Detailed view for Rv2780

Basic information

TDR Targets ID: 5650
Mycobacterium tuberculosis, Secreted L-alanine dehydrogenase Ald (40 kDa antigen) (TB43)

Source Database / ID:  Tuberculist 

pI: 6.17 | Length (AA): 371 | MW (Da): 38713 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01262   Alanine dehydrogenase/PNT, C-terminal domain
PF05222   Alanine dehydrogenase/PNT, N-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0042853   L-alanine catabolic process  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0000286   alanine dehydrogenase activity  
GO:0016491   oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 371 4lmp (A) 1 371 99.99 0 1 2.2075 -1.23

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

  • 2VHV:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 2VHW:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 2VHX:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 2VHY:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 2VHZ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 2VOE:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 2VOJ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 4LMP:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127241)

Species Accession Gene Product
Caenorhabditis elegans CELE_C15H9.1   Protein NNT-1
Cryptosporidium hominis Chro.10120   NAD(P) transhydrogenase beta subunit
Cryptosporidium hominis Chro.80274   transhydrogenase 7B2
Cryptosporidium parvum cgd1_990   pyridine nucleotide/ NAD(P) transhydrogenase alpha plus beta subunits, duplicated gene, 12 transmembrane domain
Cryptosporidium parvum cgd8_2330   pyridine nucleotide/ NAD(P) transhydrogenase alpha plus beta subunits, duplicated gene, possible signal peptide plus 12 transmem
Dictyostelium discoideum DDB_G0269046   NAD+ transhydrogenase
Escherichia coli b1603   pyridine nucleotide transhydrogenase, alpha subunit
Escherichia coli b1602   pyridine nucleotide transhydrogenase, beta subunit
Echinococcus granulosus EgrG_000591000   nadp transhydrogenase
Entamoeba histolytica EHI_055400   NAD(P)( ) transhydrogenase (AB-specific), alpha subunit, putative
Entamoeba histolytica EHI_014030   NAD(P) transhydrogenase subunit alpha, putative
Entamoeba histolytica EHI_060020   NAD(P) transhydrogenase beta subunit, putative
Echinococcus multilocularis EmuJ_000591000   nadp transhydrogenase
Homo sapiens ENSG00000112992   nicotinamide nucleotide transhydrogenase
Mycobacterium leprae ML1532   SECRETED L-ALANINE DEHYDROGENASE ALD (40 KDA ANTIGEN) (TB43)
Mycobacterium leprae ML2636c   PROBABLE NAD(P) TRANSHYDROGENASE (SUBUNIT ALPHA) PNTAA [FIRST PART; CATALYTIC PART] (PYRIDINE NUCLEOTIDE TRANSHYDROGENASE SUBUNI
Mycobacterium leprae ML2634c   PROBABLE NAD(P) TRANSHYDROGENASE (SUBUNIT BETA) PNTB [INTEGRAL MEMBRANE PROTEIN] (PYRIDINE NUCLEOTIDE TRANSHYDROGENASE SUBUNIT B
Mus musculus ENSMUSG00000025453   nicotinamide nucleotide transhydrogenase
Mycobacterium tuberculosis Rv2780   Secreted L-alanine dehydrogenase Ald (40 kDa antigen) (TB43)
Mycobacterium tuberculosis Rv0155   Probable NAD(P) transhydrogenase (subunit alpha) PntAa [first part; catalytic part] (pyridine nucleotide transhydrogenase subuni
Mycobacterium tuberculosis Rv0157   Probable NAD(P) transhydrogenase (subunit beta) PntB [integral membrane protein] (pyridine nucleotide transhydrogenase subunit b
Mycobacterium ulcerans MUL_2154   secreted L-alanine dehydrogenase Ald (40 kDa antigen)
Mycobacterium ulcerans MUL_4735   NAD(P) transhydrogenase PntA
Mycobacterium ulcerans MUL_4733   NAD(P) transhydrogenasePntB
Neospora caninum NCLIV_012720   NAD(P) transhydrogenase, beta subunit, related
Neospora caninum NCLIV_058000   alanine dehydrogenase, putative
Plasmodium berghei PBANKA_1317200   pyridine nucleotide transhydrogenase, putative
Plasmodium falciparum PF3D7_1453500   pyridine nucleotide transhydrogenase, putative
Plasmodium knowlesi PKNH_1228600   pyridine nucleotide transhydrogenase, putative
Plasmodium vivax PVX_117805   pyridine nucleotide transhydrogenase, putative
Plasmodium yoelii PY05907   NAD(P) transhydrogenase beta subunit, putative
Schistosoma japonicum Sjp_0011150   ko:K00324 NAD(P) transhydrogenase subunit alpha [EC1.6.1.2A], putative
Schistosoma japonicum Sjp_0134090   NAD(P) transhydrogenase subunit alpha, putative
Schistosoma mansoni Smp_082370   NADP transhydrogenase
Schmidtea mediterranea mk4.006034.00  
Schmidtea mediterranea mk4.003173.00   NAD(P) transhydrogenase, mitochondrial
Schmidtea mediterranea mk4.011090.00   NAD(P) transhydrogenase, mitochondrial
Schmidtea mediterranea mk4.011090.03  
Schmidtea mediterranea mk4.006604.00   NAD(P) transhydrogenase, mitochondrial
Toxoplasma gondii TGME49_315260   alanine dehydrogenase
Toxoplasma gondii TGME49_301210   NAD(P) transhydrogenase subunit beta, putative
Trichomonas vaginalis TVAG_445130   nADP transhydrogenase, putative
Trichomonas vaginalis TVAG_235800   nADP transhydrogenase, putative

Essentiality

Rv2780 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2827 this record Mycobacterium tuberculosis non-essential nmpdr
mtu158 Mycobacterium tuberculosis non-essential nmpdr
b1602 Escherichia coli non-essential goodall
b1603 Escherichia coli non-essential goodall
PBANKA_1317200 Plasmodium berghei Dispensable plasmo
TGME49_301210 Toxoplasma gondii Essentiality uncertain sidik
TGME49_315260 Toxoplasma gondii Essentiality uncertain sidik
TGME49_301210 Toxoplasma gondii Probably non-essential sidik
TGME49_315260 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for L-Alanine Dehydrogenase (1.4.1.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

18 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2780 (Mycobacterium tuberculosis), Secreted L-alanine dehydrogenase Ald (40 kDa antigen) (TB43)
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