Detailed view for Rv2335

Basic information

TDR Targets ID: 5836
Mycobacterium tuberculosis, Probable serine acetyltransferase CysE (sat)
Source Database / ID:  Tuberculist 

pI: 7.5604 | Length (AA): 229 | MW (Da): 23770 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00132   Bacterial transferase hexapeptide (six repeats)
PF14602  

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0016740   transferase activity  
GO:0009001   serine O-acetyltransferase activity  
GO:0006535   cysteine biosynthetic process from serine  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 183 3gvd (A) 77 260 42.00 0 1 1.40123 -0.89
1 168 4n6a (A) 88 255 50.00 0 1 1.42272 -0.96
56 170 3r8y (A) 106 218 35.00 0 1 1.05728 -1.22
122 153 4ihf (A) 114 145 34.00 0.0036 0.98 0.525838 0.45

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_128713)

Species Accession Gene Product
Arabidopsis thaliana AT3G13110   2
Arabidopsis thaliana AT4G35640   2
Arabidopsis thaliana AT1G55920   1
Arabidopsis thaliana AT5G56760   1
Arabidopsis thaliana AT2G17640   1
Escherichia coli b3607   serine acetyltransferase
Entamoeba histolytica EHI_153430   serine acetyltransferase 1
Entamoeba histolytica EHI_021570   serine acetyltransferase 1
Entamoeba histolytica EHI_202040   serine acetyltransferase, putative
Leishmania donovani LdBPK_342710.1   serine acetyltransferase
Leishmania infantum LinJ.34.2710   serine acetyltransferase
Leishmania major LmjF.34.2850   serine acetyltransferase
Leishmania mexicana LmxM.33.2850   serine acetyltransferase, putative
Mycobacterium leprae ML0838c   PROBABLE SERINE ACETYLTRANSFERASE CYSE (SAT)
Mycobacterium tuberculosis Rv2335   Probable serine acetyltransferase CysE (sat)
Mycobacterium ulcerans MUL_1464   serine acetyltransferase CysE
Oryza sativa 4324655   Os01g0720700
Oryza sativa 4331941   Os03g0196600
Oryza sativa 4331528   Os03g0133900
Oryza sativa 4339425   Os05g0533500
Oryza sativa 4331861   Os03g0185000
Trypanosoma cruzi TcCLB.510879.80   serine acetyltransferase, putative
Trypanosoma cruzi TcCLB.504013.40   serine acetyltransferase, putative

Essentiality

Rv2335 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2376 this record Mycobacterium tuberculosis non-essential nmpdr
b3607 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2335 (Mycobacterium tuberculosis), Probable serine acetyltransferase CysE (sat)
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