLIV_006760   Branched-chain-amino-acid aminotransferase (EC 2.6.1.42), related
Oryza sativa 4331322   Os03g0106400
Oryza sativa 9270815   Os03g0359100
Oryza sativa 4339583   Os05g0558400
Oryza sativa 4332151   Os03g0231600
Oryza sativa 4336645   Os04g0559400
Saccharomyces cerevisiae YJR148W   branched-chain-amino-acid transaminase BAT2
Saccharomyces cerevisiae YHR208W   branched-chain-amino-acid transaminase BAT1
Schmidtea mediterranea mk4.027344.01   Branched-chain-amino-acid aminotransferase
Schmidtea mediterranea mk4.001344.04   Branched-chain-amino-acid aminotransferase, cytosolic
Trypanosoma brucei gambiense Tbg.972.2.2670   branched-chain amino acid aminotransferase, putative
Trypanosoma brucei gambiense Tbg.972.2.2660   branched-chain amino acid aminotransferase, putative
Trypanosoma brucei Tb927.2.4590   branched-chain amino acid aminotransferase, putative
Trypanosoma brucei Tb927.2.4610   branched-chain amino acid aminotransferase, putative
Trypanosoma brucei Tb11.v5.0820   branched-chain amino acid aminotransferase, putative
Trypanosoma congolense TcIL3000_0_53430   branched-chain amino acid aminotransferase, putative
Toxoplasma gondii TGME49_297850   Branched-chain-amino-acid aminotransferase
Trichomonas vaginalis TVAG_026740   branched-chain amino acid aminotransferase, putative
Trichomonas vaginalis TVAG_139240   subgroup IIIi aminotransferase, putative

Essentiality

Tb927.2.4590 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2244 Mycobacterium tuberculosis essential nmpdr
Tb927.2.4590 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.4590 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.4590 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.2.4590 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3770 Escherichia coli non-essential goodall
CELE_K02A4.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_K02A4.1 Caenorhabditis elegans larval lethal wormbase
TGME49_297850 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens branched chain amino-acid transaminase 1, cytosolic Compounds References
Homo sapiens branched chain amino-acid transaminase 2, mitochondrial Compounds References
Rattus norvegicus Branched-chain-amino-acid transferase Compounds References
Rattus norvegicus Branched-chain-amino-acid transferase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0526 0.3241 1
0.023 0.5 0.5
0.0115 0.5 0.5
0.0115 0.5 0.5
0.0497 0.6149 1
0.0343 0.4028 1
0.0296 1 1
0.0093 0.3079 1
0.0388 1 0.5
0.1041 1 1
0.275 0.5 0.5
0.0096 0.5 0.5
0.0471 1 1
0.0408 0.4416 1
0.0115 0.5 0.5
0.01 0.5 0.5
0.0098 0.5 0.5
0.2052 0.5 0.5
0.1384 1 1
0.0268 0.4291 1
0.1931 1 1
0.0596 0.7371 1
0.1967 0.8084 1
0.0394 0.6121 1
0.0115 0.5 0.5
0.2776 1 1
0.0596 0.7289 1
0.0115 0.5 0.5
0.2162 0.5 0.5
0.0115 0.5 0.5
0.1428 0.5 0.5
0.0115 0.5 0.5
0.2287 0.5 0.5
0.0092 0.39 1
0.1633 0.5 0.5
0.0115 0.5 0.5
0.0193 0.408 0.408
0.1662 1 1
0.0096 0.5 0.5
0.0988 1 1
0.0385 1 1
0.0115 0.5 0.5
0.0497 0.6149 1
0.2185 0.5 0.5
0.0195 0.5 0.5
0.0101 0.5 0.5
0.1145 1 1
0.172 0.8703 0.8992
0.0209 0.2901 1
0.1428 0.5 0.5
0.0115 0.5 0.5
0.2476 1 1
0.2303 1 1
0.1422 1 1
0.0221 0.2824 1
0.1145 1 1
0.0596 0.7289 1
0.1758 0.5 0.5
0.023 0.5 0.5
0.0282 0.3262 1
0.1041 1 1
0.2169 1 1
0.0319 1 1
0.0221 0.2824 1
0.0874 1 1
0.1145 1 1
0.0436 0.2984 1
0.0115 0.5 0.5
0.0379 0.4653 1
0.2568 0.5 0.5
0.0115 0.5 0.5
0.0115 0.5 0.5
0.0203 0.3462 1
0.0286 0.4637 1
0.0115 0.5 0.5
0.1933 0.5 0.5
0.235 0.5 0.5
0.2166 1 1
0.2057 1 1
0.2182 1 1
0.0596 0.7371 1
0.0408 1 1
0.0596 0.7289 1
0.0202 0.5445 1
0.1145 1 1
0.062 0.7601 1
0.0497 0.6149 1
0.0115 0.5 0.5
0.2129 1 1
0.0376 0.4139 1
0.01 0.5 0.5
0.0093 0.3059 1
0.2531 0.5 0.5
0.0115 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.2.4590 (Trypanosoma brucei), branched-chain amino acid aminotransferase, putative
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