Detailed view for Rv1316c

Basic information

TDR Targets ID: 6622
Mycobacterium tuberculosis, Methylated-DNA--protein-cysteine methyltransferase Ogt (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltra

Source Database / ID:  Tuberculist 

pI: 6.3298 | Length (AA): 165 | MW (Da): 17858 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01035   6-O-methylguanine DNA methyltransferase, DNA binding domain
PF02870   6-O-methylguanine DNA methyltransferase, ribonuclease-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003908   methylated-DNA-[protein]-cysteine S-methyltransferase activity  
GO:0003824   catalytic activity  
GO:0006281   DNA repair  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 165 4bhb (A) 1 165 99.00 0 1 2.1966 -1.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

  • 4BHB:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 4BHC:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. hasan
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127355)

Species Accession Gene Product
Candida albicans CaO19.211   similar to bacterial Ada regulatory protein
Candida albicans CaO19.3108   6-O-methylguanine-DNA methylase
Candida albicans CaO19.7843   similar to bacterial Ada regulatory protein
Candida albicans CaO19.10620   6-O-methylguanine-DNA methylase
Caenorhabditis elegans CELE_Y62E10A.5   Protein AGT-1, isoform B
Chlamydia trachomatis CT_477   methylated-DNA protein-cysteine methyltransferase
Dictyostelium discoideum DDB_G0275095   6-O-methylguanine-DNA methyltransferase
Drosophila melanogaster Dmel_CG1303   O-6-alkylguanine-DNA alkyltransferase
Escherichia coli b1335   O-6-alkylguanine-DNA:cysteine-protein methyltransferase
Escherichia coli b2213   fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase
Homo sapiens 4255   O-6-methylguanine-DNA methyltransferase
Leishmania braziliensis LbrM.26.1730   hypothetical protein, conserved
Leishmania donovani LdBPK_261800.1   methylated-DNA-[protein]-cysteine S-methyltransferase, putative
Leishmania infantum LinJ.26.1800   hypothetical protein, conserved
Leishmania major LmjF.26.1800   hypothetical protein, conserved
Leishmania mexicana LmxM.26.1800   hypothetical protein, conserved
Mycobacterium leprae ML1151c   PROBABLE METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE OGT (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA
Mus musculus ENSMUSG00000054612   O-6-methylguanine-DNA methyltransferase
Mycobacterium tuberculosis Rv1316c   Methylated-DNA--protein-cysteine methyltransferase Ogt (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltra
Mycobacterium ulcerans MUL_3946   methylated-DNA--protein-cysteine methyltransferase Ogt
Saccharomyces cerevisiae YDL200C   Mgt1p
Trypanosoma cruzi TcCLB.508347.20   O-6 methyl-guanine alkyl transferase, putative
Trypanosoma cruzi TcCLB.506145.30   O-6 methyl-guanine alkyl transferase, putative
Treponema pallidum TP0141   methylated-DNA-protein-cysteine S-methyltransferase (dat)
Trichomonas vaginalis TVAG_401340   methylated-DNA--protein-cysteine methyltransferase, putative

Essentiality

Rv1316c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1338 this record Mycobacterium tuberculosis non-essential nmpdr
b2213 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens O-6-methylguanine-DNA methyltransferase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0767 0.6224 1
0.0638 0.7501 1
0.0804 0.3357 1
0.0805 0.8215 1
0.0124 0.3338 0.9275
0.0847 0.891 1
0.1222 1 0.5
0.0416 1 0.5
0.0407 0.4545 1
0.0876 0.7698 1
0.0407 0.4545 1
0.0247 0.2553 0.5148
0.0399 0.4027 1
0.0834 1 0.5
0.1219 1 0.5
0.0642 0.6608 1
0.0781 0.7111 1
0.1558 0.4109 1
0.079 0.9795 1
0.1051 1 0.5
0.1074 1 0.5
0.1032 1 0.5
0.0616 1 0.5
0.0233 0.3085 0.2779
0.0233 0.4281 0.5
0.0804 0.3357 1
0.0233 0.4655 0
0.0233 0.4625 0.5
0.063 0.6614 1
0.0851 0.3466 1
0.0796 1 0.5
0.0392 0.5429 0.5
0.0648 1 1
0.0233 0.3277 0.1846
0.0781 0.7111 1
0.0601 0.3894 0.9385
0.0386 0.261 1
0.0386 0.261 1
0.0233 0.6233 1
0.0233 0.318 0.5
0.0391 1 0.5
0.0608 0.6634 1
0.0781 0.7111 1
0.0393 0.4551 1
0.0642 0.6608 1
0.044 0.333 0.5
0.1048 1 1
0.0405 1 0.5
0.0124 0.3371 0.9275
0.0619 0.6626 1
0.107941 1 1
0.0648 1 1
0.0836 0.9053 1
0.0619 0.6626 1
0.0608 1 0.5
0.0584 1 0.5
0.0608 0.6634 1
0.0577 1 0.5
0.1197 1 0.5
0.0416 1 1
0.1009 0.6966 1
0.1628 1 0.5
0.0386 0.261 1
0.0813 0.9202 0.5
0.0233 0.2678 1
0.0233 0.6545 1
0.0233 0.8063 0.5
0.0385 0.4634 0.5575
0.1021 1 0.5
0.038 0.4682 1
0.0233 0.3279 1
0.1002 0.6129 1
0.0233 1 0.5
0.0233 1 0.5
0.1208 1 0.5
0.0781 0.7111 1
0.0608 0.3639 1
0.0787 1 0.5
0.0838 1 0.5
0.0767 0.6224 1
0.0991 1 0.5
0.0838 1 0.5
0.0851 0.3466 1
0.2045 1 1
0.0597 0.6642 1
0.0975 0.9003 1
0.0233 0.424 1
0.0188 1 0.5
0.0233 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Rv1316c (Mycobacterium tuberculosis), Methylated-DNA--protein-cysteine methyltransferase Ogt (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltra
Title for this comment
Comment