Detailed view for Rv2351c

Basic information

TDR Targets ID: 6821
Mycobacterium tuberculosis, Membrane-associated phospholipase C 1 PlcA (MTP40 antigen)

Source Database / ID:  Tuberculist 

pI: 6.4215 | Length (AA): 512 | MW (Da): 55381 | Paralog Number: 3

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04185   Phosphoesterase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016788   hydrolase activity, acting on ester bonds  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
25 463 2d1g (A) 12 488 20.00 0 0.47 0.882622 -0.25
41 422 2d1g (A) 32 449 25.00 0.000000049 0.99 0.784794 0.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_131170)

Species Accession Gene Product
Arabidopsis thaliana AT3G03530   non-specific phospholipase C4
Arabidopsis thaliana AT1G07230   non-specific phospholipase C1
Arabidopsis thaliana AT2G26870   non-specific phospholipase C2
Arabidopsis thaliana AT3G03540   non-specific phospholipase C5
Arabidopsis thaliana AT3G48610   non-specific phospholipase C6
Arabidopsis thaliana AT3G03520   non-specific phospholipase C3
Mycobacterium tuberculosis Rv2351c   Membrane-associated phospholipase C 1 PlcA (MTP40 antigen)
Mycobacterium tuberculosis Rv2350c   Membrane-associated phospholipase C 2 PlcB
Mycobacterium tuberculosis Rv2349c   Probable phospholipase C 3 PlcC
Mycobacterium tuberculosis Rv1755c   PROBABLE PHOSPHOLIPASE C 4 (FRAGMENT) PLCD
Mycobacterium ulcerans MUL_2799   membrane-associated phospholipase C2 PlcB
Oryza sativa 4325407   Os01g0955000
Oryza sativa 4326465   Os01g0102000
Oryza sativa 4350831   Os11g0593000
Oryza sativa 4334815   Os03g0852800
Oryza sativa 4334641   Os03g0826600

Essentiality

Rv2351c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1785 Mycobacterium tuberculosis non-essential nmpdr
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Phospholipase C (3.1.4.3 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Rv2351c cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Mycobacterium tuberculosis ( 1 )

Bibliographic References

35 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Rv2351c (Mycobacterium tuberculosis), Membrane-associated phospholipase C 1 PlcA (MTP40 antigen)
Title for this comment
Comment