Detailed view for Rv0032

Basic information

TDR Targets ID: 5717
Mycobacterium tuberculosis, Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synth
Source Database / ID:  Tuberculist 

pI: 7.0007 | Length (AA): 771 | MW (Da): 86243 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00155   Aminotransferase class I and II
PF13480   Acetyltransferase (GNAT) domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0030170   pyridoxal phosphate binding  
GO:0016740   transferase activity  
GO:0003824   catalytic activity  
GO:0009058   biosynthetic process  
GO:0008152   metabolic process  

Metabolic Pathways

Biotin metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
360 771 3mad (A) 63 475 16.00 0 1 0.513171 0.79
377 762 3a2b (A) 6 391 37.00 0 1 0.989249 -0.56
465 516 4lnj (A) 38 89 38.00 0.18 0.31 0.307845 1.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_126783)

Species Accession Gene Product
Arabidopsis thaliana AT5G04620   7-keto-8-aminopelargonic acid (KAPA) synthase
Brugia malayi Bm1_01535   2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor
Candida albicans CaO19.2601   similar to S. cerevisiae HEM1 (YDR232W) mitochondrial 5-aminolevulinic acid synthase involved in heme biosynthesis
Candida albicans CaO19.10132   similar to S. cerevisiae HEM1 (YDR232W) mitochondrial 5-aminolevulinic acid synthase involved in heme biosynthesis
Caenorhabditis elegans CELE_T25B9.1   Protein T25B9.1
Dictyostelium discoideum DDB_G0280763   5-aminolevulinate synthase
Drosophila melanogaster Dmel_CG3017   Aminolevulinate synthase
Drosophila melanogaster Dmel_CG10361   CG10361 gene product from transcript CG10361-RA
Escherichia coli b0776   8-amino-7-oxononanoate synthase
Escherichia coli b3617   glycine C-acetyltransferase
Echinococcus granulosus EgrG_000107200   2 amino 3 ketobutyrate coenzyme A ligase
Echinococcus granulosus EgrG_000574600   5 aminolevulinate synthase
Echinococcus granulosus EgrG_000575200   5 aminolevulinate synthase
Echinococcus multilocularis EmuJ_000107200   2 amino 3 ketobutyrate coenzyme A ligase
Echinococcus multilocularis EmuJ_000574600   5 aminolevulinate synthase
Echinococcus multilocularis EmuJ_000575200   5 aminolevulinate synthase
Homo sapiens ENSG00000158578   5'-aminolevulinate synthase 2
Homo sapiens ENSG00000023330   5'-aminolevulinate synthase 1
Homo sapiens ENSG00000100116   glycine C-acetyltransferase
Loa Loa (eye worm) LOAG_04085   2-amino-3-ketobutyrate coenzyme A ligase
Mycobacterium leprae ML1217   PROBABLE 8-AMINO-7-OXONONANOATE SYNTHASE BIOF (AONS) (8-AMINO-7-KETOPELARGONATE SYNTHASE) (7-KETO-8-AMINO-PELARGONIC ACID SYNTHE
Mus musculus ENSMUSG00000006378   glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase)
Mus musculus ENSMUSG00000025270   aminolevulinic acid synthase 2, erythroid
Mus musculus ENSMUSG00000032786   aminolevulinic acid synthase 1
Mycobacterium tuberculosis Rv1569   Probable 8-amino-7-oxononanoate synthase BioF1 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synth
Mycobacterium tuberculosis Rv0032   Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synth
Mycobacterium ulcerans MUL_4045   8-amino-7-oxononanoate synthase
Mycobacterium ulcerans MUL_1560   8-amino-7-oxononanoate synthase
Mycobacterium ulcerans MUL_0241   8-amino-7-oxononanoate synthase
Mycobacterium ulcerans MUL_1966   8-amino-7-oxononanoate synthase
Mycobacterium ulcerans MUL_2843   8-amino-7-oxononanoate synthase
Neospora caninum NCLIV_027820   5-aminolevulinic acid synthase, related
Oryza sativa 4349380   Os10g0560900
Oryza sativa 4327870   Os01g0736400
Plasmodium berghei PBANKA_1459200   delta-aminolevulinic acid synthetase
Plasmodium falciparum PF3D7_1246100   delta-aminolevulinic acid synthetase
Plasmodium knowlesi PKNH_1465700   delta-aminolevulinic acid synthetase, putative
Plasmodium vivax PVX_101195   delta-aminolevulinic acid synthetase, putative
Plasmodium yoelii PY05459   delta-aminolevulinic acid synthetase
Saccharomyces cerevisiae YDR232W   5-aminolevulinate synthase
Schistosoma japonicum Sjp_0209020   ko:K00643 5-aminolevulinate synthase [EC2.3.1.37], putative
Schistosoma mansoni Smp_045260   5-aminolevulinic acid synthase
Schmidtea mediterranea mk4.000755.02   2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
Schmidtea mediterranea mk4.052690.00   2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
Schmidtea mediterranea mk4.042742.00   2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
Schmidtea mediterranea mk4.042742.01   2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
Schmidtea mediterranea mk4.000655.01   5-aminolevulinate synthase, nonspecific, mitochondrial
Trypanosoma brucei gambiense Tbg972.8.6090   2-amino-3-ketobutyrate coenzyme A ligase, putative,glycine acetyltransferase, putative
Trypanosoma brucei Tb927.8.6060   glycine acetyltransferase, putative
Trypanosoma congolense TcIL3000_8_5850   glycine acetyltransferase, putative
Trypanosoma cruzi TcCLB.511899.40   2-amino-3-ketobutyrate coenzyme A ligase, putative
Trypanosoma cruzi TcCLB.511899.10   2-amino-3-ketobutyrate coenzyme A ligase, putative
Trypanosoma cruzi TcCLB.511071.140   2-amino-3-ketobutyrate coenzyme A ligase, putative
Toxoplasma gondii TGME49_258690   5-aminolevulinic acid synthase domain-containing protein
Wolbachia endosymbiont of Brugia malayi Wbm0133   5-aminolevulinate synthase

Essentiality

Rv0032 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1597 Mycobacterium tuberculosis non-essential nmpdr
mtu32 this record Mycobacterium tuberculosis non-essential nmpdr
Tb927.8.6060 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.6060 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.6060 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.6060 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0776 Escherichia coli non-essential goodall
b3617 Escherichia coli non-essential goodall
YDR232W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1459200 Plasmodium berghei Dispensable plasmo
TGME49_258690 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum delta-aminolevulinic acid synthetase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0032 (Mycobacterium tuberculosis), Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synth
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