Detailed view for Rv3391

Basic information

TDR Targets ID: 5633
Mycobacterium tuberculosis, Possible multi-functional enzyme with acyl-CoA-reductase activity AcrA1

Source Database / ID:  Tuberculist 

pI: 10.1097 | Length (AA): 650 | MW (Da): 70940 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00106   short chain dehydrogenase
PF07993   Male sterility protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0016491   oxidoreductase activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 252 1kew (A) 1 275 24.00 0.00000016 0.91 0.366092 0.3
2 322 4w4t (A) 1150 1489 28.00 0 1 0.744246 -0.23
4 100 4g5h (A) 8 111 26.00 0.13 0.55 0.260631 -0.49
34 107 5lqd (A) 238 325 39.00 0.42 0.97 0.299246 0.29
155 599 2fr0 (A) 1453 1910 19.00 0 1 0.722515 0.62
185 624 3mje (A) 64 467 18.00 0 1 0.663823 0.73
349 540 1cyd (A) 4 186 28.00 0 1 0.664785 -0.72
352 616 1xhl (A) 6 270 30.00 0 1 0.740192 -0.14
352 543 1vl8 (A) 9 200 26.00 0 1 0.710785 -0.98
352 628 5jy1 (A) 6 265 28.00 0 1 0.758554 -0.45
466 513 1hds (B) 26 70 44.00 0.58 0.4 0.400346 0.82

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_130166)

Species Accession Gene Product
Brugia malayi Bm1_09265   Dehydrogenases, short chain protein 30
Caenorhabditis elegans CELE_T25G12.7   Protein DHS-30
Caenorhabditis elegans CELE_T25G12.13   Protein T25G12.13
Caenorhabditis elegans CELE_T25G12.2   Protein T25G12.2
Drosophila melanogaster Dmel_CG7601   CG7601 gene product from transcript CG7601-RA
Giardia lamblia GL50803_8382   Short-chain dehydrogenase, putative
Homo sapiens ENSG00000109016   dehydrogenase/reductase (SDR family) member 7B
Leishmania braziliensis LbrM.35.3640   short chain dehydrogenase-like protein
Leishmania donovani LdBPK_363570.1   short chain dehydrogenase-like protein
Leishmania infantum LinJ.36.3570   short chain dehydrogenase-like protein
Leishmania major LmjF.36.3410   short chain dehydrogenase-like protein
Leishmania mexicana LmxM.36.3410   short chain dehydrogenase-like protein
Loa Loa (eye worm) LOAG_13677   dehydrogenase
Loa Loa (eye worm) LOAG_12558   hypothetical protein
Mus musculus ENSMUSG00000042569   dehydrogenase/reductase (SDR family) member 7B
Mycobacterium tuberculosis Rv3391   Possible multi-functional enzyme with acyl-CoA-reductase activity AcrA1
Mycobacterium tuberculosis Rv0945   Probable short-chain type dehydrogenase/reductase
Mycobacterium tuberculosis Rv1050   Probable oxidoreductase
Mycobacterium ulcerans MUL_4417   short chain dehydrogenase
Mycobacterium ulcerans MUL_4346   multi-functional enzyme with acyl-CoA-reductase activity AcrA1
Mycobacterium ulcerans MUL_0918   short chain dehydrogenase
Mycobacterium ulcerans MUL_3139   short-chain dehydrogenase
Oryza sativa 4329454   Os02g0511400
Oryza sativa 4350557   Os11g0499600
Schmidtea mediterranea mk4.005667.01  
Schmidtea mediterranea mk4.010259.00  
Trypanosoma brucei gambiense Tbg972.11.11250   short-chain dehydrogenase, putative
Trypanosoma brucei Tb927.11.10020   short-chain dehydrogenase, putative
Trypanosoma congolense TcIL3000_0_45550   short-chain dehydrogenase, putative
Trypanosoma congolense TcIL3000.11.10540   short-chain dehydrogenase, putative
Trypanosoma cruzi TcCLB.509749.39   short-chain dehydrogenase, putative
Trypanosoma cruzi TcCLB.510719.20   short-chain dehydrogenase, putative

Essentiality

Rv3391 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3451 this record Mycobacterium tuberculosis non-essential nmpdr
Tb11.01.1780 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.1780 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.1780 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.1780 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Macaca mulatta Hydroxysteroid 11-beta dehydrogenase 1 140 aa 32.6% 141 aa Compounds References
Canis lupus familiaris 11-beta-hydroxysteroid dehydrogenase type 1 293 aa 29.2% 288 aa Compounds References
Rattus norvegicus 11-beta-hydroxysteroid dehydrogenase 1 288 aa 28.4% 232 aa Compounds References

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0009 0.5 0.5
0.0005 0.5 0.5
0.007 0.2916 1
0.0012 0.5 0.5
0.0058 1 1
0.0066 0.3586 0.5
0.0053 1 1
0.008 0.5 0.5
0.0123 0.5 0.5
0.0012 0.5 0.5
0.0056 1 0.5
0.007 1 0.5
0.0165 1 0.5
0.0082 1 0.5
0.0004 0.5 0.5
0.0076 1 0.5
0.007 0.2916 1
0.0016 0.5 0.5
0.0021 0.3321 0.5
0.0132 0.3658 0.376
0.0009 1 0.5
0.0071 0.3141 0
0.0009 0.5 0.5
0.0085 1 0.5
0.0131 0.5 0.5
0.0088 0.6544 0.5
0.0046 0.3082 0.5
0.0402 1 1
0.0068 0.2585 0.5
0.0106 1 1
0.0106 0.2872 0.4772
0.0004 0.5 0.5
0.0009 0.5 0.5
0.0162 1 0.5
0.0171 0.4063 0.5
0.004 0.5 0.5
0.0004 0.5 0.5
0.0009 0.5 0.5
0.0045 1 0.5
0.0113 1 1
0.0097 1 1
0.016 0.577 1
0.0408 0.5144 0.5
0.0019 0.4322 0.5
0.0053 1 0.5
0.0009 0.7374 0.5
0.0196 0.8679 0.5
0.011 0.3568 0.3299
0.0039 1 0.5
0.0073 0.3141 0.4101
0.0058 0.3931 1
0.007 1 0.5
0.0068 1 0.5
0.0196 0.8679 0.5
0.0009 0.5 0.5
0.0073 0.3811 1
0.0171 0.4063 0.5
0.0089 0.5 0.5
0.0093 0.5 0.5
0.0021 0.3321 0.5
0.0032 1 1
0.0027 0.5 0.5
0.0057 0.284 0.6413
0.0013 1 0.5
0.0087 0.5 0.5
0.0222 0.5 0.5
0.0106 1 0.5
0.0027 0.5 0.5
0.0004 0.5 0.5
0.0004 0.5 0.5
0.0157 0.5 0.5
0.0114 1 0.5
0.007 0.2916 1
0.0196 0.8679 0.5
0.0088 1 0.5
0.0013 0.5 0.5
0.0108 0.5 0.5
0.0114 1 0.5
0.007 1 0.5
0.016 0.577 1
0.0073 0.2873 0.3563
0.0009 0.5 0.5
0.0073 0.4546 1
0.0106 0.5 0.5
0.0056 0.875 1
0.0218 0.5 0.5
0.0004 0.5 0.5
0.0026 0.5 0.5
0.0009 0.5 0.5
0.0004 0.5 0.5
0.0102 0.7451 0.5
0.0118 1 0.5
0.0073 0.3811 1
0.0064 0.3508 0.5
0.0067 0.3468 0.5
0.0097 0.4304 0.5
0.0408 0.5144 0.5
0.0035 1 0.5
0.0005 0.5 0.5
0.0084 0.4283 1
0.0021 0.5 0.5
0.0119 0.2746 0
0.0089 0.5 0.5
0.0408 0.5144 0.5
0.0068 1 0.5
0.0009 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

9 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Rv3391 (Mycobacterium tuberculosis), Possible multi-functional enzyme with acyl-CoA-reductase activity AcrA1
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