Detailed view for Rv2564

Basic information

TDR Targets ID: 6156
Mycobacterium tuberculosis, Probable glutamine-transport ATP-binding protein ABC transporter GlnQ
Source Database / ID:  Tuberculist 

pI: 5.2622 | Length (AA): 330 | MW (Da): 35429 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00005   ABC transporter
PF00027   Cyclic nucleotide-binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005952   cAMP-dependent protein kinase complex  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  
GO:0017111   nucleoside-triphosphatase activity  
GO:0016887   ATPase activity  
GO:0008603   cAMP-dependent protein kinase regulator activity  
GO:0001932   regulation of protein amino acid phosphorylation  

Metabolic Pathways

ABC transporters (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 330 4tqu (S) 2 314 28.00 0 1 1.1173 0.94
3 235 4u00 (A) 4 231 39.00 0 1 1.21636 -0.59
4 225 3tif (A) 2 228 39.00 0 1 1.24203 -1.08
237 317 3gyd (A) 54 134 20.00 0.00007 0.1 0.442755 0.04

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase. hasan
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_126906)

Species Accession Gene Product
Chlamydia trachomatis CT_152   ABC transporter ATPase
Dictyostelium discoideum DDB_G0267428   hypothetical protein
Dictyostelium discoideum DDB_G0292384   hypothetical protein
Escherichia coli b0495   putative ABC superfamily transporter ATP-binding subunit
Escherichia coli b0879   fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component
Escherichia coli b1117   outer membrane-specific lipoprotein transporter subunit
Entamoeba histolytica EHI_114390   ABC transporter, putative
Entamoeba histolytica EHI_099870   ATP-binding cassette, putative
Leishmania braziliensis LbrM.29.1750   ABC transporter domain protein, putative
Leishmania braziliensis LbrM.19.0280   ABC transporter
Leishmania donovani LdBPK_291780.1   ATP-binding cassette protein subfamily H, member 2, putative
Leishmania donovani LdBPK_110040.1   ATP-binding cassette protein subfamily H, member 1, putative
Leishmania infantum LinJ.29.1780   ATP-binding cassette protein subfamily H, member 2, putative
Leishmania infantum LinJ.11.0040   ATP-binding cassette protein subfamily H, member 1, putative
Leishmania major LmjF.11.0040   ATP-binding cassette protein subfamily H, member 1, putative
Leishmania major LmjF.29.1640   ATP-binding cassette protein subfamily H, member 2, putative
Leishmania mexicana LmxM.11.0040   ABC transporter, putative
Leishmania mexicana LmxM.08_29.1640   ABC transporter domain protein, putative
Mycobacterium tuberculosis Rv0986   Probable adhesion component transport ATP-binding protein ABC transporter
Mycobacterium tuberculosis Rv2564   Probable glutamine-transport ATP-binding protein ABC transporter GlnQ
Mycobacterium tuberculosis Rv0073   Probable glutamine-transport ATP-binding protein ABC transporter
Mycobacterium ulcerans MUL_0674   ABC transporter ATP-binding protein
Neospora caninum NCLIV_058820   ABC transporter, putative
Schmidtea mediterranea mk4.028825.00  
Trypanosoma cruzi TcCLB.509669.30   ABC transporter, putative
Trypanosoma cruzi TcCLB.510381.20   ABC transporter, putative
Trypanosoma cruzi TcCLB.506905.40   ABC transporter, putative
Trypanosoma cruzi TcCLB.509617.80   ABC transporter, putative
Treponema pallidum TP0581   ABC transporter, ATP-binding protein
Treponema pallidum TP0964   ABC transporter, ATP-binding protein
Wolbachia endosymbiont of Brugia malayi Wbm0023   peptide ABC transporter ATPase

Essentiality

Rv2564 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2608 this record Mycobacterium tuberculosis non-essential nmpdr
b0495 Escherichia coli non-essential goodall
b0879 Escherichia coli non-essential goodall
b1117 Escherichia coli essential goodall
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2564 (Mycobacterium tuberculosis), Probable glutamine-transport ATP-binding protein ABC transporter GlnQ
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