Detailed view for PF3D7_0718400

Basic information

TDR Targets ID: 595
Plasmodium falciparum, mitochondrial ribosomal protein S8 precursor, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 10.5861 | Length (AA): 129 | MW (Da): 14803 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00410   Ribosomal protein S8

Gene Ontology

Mouse over links to read term descriptions.
GO:0005763   mitochondrial small ribosomal subunit  
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 121 1i94 (H) 2 137 26.00 0 1 1.16 -0.99
2 121 1s03 (H) 3 128 28.00 0 1 1.37 -1.29
1 121 1i6u (A) 7 129 21.00 0 1 1.17848 -0.22
2 122 1s03 (H) 3 129 28.00 0 1 1.30178 -0.8
28 118 4kzy (W) 35 126 31.00 0.64 0.96 0.837026 0.67

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, sporozoite, early schizont, early trophozoite, late ring, late schizont, late trophozoite, Oocyst, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, merozoite, early ring, Ring, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile gametocyte. PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Female Gametocyte. Lasonder E
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_130342)

Species Accession Gene Product
Arabidopsis thaliana ArthCp058   30S ribosomal protein S8
Babesia bovis BBOV_IV006540   30S ribosomal protein S8, putative
Chlamydia trachomatis CT_515   30S ribosomal protein S8
Escherichia coli b3306   30S ribosomal subunit protein S8
Mycobacterium leprae ML1845c   PROBABLE 30S RIBOSOMAL PROTEIN S8 RPSH
Mycobacterium tuberculosis Rv0718   30S ribosomal protein S8 RpsH
Mycobacterium ulcerans MUL_0808   30S ribosomal protein S8
Neospora caninum NCLIV_026110   30S ribosomal protein S8, putative
Oryza sativa 3131446   30S ribosomal protein S8
Plasmodium berghei PBANKA_0616000   mitochondrial ribosomal protein S8 precursor, putative
Plasmodium falciparum PF3D7_0718400   mitochondrial ribosomal protein S8 precursor, putative
Plasmodium knowlesi PKNH_0313700   mitochondrial ribosomal protein S8 precursor, putative
Plasmodium vivax PVX_096405   30S ribosomal protein S8, putative
Plasmodium yoelii PY05271   ribosomal protein S8
Toxoplasma gondii TGME49_261380   ribosomal protein S8, putative
Treponema pallidum TP0203   30S ribosomal protein S8
Theileria parva TP01_1203   40S ribosomal protein S8, putative
Wolbachia endosymbiont of Brugia malayi Wbm0327   30S ribosomal protein S8

Essentiality

PF3D7_0718400 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu730 Mycobacterium tuberculosis essential nmpdr
b3306 Escherichia coli essential goodall
PBANKA_0616000 Plasmodium berghei Dispensable plasmo
TGME49_261380 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_0718400 (Plasmodium falciparum), mitochondrial ribosomal protein S8 precursor, putative
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