Detailed view for Rv1485

Basic information

TDR Targets ID: 6209
Mycobacterium tuberculosis, Ferrochelatase HemZ (protoheme ferro-lyase) (heme synthetase)

Source Database / ID:  Tuberculist 

pI: 5.9094 | Length (AA): 344 | MW (Da): 37144 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00762   Ferrochelatase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004325   ferrochelatase activity  
GO:0006783   heme biosynthetic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 295 2h1v (A) 4 304 24.00 0 1 1.19005 -0.57
3 321 3hcn (A) 68 411 24.00 0 1 1.20973 -0.44

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase. hasan
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127510)

Species Accession Gene Product
Arabidopsis thaliana AT2G30390   ferrochelatase 2
Arabidopsis thaliana AT5G26030   ferrochelatase 1
Brugia malayi Bm1_14320   ferrochelatase family protein
Candida albicans CaO19.1880   ferrochelatase
Candida albicans CaO19.9436   ferrochelatase
Chlamydia trachomatis CT_485   ferrochetalase
Dictyostelium discoideum DDB_G0288891   hypothetical protein
Drosophila melanogaster Dmel_CG2098   Ferrochelatase
Escherichia coli b0475   ferrochelatase
Echinococcus granulosus EgrG_000129100   ferrochelatase
Echinococcus multilocularis EmuJ_000129100   ferrochelatase
Homo sapiens 2235   ferrochelatase
Leishmania braziliensis LbrM.17.1610   ferrochelatase-like protein
Leishmania donovani LdBPK_171580.1   ferrochelatase-like protein
Leishmania infantum LinJ.17.1580   ferrochelatase-like protein
Leishmania major LmjF.17.1460   ferrochelatase-like protein
Leishmania mexicana LmxM.17.1460   ferrochelatase-like protein
Loa Loa (eye worm) LOAG_00220   hypothetical protein
Mycobacterium leprae ML1805c   FERROCHELATASE HEMZ (PROTOHEME FERRO-LYASE) (HEME SYNTHETASE)
Mus musculus ENSMUSG00000024588   ferrochelatase
Mycobacterium tuberculosis Rv1485   Ferrochelatase HemZ (protoheme ferro-lyase) (heme synthetase)
Mycobacterium ulcerans MUL_1493   ferrochelatase
Neospora caninum NCLIV_027860   Ferrochelatase (EC 4.99.1.1), related
Oryza sativa 4346651   Os09g0297000
Oryza sativa 4338537   Os05g0361200
Onchocerca volvulus OVOC11860   Ferrochelatase, mitochondrial homolog
Plasmodium berghei PBANKA_1140700   ferrochelatase
Plasmodium falciparum PF3D7_1364900   ferrochelatase
Plasmodium knowlesi PKNH_1106400   ferrochelatase, putative
Plasmodium vivax PVX_115205   ferrochelatase, putative
Plasmodium yoelii PY02777   Ferrochelatase
Saccharomyces cerevisiae YOR176W   ferrochelatase HEM15
Schistosoma japonicum Sjp_0114760   ko:K01772 ferrochelatase [EC4.99.1.1], putative
Schistosoma mansoni Smp_140790   ferrochelatase
Schmidtea mediterranea mk4.075368.01   Ferrochelatase, mitochondrial
Schmidtea mediterranea mk4.001050.02   Ferrochelatase, mitochondrial
Toxoplasma gondii TGME49_258650   protoheme ferro-lyase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0719   ferrochelatase

Essentiality

Rv1485 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1509 this record Mycobacterium tuberculosis essential nmpdr
b0475 Escherichia coli essential goodall
YOR176W Saccharomyces cerevisiae inviable yeastgenome
TGME49_258650 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) Mycobacterium tuberculosis No drug identifiers listed for this gene.
Annotator: crowther@u.washington.edu. Comment: 2007-12-07. References: 15850757

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ferrochelatase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

4 literature references were collected for this gene.

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Gene identifier Rv1485 (Mycobacterium tuberculosis), Ferrochelatase HemZ (protoheme ferro-lyase) (heme synthetase)
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