Detailed view for CaO19.922

Basic information

TDR Targets ID: 653903
Candida albicans, cytochrome P450 lanosterol 14-alpha -demethylase
Source Database / ID:  KEGG  

pI: 7.1325 | Length (AA): 528 | MW (Da): 60675 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00067   Cytochrome P450

Gene Ontology

Mouse over links to read term descriptions.
GO:0016705   oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen  
GO:0020037   heme binding  
GO:0005506   iron ion binding  
GO:0004497   monooxygenase activity  
GO:0055114   oxidation reduction  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 524 4lxj (A) 6 525 56.00 0 1 1.70273 -0.94
45 528 5tz1 (A) 45 528 99.00 0 1 2.17397 -1.77

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129288)

Species Accession Gene Product
Arabidopsis thaliana AT1G11680   sterol 14-demethylase
Candida albicans CaO19.8538   cytochrome P450 lanosterol 14-alpha -demethylase
Candida albicans CaO19.922   cytochrome P450 lanosterol 14-alpha -demethylase
Dictyostelium discoideum DDB_G0279403   cytochrome P450 family protein
Homo sapiens ENSG00000001630   cytochrome P450, family 51, subfamily A, polypeptide 1
Leishmania braziliensis LbrM.11.0880   lanosterol 14-alpha-demethylase, putative
Leishmania donovani LdBPK_111100.1   Lanosterol 14-alpha demethylase
Leishmania infantum LinJ.11.1100   lanosterol 14-alpha-demethylase, putative
Leishmania major LmjF.11.1100   lanosterol 14-alpha-demethylase, putative
Leishmania mexicana LmxM.11.1100   lanosterol 14-alpha-demethylase, putative
Mus musculus ENSMUSG00000001467   cytochrome P450, family 51
Mycobacterium tuberculosis Rv0764c   Cytochrome P450 51 Cyp51 (CYPL1) (P450-L1A1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) (P450-14DM)
Mycobacterium ulcerans MUL_0473   cytochrome P450 51B1 Cyp51B1
Oryza sativa 4350614   Os11g0525200
Oryza sativa 4338097   Os05g0211100
Saccharomyces cerevisiae YHR007C   sterol 14-demethylase
Trypanosoma brucei gambiense Tbg972.11.7000   lanosterol 14-alpha-demethylase,cytochrome P450 51A1, putative
Trypanosoma brucei Tb927.11.6210   Lanosterol 14-alpha demethylase
Trypanosoma congolense TcIL3000.11.6740   Lanosterol 14-alpha demethylase
Trypanosoma cruzi TcCLB.510101.50   Lanosterol 14-alpha demethylase
Trypanosoma cruzi TcCLB.506297.260   Lanosterol 14-alpha demethylase

Essentiality

CaO19.922 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu779 Mycobacterium tuberculosis non-essential nmpdr
Tb11.02.4080 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.4080 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.4080 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.4080 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YHR007C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSCA1100) (Aspergillus fumigatus) 14-alpha sterol demethylase Cyp51A Compounds References
Trypanosoma cruzi Lanosterol 14-alpha demethylase Compounds References
Neosartorya fumigata 14-alpha sterol demethylase Compounds References
Penicillium digitatum Cytochrome P450 14alpha-demethylase Compounds References
Trypanosoma cruzi Lanosterol 14-alpha demethylase Compounds References
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) Lanosterol 14-alpha demethylase Compounds References
Mycobacterium tuberculosis Cytochrome P450 51 Cyp51 (CYPL1) (P450-L1A1) (sterol 14-alpha demethylase) (lanosterol 14-alpha demethylase) (P450-14DM) Compounds References
Sorghum bicolor Obtusifoliol 14-alpha demethylase Compounds References
Leishmania major lanosterol 14-alpha-demethylase, putative Compounds References
Rattus norvegicus Cytochrome P450 51 Compounds References
Homo sapiens cytochrome P450, family 51, subfamily A, polypeptide 1 Compounds References
Penicillium digitatum P450 sterol 14-alpha-demethylase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cytochrome P450 7A1 503 aa 20.9% 521 aa Compounds References
Rattus norvegicus Cytochrome P450 1A2 513 aa 21.6% 524 aa Compounds References
Rattus norvegicus Cytochrome P450 2D3 500 aa 21.8% 477 aa Compounds References
Rattus norvegicus Cytochrome P450 1A1 524 aa 20.3% 488 aa Compounds References
Rattus norvegicus Cytochrome P450 2D18 500 aa 22.1% 466 aa Compounds References
Rattus norvegicus Cytochrome P450 11A1 526 aa 21.2% 462 aa Compounds References
Canis familiaris Cytochrome P450 2B11 494 aa 23.7% 451 aa Compounds References
Rattus norvegicus Cytochrome P450 2D1 504 aa 20.9% 478 aa Compounds References
Rattus norvegicus Cytochrome P450 2D2 500 aa 20.7% 473 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Anticandida activity- (MIC90 compound) / (MIC90 bifonazole) ChEMBL
  • Anticandida activity- (MIC90 compound) / (MIC90 bifonazole)
  • Inhibition of Candida albicans SC5314 CYP51 assessed as eburicol composition of total sterols at 8 ug/ml by GC-MS method relative to control LITERATURE
  • Inhibition of Candida albicans SC5314 CYP51 assessed as eburicol composition of total sterols at 8 ug/ml by GC-MS method relative to control.
  • Inhibition of Candida albicans SC5314 CYP51 assessed as change in total sterol composition at 8 ug/ml by GC-MS method (Rvb = 7 LITERATURE
  • Inhibition of Candida albicans SC5314 CYP51 assessed as change in total sterol composition at 8 ug/ml by GC-MS method (Rvb = 7. 1 %)
  • Inhibition of Candida albicans SC5314 CYP51 assessed as ergosterol composition of total sterols at 8 ug/ml by GC-MS method (Rvb = 89 LITERATURE
  • Inhibition of Candida albicans SC5314 CYP51 assessed as ergosterol composition of total sterols at 8 ug/ml by GC-MS method (Rvb = 89. 4 %)
  • Inhibition of Candida albicans SC5314 CYP51 assessed as lanosterol composition of total sterols at 8 ug/ml by GC-MS method (Rvb = 3 LITERATURE
  • Inhibition of Candida albicans SC5314 CYP51 assessed as lanosterol composition of total sterols at 8 ug/ml by GC-MS method (Rvb = 3. 5 %)
  • Inhibition of Candida albicans SC5314 CYP51 assessed as obtusifoliol composition of total sterols at 8 ug/ml by GC-MS method relative to control LITERATURE
  • Inhibition of Candida albicans SC5314 CYP51 assessed as obtusifoliol composition of total sterols at 8 ug/ml by GC-MS method relative to control.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier CaO19.922 (Candida albicans), cytochrome P450 lanosterol 14-alpha -demethylase
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