Detailed view for Rv0400c

Basic information

TDR Targets ID: 6053
Mycobacterium tuberculosis, Acyl-CoA dehydrogenase FadE7

Source Database / ID: Tuberculist / Rv0400c 

pI: 6.6521 | Length (AA): 395 | MW (Da): 42297 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Pfam domains

PF00441   Acyl-CoA dehydrogenase, C-terminal domain
PF02770   Acyl-CoA dehydrogenase, middle domain
PF02771   Acyl-CoA dehydrogenase, N-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016627   oxidoreductase activity, acting on the CH-CH group of donors  
GO:0003995   acyl-CoA dehydrogenase activity  
GO:0050660   FAD binding  
GO:0016491   oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 395 3swo (A) 10 396 63.00 0 1 1.80987 -1.28
11 395 3sf6 (A) 16 399 70.00 0 1 1.87448 -1.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein


Upregulation Percent Ranking Shows this level of upregulation in
This gene ranks in the upper 60-80% group of genes. Dormant phase.
Upregulation Percent Ranking Shows this level of upregulation in
This gene ranks in the lower 20-40% group of genes. Dormant phase.
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.


Ortholog group members (OG5_129299)

Species Accession Gene Product
Arabidopsis thaliana AT3G51840   acyl-coenzyme A oxidase 4
Caenorhabditis elegans CELE_F54D5.7   Protein F54D5.7
Dictyostelium discoideum DDB_G0283411   glutaryl-CoA dehydrogenase
Dictyostelium discoideum DDB_G0274585   hypothetical protein
Drosophila melanogaster Dmel_CG9547   CG9547 gene product from transcript CG9547-RA
Homo sapiens ENSG00000105607   glutaryl-CoA dehydrogenase
Mus musculus ENSMUSG00000003809   glutaryl-Coenzyme A dehydrogenase
Mycobacterium tuberculosis Rv0400c this record   Acyl-CoA dehydrogenase FadE7
Mycobacterium ulcerans MUL_2825   acyl-CoA dehydrogenase FadE7
Oryza sativa 4324062   Os01g0159400
Oryza sativa 4337904   Os05g0163700
Schmidtea mediterranea mk4.008512.01  
Schmidtea mediterranea mk4.008440.00  
Schmidtea mediterranea mk4.002644.03   Glutaryl-CoA dehydrogenase, mitochondrial
Schmidtea mediterranea mk4.022596.00  
Schmidtea mediterranea mk4.035706.00   Probable glutaryl-CoA dehydrogenase, mitochondrial
Trypanosoma cruzi TcCLB.510121.40   hypothetical protein
Trypanosoma cruzi TcCLB.508951.40   acyl-CoA dehydrogenase, putative
Toxoplasma gondii TGME49_214400   hypothetical protein
Toxoplasma gondii TGME49_231900   acyl-CoA dehydrogenase domain-containing protein


Rv0400c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu403 this record Mycobacterium tuberculosis essential nmpdr
TGME49_214400 Toxoplasma gondii Probably non-essential sidik
TGME49_231900 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site,, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please log in using your TDR Targets username and password and send them to us using the corresponding form in this page (only visible to registered users) or contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model


Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Rv0400c (Mycobacterium tuberculosis), Acyl-CoA dehydrogenase FadE7
Title for this comment