Detailed view for PF3D7_1250300

Basic information

TDR Targets ID: 5537
Plasmodium falciparum, vacuolar protein sorting-associated protein 26, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.5257 | Length (AA): 297 | MW (Da): 34509 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03643   Vacuolar protein sorting-associated protein 26

Gene Ontology

Mouse over links to read term descriptions.
GO:0003674   molecular_function  
GO:0042147   retrograde transport, endosome to Golgi  
GO:0030904   retromer complex  
GO:0007034   vacuolar transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 295 2fau (A) 7 298 53.00 0 1 1.53 -0.41
4 294 2fau (A) 6 297 52.00 0 1 1.5533 0.08
6 150 4r7x (A) 3 160 14.00 0 0.97 0.443215 0.22
9 294 2r51 (A) 9 295 54.00 0 1 1.59946 -0.15

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 40 hs, gametocyte, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, Sporozoite. Otto TD Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127354)

Species Accession Gene Product
Arabidopsis thaliana AT5G53530   vacuolar protein sorting 26A
Arabidopsis thaliana AT4G27690   vacuolar protein sorting-associated protein 26B
Babesia bovis BBOV_III008800   vacuolar protein sorting-associated protein 26 family protein
Brugia malayi Bm1_13115   vacuolar protein sorting 26 homolog
Candida albicans CaO19.6927   vesicular transport
Candida albicans CaO19.14189   vesicular transport
Caenorhabditis elegans CELE_T20D3.7   Protein VPS-26, isoform B
Cryptosporidium hominis Chro.20171   vps26 protein
Cryptosporidium parvum cgd2_1570   putative vacuolar protein sorting/targeting protein 26
Dictyostelium discoideum DDB_G0269168   vacuolar protein sorting-associated protein 26
Drosophila melanogaster Dmel_CG14804   Vacuolar protein sorting 26
Echinococcus granulosus EgrG_000123900   vacuolar protein sorting 26 vps26
Entamoeba histolytica EHI_062490   vacuolar sorting protein 26, putative
Entamoeba histolytica EHI_008470   vacuolar sorting protein 26, putative
Echinococcus multilocularis EmuJ_000123900   vacuolar protein sorting 26, vps26
Giardia lamblia GL50803_100864   Vacuolar protein sorting 26, putative
Homo sapiens ENSG00000151502   vacuolar protein sorting 26 homolog B (S. pombe)
Leishmania braziliensis LbrM.35.3650   vacuolar protein sorting-associated protein-like protein
Leishmania donovani LdBPK_363580.1   vacuolar protein sorting-associated protein-like protein
Leishmania infantum LinJ.36.3580   vacuolar protein sorting-associated protein-like protein
Leishmania major LmjF.36.3420   vacuolar protein sorting-associated protein-like protein
Leishmania mexicana LmxM.36.3420   vacuolar protein sorting-associated protein-like protein
Loa Loa (eye worm) LOAG_08902   hypothetical protein
Mus musculus ENSMUSG00000020078   vacuolar protein sorting 26 homolog A (yeast)
Mus musculus ENSMUSG00000031988   vacuolar protein sorting 26 homolog B (yeast)
Neospora caninum NCLIV_024440   hypothetical protein
Oryza sativa 4350961   Os11g0629200
Oryza sativa 4352271   Os12g0500700
Plasmodium berghei PBANKA_1463200   vacuolar protein sorting-associated protein 26, putative
Plasmodium falciparum PF3D7_1250300   vacuolar protein sorting-associated protein 26, putative
Plasmodium knowlesi PKNH_1470300   vacuolar protein sorting-associated protein 26, putative
Plasmodium vivax PVX_101410   vacuolar protein sorting-associated protein 26, putative
Plasmodium yoelii PY05367   vps26 protein homolog
Saccharomyces cerevisiae YJL053W   retromer subunit PEP8
Schistosoma japonicum Sjp_0077550   Vacuolar protein sorting-associated protein 26, putative
Schistosoma mansoni Smp_073130.3   vacuolar protein sorting 26 vps26
Schmidtea mediterranea mk4.007653.06  
Schmidtea mediterranea mk4.004709.02   Vacuolar protein sorting-associated protein 26
Schmidtea mediterranea mk4.007653.05   Vacuolar protein sorting-associated protein 26
Schmidtea mediterranea mk4.044752.00   Putative vacuolar protein sorting 26, vps26
Schmidtea mediterranea mk4.005966.00  
Schmidtea mediterranea mk4.001560.01   Putative vacuolar protein sorting 26, vps26
Trypanosoma brucei gambiense Tbg972.11.11240   vacuolar sorting-associated protein-like, putative
Trypanosoma brucei Tb927.11.10010   vacuolar sorting-associated protein-like, putative
Trypanosoma congolense TcIL3000.11.10530   vacuolar sorting-associated protein-like, putative
Trypanosoma congolense TcIL3000_0_45560   vacuolar sorting-associated protein-like, putative
Trypanosoma cruzi TcCLB.510719.10   vacuolar protein sorting-associated protein-like, putative
Trypanosoma cruzi TcCLB.506337.10   vacuolar protein sorting-associated protein-like, putative
Toxoplasma gondii TGME49_263500   vacuolar protein sorting-associated protein 26, putative
Theileria parva TP04_0783   vacuolar sorting protein 26, putative
Trichomonas vaginalis TVAG_177370   vacuolar protein sorting 26, vps26, putative
Trichomonas vaginalis TVAG_106660   vacuolar protein sorting 26, vps26, putative
Trichomonas vaginalis TVAG_160820   vacuolar protein sorting 26, vps26, putative

Essentiality

PF3D7_1250300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.1770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.1770 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.1770 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T20D3.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_T20D3.7 Caenorhabditis elegans larval arrest wormbase
CELE_T20D3.7 Caenorhabditis elegans sterile wormbase
PBANKA_1463200 Plasmodium berghei Essential plasmo
TGME49_263500 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_1250300 (Plasmodium falciparum), vacuolar protein sorting-associated protein 26, putative
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