Detailed view for Rv2156c

Basic information

TDR Targets ID: 6555
Mycobacterium tuberculosis, Probable phospho-N-acetylmuramoyl-pentappeptidetransferase MurX
Source Database / ID:  Tuberculist 

pI: 8.9159 | Length (AA): 359 | MW (Da): 37713 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 10

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00953   Glycosyl transferase family 4
PF10555   Phospho-N-acetylmuramoyl-pentapeptide-transferase signature 1

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0016021   integral to membrane  
GO:0008963   phospho-N-acetylmuramoyl-pentapeptide-transferase activity  
GO:0009252   peptidoglycan biosynthetic process  

Metabolic Pathways

Peptidoglycan biosynthesis (KEGG)
Global map (KEGG)
Vancomycin resistance (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 345 5jnq (A) 2 323 38.00 0 0.96 1.052 0.47
3 350 5ckr (A) 25 357 32.00 0 1 1.29176 -0.13
5 349 5ckr (A) 27 356 30.00 0 0.96 1.2598 0.02

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_131597)

Species Accession Gene Product
Arabidopsis thaliana AT4G18270   phospho-N-acetylmuramoyl-pentapeptide-transferase-like protein
Chlamydia trachomatis CT_757   phospho-N-acetylmuramoyl-pentapeptide-transferase
Escherichia coli b0087   phospho-N-acetylmuramoyl-pentapeptide transferase
Mycobacterium leprae ML0911   Probable phospho-N-acetylmuramoyl-pentappeptidetransferase MurX
Mycobacterium tuberculosis Rv2156c   Probable phospho-N-acetylmuramoyl-pentappeptidetransferase MurX
Mycobacterium ulcerans MUL_3504   phospho-N-acetylmuramoyl-pentapeptide-transferase
Oryza sativa 4325561   Os01g0210800
Treponema pallidum TP0345   phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY)
Wolbachia endosymbiont of Brugia malayi Wbm0643   phospho-N-acetylmuramoyl-pentapeptide-transferase

Essentiality

Rv2156c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2189 this record Mycobacterium tuberculosis essential nmpdr
b0087 Escherichia coli essential goodall
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

Compound Source Reference
ChEMBL23 References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Staphylococcus aureus Phospho-N-acetylmuramoyl-pentapeptide-transferase Compounds References
Bacillus subtilis (strain 168) Phospho-N-acetylmuramoyl-pentapeptide-transferase Compounds References
Escherichia coli phospho-N-acetylmuramoyl-pentapeptide transferase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.3259 1 0.5
0.3418 0.2923 1
1.5597 1 1
0.513 0.2755 1
2.6373 0.3346 1
0.1018 0.4305 1
4.5544 1 0.5
0.0896 0.5319 1
0.0744 0.9272 1
3.1566 1 1
0.1258 1 1
0.4895 0.731 1
0.666 1 1
0.9873 1 1
2.9136 1 1
0.1581 1 0.5
0.2321 1 0.5
2.7732 0.4105 1
0.231 1 0.5
3.0394 1 1
0.0729 0.2649 0.2621
3.6456 1 1
4.9104 1 1
0.0896 1 1
0.5101 1 0.5
0.1658 0.2815 1
0.0638 1 1
0.0802 0.3406 1
3.6077 1 1
4.9298 1 0.5
0.3073 1 1
0.0896 0.3763 1
0.1503 1 0.5
0.0896 0.6367 1
0.6547 1 1
0.2646 0.2771 1
3.6077 1 1
2.5633 1 1
2.2001 0.9409 1
0.147 1 1
0.0896 1 0.5
1.8755 0.3592 1
2.4403 1 1
1.7179 1 1
0.0722 0.2649 0.2621
0.0228 0.5749 0.5
2.7464 1 1
2.1295 1 1
2.5747 0.9832 1
3.4178 1 1
0.0896 1 0.5
0.0772 0.4761 1
0.3079 1 0.5
2.9912 1 1
3.6077 1 1
0.1733 0.3621 1
0.0511 1 0.5
3.6136 1 0.5
3.6077 1 1
0.232 1 0.5
0.0896 0.3763 1
3.6077 1 1
1.9069 0.8961 1
1.5668 0.7216 1
0.0896 1 1
2.7114 1 1
0.0896 1 0.5
2.9136 1 1
0.1687 0.6922 1
0.2253 1 1
3.6077 1 1
0.0884 1 0.5
0.0511 1 1
0.6657 1 0.5
1.7117 0.3023 1
2.1295 1 1
0.0352 1 0.5
3.3221 1 1
0.0722 0.2649 0.2635
0.0863 0.4937 0.5
0.144 0.5418 0.5401
2.0908 0.8566 1
0.0896 1 1
0.0774 0.9758 1
1.7179 0.8768 1
3.607 1 0.5
2.2595 0.8489 1
1.8755 0.3362 1
0.144 0.5418 0.5401
0.2727 1 1
0.3262 1 1
0.8178 1 0.5
1.99009 1 1
4.9254 1 0.5
2.975 1 0.5
3.6077 1 1
0.072 0.2621 0.2621
0.0552 0.5395 0.5392
0.0511 0.4305 0.5
0.0771 0.5456 1
0.2965 1 1
0.224 1 1
3.8606 1 1
3.2043 1 1
4.9254 0.4273 1
4.4231 1 1
0.666 1 1
2.3095 0.8631 1
0.5666 0.3082 1
0.5671 1 1
0.0896 0.4774 1
0.1397 0.49 0.4899
0.3121 1 1
3.6077 1 1
2.3095 0.8861 1
0.0638 1 1
0.0758 1 1
2.7114 1 1
1.7179 0.8768 1
0.4173 0.702 1
0.1508 1 0.5
1.9069 0.8961 1
0.0802 0.3406 1
2.6966 0.8555 1
2.1608 0.2973 1
0.0896 1 1
0.9671 1 0.5
0.2727 1 0.5
0.1527 1 1
2.7732 0.4105 1
0.5921 0.3715 1
3.6077 1 1
2.9225 1 1
3.6077 1 1
0.0896 1 0.5
0.0179 0.3151 1
0.0896 0.98 0.5
0.2198 0.8422 1
0.0552 0.5398 0.5392
3.6077 1 1
0.3452 0.3443 1
0.3452 0.301 1
0.3262 1 1
1.6529 0.2776 1
0.013 0.3138 1
2.7464 1 1
2.7732 0.4105 1
0.0896 1 1
0.1835 1 0.5
0.0896 0.9033 1
4.6991 1 1
1.7117 0.31 1
1.6146 0.5034 1
0.666 1 1
0.8749 1 0.5
0.0511 0.4305 0.5
1.7117 0.3023 1
1.3557 0.3357 1
0.0732 1 1
0.1658 0.2815 1
0.1735 1 1
0.4534 0.6934 1
0.072 0.5052 1
0.0896 1 0.5
3.6077 1 1
4.3688 1 1
0.0435 1 1
0.7609 1 0.5
0.0722 0.2649 0.2635
1.8755 0.3053 1
2.9912 1 1
2.5959 1 1
0.1349 1 0.5
0.0816 1 1
2.7732 0.4105 1
0.0722 0.2649 0.2635
3.6077 1 1
0.1527 1 0.5
0.0289 0.3825 0.3825
1.8755 0.3592 1
2.9225 1 1
0.149 1 0.5
0.666 1 1
0.3103 1 1
0.1676 1 1
0.0721 0.265 0.2621
0.5238 0.4955 1
0.0764 0.5365 1
1.7179 0.8768 1
0.0511 0.3591 1
3.6077 1 1
0.0378 0.4695 0.7366
0.0896 0.2572 1
0.0771 0.5456 1
3.6077 1 1
0.0765 1 1
0.6547 1 1
1.2957 0.3403 0.625
0.5944 0.2621 0.2621
2.9374 1 1
0.1349 1 0.5
3.6313 1 0.5
0.0766 0.5562 1
0.0896 0.6244 1
0.0868 1 1
0.0759 0.547 1
0.8033 1 0.5
0.0335 0.362 1
1.0472 1 0.5
0.0867 1 1
4.8818 1 1
0.1736 1 0.5
0.666 1 1
0.0896 0.5319 1
2.0187 0.8131 1
1.5597 1 1
3.3548 0.9467 0.9467
0.3121 1 1
0.0896 0.3763 1
0.1585 0.383 0.383
0.0896 0.2572 1
0.0778 1 1
2.9136 1 1
0.637389 0.980134 1
3.3221 1 1
0.0883 1 0.5
0.0719 0.2641 0.2621
0.0289 0.3825 0.3825
3.6077 1 1
2.3369 1 1
0.1035 0.4128 1
0.1527 1 1
0.0378 0.4695 0.7366
3.6077 1 1
3.2086 1 0.5
3.4849 1 1
0.0896 1 1
2.0982 1 1
0.0896 0.3763 1
0.0896 0.6375 1
0.0898 0.3504 1
0.5944 0.2621 0.2621
3.3548 1 1
0.5943 0.2634 1
0.0767 0.9592 1
0.0834 0.2941 1
0.0896 1 0.5
0.51 1 1
0.2333 1 0.5
0.0718 0.2655 0.2621
0.0896 0.9048 0.5
0.0795 1 0.5
3.6077 1 1
0.1832 0.5 0.5
1.4854 0.282 1
0.0758 1 1
0.0896 0.3763 1
2.5589 1 1
0.0896 0.4557 0.5
0.2848 1 1
0.0747 1 0.5
3.6077 1 1
4.3954 1 1
3.6313 1 0.5
4.9778 1 0.5
0.5943 0.2634 1
1.899 1 0.5
0.0896 1 0.5
1.8755 0.3362 1
3.2086 1 0.5
0.2504 1 0.5
0.0896 0.2572 1
3.6077 1 1
4.6815 1 0.5
0.5454 0.7019 1
0.5101 1 0.5
0.0868 1 1
2.2821 0.7545 1
2.554 1 1
0.666 1 1
1.0472 1 0.5
0.0511 0.3591 1
2.1295 0.3166 1
0.0802 0.3406 1
1.8755 0.4772 1
0.0896 1 0.5
1.6146 0.4901 1
1.8076 0.8489 1
0.1471 1 0.5
3.8606 1 1
0.0721 1 1
4.4924 1 1
0.2551 0.2898 1
0.0964 1 1
0.1658 0.2815 1
0.1744 1 1
1.0472 1 0.5
3.9621 1 0.5
0.0137 0.296 0.4046
1.6335 1 0.5
0.0896 0.4322 0.5
0.0896 1 1
0.4475 0.5877 1
0.0896 1 0.5
0.1736 1 1
2.4147 0.3346 1
1.4854 0.282 1
3.6077 1 1
0.0758 0.5374 1
3.8606 1 1
2.535 1 1
2.1295 0.3245 1
1.2012 1 0.5
0.2253 1 1
0.019 0.4828 0.6516
0.1835 1 0.5
0.0743 1 0.5
0.0896 0.6367 1
4.1639 1 0.5
0.0896 0.669 1
0.2583 1 0.5
1.7117 0.31 1
1.3557 0.3357 1
1.3239 0.3354 0.549
2.5589 1 1
2.0966 1 0.5
2.1276 1 0.5
2.1295 1 1
2.89408 1 1
1.8755 0.3174 1
2.4147 0.3346 1
2.0127 1 1
0.0889 0.9422 1
2.9136 1 1
1.9697 0.3022 1
0.0739 0.4478 1
0.1722 1 0.5
4.8535 1 1
3.6077 1 1
0.0378 0.4646 0.7289
0.1349 1 0.5
0.0795 1 0.5
0.0896 0.5863 0.5
3.2043 1 1
1.7179 1 1
0.0802 0.3406 1
0.1758 1 0.5
1.8755 0.3362 1
0.1651 1 0.5
2.4706 0.8555 1
0.0355 0.5216 1
0.1799 1 1
0.0911 0.3936 0.3934
0.0833 1 1
0.3951 0.3216 1
3.4849 1 1
0.1497 1 0.5
0.1715 1 1
0.0737 0.6257 1
3.7669 1 0.5
0.3044 1 1
3.6077 1 1
0.0896 1 1
0.0896 1 1
3.6077 1 1
0.9671 1 0.5
2.4147 0.3346 1
0.0896 1 0.5
0.0811 1 0.5
0.0682 1 1
0.0724 0.2649 0.2621
0.5944 0.2621 0.2621
2.7864 1 1
0.5921 0.3715 1
2.0982 1 1
0.0896 1 1
0.0779383 1 0.5
3.6313 1 0.5
1.8755 0.4772 1
4.7657 1 0.5
0.0442 0.4227 0.4213
0.019 0.4828 0.6516
2.9374 1 1
0.1583 1 0.5
1.4854 0.282 1
3.6077 1 1
2.7114 1 1
0.2228 1 1
0.0882 1 0.5
2.2595 0.8489 1
0.1349 1 0.5
2.4706 0.8555 1
6.9308 1 1
3.6265 1 0.5
1.8076 0.8489 1
2.5117 1 0.5
3.674 1 0.5
0.1708 1 1
0.0863 0.4937 0.5
0.0896 1 0.5
1.0454 1 1
2.0354 0.2553 0.6963
3.6077 1 1
2.535 1 1
3.8018 1 0.5
0.0896 0.6367 1
0.0896 1 1
0.013 0.3138 1
0.0647 0.6567 1
0.0896 0.2572 1
0.5053 1 1
0.1481 1 0.5
0.3349 0.4411 1
3.6077 1 1
0.0889 0.9422 1
2.2305 1 1
0.1479 1 0.5
0.2727 1 0.5
0.0511 0.3591 1
0.5888 1 1
3.0394 1 1
0.0877 0.3338 1
2.4706 1 1
0.0896 0.5319 1
0.0896 0.5331 1
4.3688 1 1
1.0472 1 0.5
0.5921 0.3715 1
0.5256 1 1
2.3741 1 1
3.4737 1 1
1.8755 0.3053 0.5669
3.7515 1 0.5
0.0842 1 1
2.3142 1 1
4.7657 1 0.5
0.2646 1 1
0.0802 1 1
0.2321 1 0.5
3.3548 1 1
0.7299 1 0.5
0.0896 0.5924 1
2.2595 0.8489 1
0.0896 0.2572 1
3.6077 1 1
3.6077 1 1
0.075 0.5396 1
0.231 0.6365 1
0.1489 1 0.5
3.8606 1 1
0.666 1 1
3.8606 1 1
0.1427 0.7598 1
0.0718 0.4312 1
0.1835 1 0.5
4.6273 1 0.5
0.0285 0.3491 0
0.5944 0.2621 0.2621
1.5068 1 1
0.0896 0.3763 1
0.5063 1 0.5
0.0722 0.2649 0.2635
2.9374 1 1
3.8606 1 1
0.0896 1 1
4.9463 1 0.5
0.8895 1 1
0.0896 1 1
4.4924 1 1
0.1789 1 1
0.0896 0.9593 0.5
0.072 0.2702 0.2698
0.1658 0.2815 1
0.2727 1 1
0.0738 0.5045 1
4.6991 1 1
2.6373 0.3433 1
3.3221 1 1
1.0454 1 1
0.0834 0.2941 1
1.2957 0.3403 0.625
0.0896 1 1
0.2605 1 0.5
1.5668 0.7216 1
3.6077 1 1
2.4147 0.3346 1
0.2264 1 1
2.3142 1 1
0.019 0.4828 0.6516
0.0964 1 1
2.5959 1 1
1.7179 0.8768 1
4.8818 1 1
1.9697 0.3021 1
0.0834 0.2941 1
1.8755 0.3362 1
0.0341 0.3517 1
1.3606 0.3931 1
0.6207 1 1
0.0896 0.3969 1
0.1578 1 0.5
0.1787 1 0.5
0.1505 1 1
4.4231 1 1
3.6077 1 1
3.6077 1 1
0.1465 1 0.5
2.7732 0.4105 1
0.0896 1 1
3.3548 1 1
3.8606 1 1
0.0488 0.6597 0.5
0.072 0.5052 1
3.6077 1 1
0.1688 1 0.5
0.1452 1 0.5
0.1578 1 0.5
2.4147 0.3346 1
2.0982 1 1
2.1295 1 1
1.9697 0.3022 1
0.2198 0.8422 1
0.0717 0.4395 1
0.3103 1 1
0.1503 1 0.5
1.8755 0.4772 1
3.8606 1 1
0.0511 0.4305 0.5
0.1254 1 1
2.5633 1 1
0.0802 0.3406 1
0.0896 1 1
0.2329 1 1
0.1841 1 0.5
2.4403 1 1
2.554 1 1
0.1733 1 1
0.2223 1 0.5
0.1578 1 0.5
0.2345 1 0.5
0.0867 1 1
2.6748 1 0.5
0.2223 1 0.5
0.5758 0.4894 1
2.3846 1 1
4.9778 1 0.5
1.8755 0.4772 1
1.3488 0.547 0.547
0.0771 0.5456 1
4.5445 1 0.5
3.8606 1 1
0.0896 1 1
0.0776 0.9299 1
0.5758 0.4894 1
1.0472 1 0.5
4.9254 0.4273 1
0.0719 0.2641 0.2621
2.0908 0.8792 1
3.8606 1 1
2.7342 1 1
0.0943 0.449 1
0.6278 1 1
1.3557 0.3357 1
0.0896 0.9967 1
2.1608 0.2973 1
0.1152 0.4521 1
4.0321 1 0.5
2.0908 0.8792 1
0.0896 0.5319 1
3.3548 1 1
2.0127 1 1
0.231 0.6365 1
3.1842 1 1
0.0736 0.4968 1
2.3846 1 1
0.0896 0.2572 1
2.5747 0.9832 1
0.666 1 1
4.9254 0.4273 1
0.2551 0.2898 1
0.1658 0.2815 1
0.6657 1 0.5
0.0808 1 0.5
0.0875 1 0.5
4.9254 0.4273 1
0.2234 1 1
0.0896 0.5319 1
3.4737 1 1
2.5747 0.9832 1
0.2583 1 1
2.9136 1 1
0.1397 1 1
0.0896 0.5331 1
0.5101 1 0.5
0.0896 1 1
2.9136 1 1
2.2305 1 1
0.166 1 0.5
0.0725 0.2649 0.2621
0.0736 0.9893 1
0.9447 1 0.5
0.0896 1 0.5
0.0896 1 1
3.6077 1 1
1.8755 0.4772 1
0.0802 0.3406 1
0.333308 0.342452 1
3.6077 1 1
1.0946 1 1
0.1152 0.4521 1
0.1035 0.4128 1
2.9136 1 1
0.0802 0.3406 1
0.666 1 1
0.0896 0.3446 1
0.0752 0.9152 1
0.3762 0.3037 1
2.9912 1 1
1.8755 0.3083 0.567
0.0722 0.2649 0.2635
4.3688 1 1
0.0896 1 1
0.4895 0.731 1
3.6077 1 1
2.7114 1 1

Assayability

Assay information

  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis MraY translocase

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2156c (Mycobacterium tuberculosis), Probable phospho-N-acetylmuramoyl-pentappeptidetransferase MurX
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