Detailed view for Rv2364c

Basic information

TDR Targets ID: 5940
Mycobacterium tuberculosis, Probable GTP-binding protein Era

Source Database / ID:  Tuberculist 

pI: 5.979 | Length (AA): 300 | MW (Da): 32460 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01926   50S ribosome-binding GTPase
PF07650   KH domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  
GO:0003723   RNA binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 300 1wf3 (A) 4 296 40.00 0 1 1.57113 -1.11
4 300 3iev (A) 1 299 37.00 0 1 1.5458 -1.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127948)

Species Accession Gene Product
Arabidopsis thaliana AT1G30960   GTP-binding family protein
Arabidopsis thaliana AT5G66470   GTP-binding protein Era-like protein
Babesia bovis BBOV_III008850   small GTP-binding protein domain containing protein
Brugia malayi Bm1_20700   Hypothetical GTP-binding protein E02H1.2 in chromosome II
Caenorhabditis elegans CELE_E02H1.2   Protein E02H1.2
Dictyostelium discoideum DDB_G0288609   hypothetical protein
Drosophila melanogaster Dmel_CG7488   CG7488 gene product from transcript CG7488-RA
Escherichia coli b2566   membrane-associated, 16S rRNA-binding GTPase
Echinococcus granulosus EgrG_000482150   Era-like GTP-binding protein.
Echinococcus multilocularis EmuJ_000482150   Era-like GTP-binding protein.
Homo sapiens ENSG00000132591   Era-like 12S mitochondrial rRNA chaperone 1
Leishmania braziliensis LbrM.25.0400   hypothetical protein, conserved
Leishmania donovani LdBPK_250460.1   50S ribosome-binding GTPase, putative
Leishmania infantum LinJ.25.0460   hypothetical protein, conserved
Leishmania major LmjF.25.0450   hypothetical protein, conserved
Leishmania mexicana LmxM.25.0450   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_03726   hypothetical protein
Mycobacterium leprae ML0631   Probable GTP-binding protein Era
Mus musculus ENSMUSG00000020832   Era (G-protein)-like 1 (E. coli)
Mycobacterium tuberculosis Rv2364c   Probable GTP-binding protein Era
Mycobacterium ulcerans MUL_3617   GTP-binding protein Era
Neospora caninum NCLIV_014010   hypothetical protein, conserved
Oryza sativa 9271571   Os05g0567300
Plasmodium berghei PBANKA_1009000   GTPase Era, putative
Plasmodium falciparum PF3D7_1435800   GTPase Era, putative
Plasmodium knowlesi PKNH_0422400   GTPase Era, putative
Plasmodium vivax PVX_084745   GTPase Era, putative
Plasmodium yoelii PY00616   GTP-binding protein Era
Schistosoma japonicum Sjp_0312390   ko:K03595 GTP-binding protein Era, putative
Schistosoma japonicum Sjp_0305540   IPR009019,KH, prokaryotic type,domain-containing
Schistosoma japonicum Sjp_0114700   expressed protein
Schistosoma mansoni Smp_164220   GTP-binding protein era
Schmidtea mediterranea mk4.063273.01   GTP-binding protein era, putative
Schmidtea mediterranea mk4.002727.04   GTP-binding protein era, putative
Trypanosoma brucei gambiense Tbg972.11.390   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.480   small GTP-binding protein domain containing protein, putative
Trypanosoma congolense TcIL3000.11.420   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507093.184   small GTP-binding protein domain containing protein, putative
Toxoplasma gondii TGME49_286640   GTPase
Treponema pallidum TP0541   GTP-binding protein Era
Theileria parva TP04_0788   hypothetical protein, conserved
Wolbachia endosymbiont of Brugia malayi Wbm0007   GTP-binding protein Era

Essentiality

Rv2364c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2405 this record Mycobacterium tuberculosis essential nmpdr
Tb11.03.0660 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.03.0660 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.03.0660 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.03.0660 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2566 Escherichia coli essential goodall
TGME49_286640 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Rv2364c (Mycobacterium tuberculosis), Probable GTP-binding protein Era
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