Detailed view for EHI_177540

Basic information

TDR Targets ID: 797845
Entamoeba histolytica, thymidine kinase, putative

Source Database / ID:  AmoebaDB  

pI: 7.0256 | Length (AA): 218 | MW (Da): 24251 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00265   Thymidine kinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004797   thymidine kinase activity  
GO:0005524   ATP binding  

Metabolic Pathways

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
23 198 1w4r (A) 18 191 56.00 0 1 1.51554 -0.83
24 201 5fuw (A) 205 383 42.00 0 1 1.28671 -0.65
24 196 2j87 (A) 4 174 58.00 0 1 1.38228 -0.15
24 193 2b8t (A) 12 192 41.00 0 1 1.14152 0.02

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_129576)

Species Accession Gene Product
Arabidopsis thaliana AT5G23070   Thymidine kinase
Arabidopsis thaliana AT3G07800   thymidine kinase
Caenorhabditis elegans CELE_Y43C5A.5   Protein THK-1, isoform B
Dictyostelium discoideum DDB_G0289179   calmodulin-binding protein
Entamoeba histolytica EHI_177540   thymidine kinase, putative
Giardia lamblia GL50803_8364   Thymidine kinase
Homo sapiens ENSG00000167900   thymidine kinase 1, soluble
Leishmania braziliensis LbrM.21.1410   thymidine kinase, putative
Leishmania donovani LdBPK_211450.1   thymidine kinase, putative
Leishmania infantum LinJ.21.1450   thymidine kinase, putative
Leishmania major LmjF.21.1210   thymidine kinase, putative
Leishmania mexicana LmxM.21.1210   thymidine kinase, putative
Mus musculus ENSMUSG00000025574   thymidine kinase 1
Oryza sativa 4331376   Os03g0113100
Trypanosoma brucei gambiense Tbg972.10.960   thymidine kinase, putative
Trypanosoma brucei Tb927.10.880   thymidine kinase
Trypanosoma congolense TcIL3000_0_05400   thymidine kinase, putative
Trypanosoma congolense TcIL3000_0_01250   thymidine kinase, putative
Trypanosoma congolense TcIL3000_10_740   thymidine kinase, putative
Trypanosoma cruzi TcCLB.506855.260   thymidine kinase, putative
Trypanosoma cruzi TcCLB.511133.20   thymidine kinase, putative
Trichomonas vaginalis TVAG_050740   thymidine kinase, putative
Trichomonas vaginalis TVAG_083490   thymidine kinase, putative
Trichomonas vaginalis TVAG_344830   thymidine kinase, putative

Essentiality

EHI_177540 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.880 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.880 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.880 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.880 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y43C5A.5 Caenorhabditis elegans embryonic lethal wormbase
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Thymidine kinase Compounds References
Homo sapiens thymidine kinase 1, soluble Compounds References
Cricetulus griseus Thymidine kinase, cytosolic Compounds References
Ureaplasma parvum serovar 3 (strain ATCC 700970) Thymidine kinase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0492 0.3234 1
0.0251 0.6472 1
0.0511 0.3899 1
0.0301 0.3229 1
0.0484 0.5562 1
0.058 0.3885 1
0.0282 0.253 0.828
0.0245 0.6454 1
0.0275 0.5613 0.5
0.0504 0.3592 1
0.0277 0.592 1
0.0286 0.5816 0.5
0.0301 0.3552 1
0.3783 0.3186 1
0.0801 0.4786 1
0.0263 0.4241 1
0.0205 0.3235 1
0.0585 0.3897 1
0.0241 0.5554 1
0.0756 0.4351 1
0.0235 0.3535 1
0.0242 0.2505 1
0.1018 0.4725 1
0.1279 0.7589 1
0.0548 0.3346 0.8133
0.0251 0.3675 1
0.1031 0.6469 1
0.0499 0.6223 1
0.0777 0.4591 1
0.0246 0.6454 1
0.0247 0.2845 1
0.058 0.3903 1
0.0746 0.3034 1
0.0116717 1 1
0.0282 0.253 0.828
0.0252 0.4334 0.5
0.1507 0.9048 1
0.0521 0.3897 1
0.2342 0.2778 1
0.0266 0.3679 1
0.0277 0.4264 1
0.076 0.7988 1
0.0244 0.4581 1
0.0245 0.4755 1
0.0549 0.6713 1
0.0252 0.2949 1
0.1046 0.6399 1
0.0509 0.3903 1
0.0508 0.3277 1
0.051 0.3127 0.7497
0.0504 0.3897 1
0.0504 0.3592 1
0.0521 0.3051 1
0.0265 0.4803 1
0.0522 0.3174 1
0.0764 0.3165 1
0.201 0.4435 1
0.1044 0.4101 1
0.079 0.4741 1
0.0488 0.5756 0.5
0.0499 0.7566 1
0.0532 0.3107 1
0.078 0.4628 1
0.0274 0.3897 1
0.0244 0.3625 1
0.0511 0.313 1
0.0989 0.6718 1
0.0285 0.3565 1
0.0565 0.3165 1
0.0244 0.4886 1
0.2342 0.2778 1
0.0536 0.3897 1
0.0499 0.7566 1
0.0515 0.3087 1
0.052 0.4939 1
0.0248 0.2809 0
0.0753 0.4629 1
0.0254 0.4394 1
0.0489 0.3897 1
0.0495 0.3903 1
0.0498 0.9855 1
0.051 0.3823 1
0.0259 0.537 1
0.201 0.4435 1
0.0537 0.542 1
0.0273 0.33 1
0.0498 0.2759 1
0.0246 0.6454 1
0.0555 0.3897 1
0.101 0.6385 1
0.0745 0.5809 1
0.0249 0.2532 1
0.0523 0.308 1
0.2586 0.531 1
0.1044 0.8931 1
0.0256 0.2557 1
0.025 0.5279 0.5
0.0499 0.6205 1
0.1044 0.4101 1
0.0572 0.304 1
0.0278 0.2792 1
0.0548 0.3346 0.8133
0.0784 0.4703 1
0.025 0.272 1
0.0514 0.3156 1
0.0499 0.7566 1
0.0301 0.6876 0.5
0.0766 0.4548 1
0.0521 0.2626 1
0.1769 0.9697 1
0.1064 0.8933 1
0.0548 0.3346 0.8133
0.0756 0.4402 1
0.0247 0.2845 1
0.0286 0.4669 1
0.0765 0.3816 1
0.0504 0.3592 1
0.0494 0.549 1
0.0252 0.6429 1
0.0756 0.4351 1
0.1028 0.6282 1
0.228 0.8644 1
0.0518 0.3885 1
0.1852 0.9879 1
0.025 0.2576 1
0.0772 0.4698 1
0.0285 0.3565 1
0.0737 0.5852 1
0.0492 0.3234 1
0.1045 0.8442 0.5
0.3225 0.3926 1
0.0803 0.3778 1
0.0243 0.5395 1
0.1007 0.4752 1
0.0487 0.8859 1
0.1019 0.4044 0.649
0.056 0.3897 1
0.0268 0.3657 1
0.0751 0.4353 1
0.0276 0.3737 1
0.259 0.3097 1
0.0278 0.2792 1
0.0585 0.3883 1
0.0273 0.33 1
0.0246 0.5649 1
0.025 0.4154 1
0.0511 0.3877 1
0.3297 0.2635 0.5058
0.0528 0.6618 1
0.101 0.6388 1
0.0815 0.4977 1
0.0519 0.3044 1
0.0301 0.3229 1
0.0532 0.3891 1
0.0547 0.3897 1
0.0497 0.3903 1
0.0266 0.4022 1
0.0241 0.3399 1
0.1284 0.7947 1
0.0511 0.9224 1
0.259 0.3097 1
0.1047 0.4387 1
0.0494 0.5453 1
0.0242 0.6094 0.5
0.0494 0.5493 1
0.0251 0.7421 1
0.0273 0.33 1
0.026 0.395 0.5
0.0495 0.4062 1
0.0763 0.3025 1
0.0235 0.3518 1
0.0265 0.2702 1
0.0245 0.2884 1
0.0292 0.5363 1
0.0739 0.4023 1
0.0243 0.5388 1
0.0564 0.6717 1
0.0993 0.8072 1
0.0511 0.9224 1
0.0247 0.4527 0.9981
0.2342 0.2778 1
0.0254 0.2863 0.5
0.0284 0.4965 1
0.0241 0.6617 0.5
0.0262 0.6976 1
0.1052 0.8928 1
0.0506 0.3105 1
0.0513 0.3132 1
0.0276 0.5367 1
0.0246 0.6454 1
0.4196 0.3181 1
0.0247 0.6591 0.5
0.2046 0.2578 1
0.0273 0.33 1
0.0494 0.3229 1
0.0204 0.4164 1
0.0541 0.6713 1
0.0987 0.7873 1
0.0503 0.5757 1
0.0576 0.3883 1
0.0278 0.2792 1
0.0739 0.4023 1
0.1506 0.8362 1
0.026 0.3061 1
0.1019 0.4044 0.649
0.0259 0.3096 1
0.0503 0.6257 1
0.1068 0.6423 1
0.0244 0.3237 1
0.1019 0.4044 0.649
0.3225 0.3926 1
0.2342 0.2778 1
0.025 0.5181 1
0.0484 0.6019 1
0.0246 0.3899 1
0.1295 0.8124 1
0.0536 0.3887 1
0.025 0.6169 1
0.0242 0.636 1
0.0057307 0.467403 0
0.201 0.4435 1
0.0548 0.3346 0.8133
0.0267 0.5408 1
0.0762 0.435 1
0.0543 0.3897 1
0.1028 0.6279 1
0.0245 0.517 1
0.0268 0.4101 1
0.0489 0.3058 1
0.0104819 0.5 0.5
0.026 0.4669 1
0.1015 0.6218 1
0.0268 0.3715 1
0.0282 0.253 0.828
0.0514 0.3897 1
0.0255 0.2733 1
0.1253 0.7921 1
0.0485 0.3903 1
0.0276 0.5367 1
0.0244 0.6454 1
0.027 0.6859 1
0.0523 0.3116 1
0.0488 0.3885 1
0.025 0.5573 1
0.0742 0.5854 1
0.0266 0.759 1
0.0801 0.4786 1
0.0502 0.3903 1
0.0507 0.3683 1
0.0754 0.7301 0.5
0.0245 0.4212 0
0.0535 0.4455 0.5
0.0494 0.3229 1
0.0511 0.3056 1
0.0572 0.304 1
0.0986 0.3806 1
0.0273 0.33 1
0.0499 0.7566 1
0.0769 0.4569 1
0.0262 0.7549 1
0.0244 0.2623 1
0.0774 0.4733 1
0.0244 0.5344 1
0.1041 0.8937 0.5
0.0248 0.2809 0
0.0516 0.5702 0.5
0.0507 0.3695 1
0.0533 0.6713 1
0.0511 0.3897 1
0.0511 0.3088 1
0.0511 0.5833 1
0.0521 0.2626 1
0.0748 0.9212 1
0.0248 0.5963 0.5
0.029 0.4669 1
0.0286 0.4669 1
0.0576 0.3897 1
0.0512 0.3106 1
0.0572 0.304 1
0.0528 0.3897 1
0.0268 0.5357 1
0.0509 0.3903 1
0.0247 0.2845 1
0.0243 0.5363 1
0.201 0.4435 1
0.0503 0.2863 1
0.0511 0.3897 1
0.0498 0.3903 1
0.0514 0.3897 1
0.0285 0.3565 1
0.0776 0.6728 1
0.1539 0.835 1
0.0251 0.6795 1
0.0273 0.33 1
0.0776 0.4695 1
0.0245 0.3726 1
0.0268 0.4101 1
0.1029 0.8632 1
0.0767 0.626 1
0.0249 0.5258 1
0.011006 1 1
0.0273 0.3003 0.9161
0.1011 0.3896 1
0.0502 0.3037 1
0.0057307 0.467403 0
0.0273 0.613 1
0.0259 0.4622 1
0.134 0.8274 1
0.1333 0.833 1
0.0281 0.3878 0.5
0.0548 0.3346 0.8133
0.0756 0.4402 1
0.0274 0.5494 1
0.0254 0.3492 0.5
0.0245 0.3237 1
0.0274 0.3748 1
0.0283 0.6309 1
0.025 0.272 1
0.0489 0.2828 1
0.0249 0.4524 1
0.0521 0.3897 1
0.0215231 0.542714 0
0.0767 0.626 1
0.0525 0.3891 1
0.0551 0.3897 1
0.0267 0.2662 1
0.0262 0.6924 1
0.0266 0.5195 0
0.0801 0.4786 1
0.0532 0.3891 1
0.025 0.502 1
0.0511 0.5128 1
0.1018 0.4725 1
0.0333514 1 1
0.025 0.6095 0.5
0.0525 0.2662 1
0.0252 0.6454 1
0.0515 0.3105 1
0.0515 0.3105 1
0.0785 0.4819 1
0.0987 0.7873 1
0.0547 0.3897 1
0.0817 0.6765 0.5
0.0528 0.3887 1
0.2342 0.2778 1
0.0784 0.4415 1
0.0502 0.2505 1
0.0501 0.3042 1
0.1539 0.835 1
0.0548 0.3346 0.8133
0.054 0.3897 1
0.0515 0.3087 1
0.0561 0.3361 1
0.025 0.2576 1
0.0301 0.3229 1
0.075 0.4225 1
0.0491 0.4136 1
0.0512 0.3066 1
0.0242 0.3111 1
0.228 0.8644 1
0.0243 0.6094 1
0.0183 0.7443 1
0.0705 0.7301 0.5
0.0285 0.5132 0.5
0.0286 0.55 1
0.0251 0.6472 1
0.0745 0.5809 1
0.4196 0.3181 1
0.0572 0.304 1
0.0120977 0.307771 0
0.201 0.4435 1
0.0512 0.2551 1
0.2586 0.531 1
0.0301 0.3229 1
0.0514 0.3897 1
0.0638 0.5349 1
0.055 0.5714 1
0.0241 0.6377 1
0.1065 0.4308 1
0.1028 0.6333 1
0.0282 0.253 0.828
0.0301 0.3459 1
0.3225 0.3926 1
0.0607 0.2985 1
0.0249 0.3825 1
0.0771 0.3786 1
0.0512 0.3066 1
0.0764 0.3165 1
0.0528 0.3887 1
0.0262 0.463 1
0.0551 0.3897 1
0.0256 0.258 1
0.0256 0.4517 1
0.0261 0.4005 0.4596
0.0105325 1 0.5
0.1018 0.4725 1
0.0491 0.3903 1
0.1278 0.8286 1
0.0263 0.3923 1
0.2586 0.531 1
0.0509 0.2817 1
0.0521 0.3891 1
0.0764 0.3641 1
0.0120977 0.307771 0
0.0519 0.3044 1
0.0242 0.3363 1
0.0491 0.3903 1
0.0526 0.3418 1
0.0252 0.6489 1
0.078 0.3936 1
0.0277 0.592 1
0.0764 0.3165 1
0.0249 0.3066 1
0.025 0.3238 1
0.0508 0.2985 1
0.0244 0.7361 1
0.0245 0.5071 1
0.0515 0.3105 1
0.025 0.4601 1
0.0521 0.3051 1
0.0521 0.3136 1
0.0257 0.5444 1
0.0241 0.3399 1
0.0248 0.4626 0.7308
0.0281 0.2708 1
0.3297 0.2635 0.5058
0.0735 0.5024 0.9326
0.0503 0.5757 1
0.0554 0.2986 0.8805
0.0244 0.272 1
0.1295 0.8124 1
0.1024 0.6186 1
0.0243 0.2744 1
0.0248 0.272 1
0.0242 0.3701 1
0.0245 0.3212 1
0.201 0.4435 1
0.02 0.7361 1
0.0246 0.6454 1
0.0986 0.3806 1
0.0748 0.4662 1
0.026 0.4462 1
0.0512 0.3066 1
0.0519 0.4632 1
0.0521 0.3923 1
0.0136 0.3205 1
0.0527 0.3892 1
0.0245 0.3839 1
0.051 0.3903 1
0.0257 0.4666 1
0.1539 0.835 1
0.0248 0.2743 1
0.0516 0.5702 0.5
0.0498 0.2759 1
0.0779 0.4679 1
0.3225 0.3926 1
0.1019 0.4044 0.649
0.0523 0.9747 1
0.0776 0.4693 1
0.0243 0.6628 1
0.1554 0.6028 1
0.0705 0.7301 0.5
0.0247 0.2845 1
0.0246 0.6089 0.5
0.0288 0.6728 1
0.153 0.8355 1
0.0576 0.3883 1
0.0249 0.6454 1
0.0509 0.3891 1
0.026 0.4497 0.4202
0.0242 0.6094 0.5
0.0503 0.3563 1
0.011006 1 1
0.0269 0.7605 1
0.0523 0.5928 1
0.2342 0.2778 1
0.0762 0.2969 1
0.0499 0.7566 1
0.0486 0.859 1
0.0285 0.3565 1
0.1011 0.3896 1
0.0248 0.5071 1
0.0248 0.2809 0
0.0494 0.4957 1
0.182 0.9826 1
0.2586 0.531 1
0.0244 0.3391 1
0.0244 0.6413 0.5
0.0242 0.2867 1
0.0248 0.6454 1
0.0764 0.3165 1
0.058 0.3885 1
0.0333514 1 1
0.0762 0.3956 1
0.0769 0.6336 1
0.0242 0.3363 1
0.0495 0.3897 1
0.0564 0.6717 1
0.0762 0.435 1
0.0426 0.5359 1
0.2342 0.2778 1
0.3425 0.2739 1
0.0519 0.3187 1
0.0242 0.4317 1
0.0293 0.6312 1
0.0536 0.3891 1
0.0504 0.3897 1
0.0251 0.3749 1
0.0502 0.2505 1
0.0277 0.592 1
0.0248 0.4618 0.8105
0.049 0.9743 1
0.0762 0.2969 1
0.0273 0.531 0.5
0.0786 0.4506 1
0.025 0.4495 1
0.0301 0.3459 1
0.0502 0.2914 1
0.0502 0.6423 0.5
0.0252 0.3643 1
0.0268 0.3715 1
0.0247 0.5803 1
0.0735 0.528 1
0.0246 0.3569 1
0.0796 0.628 0.5
0.0286 0.4669 1
0.0269 0.428 1
0.0284 0.7905 1
0.0509 0.2817 1
0.0136 0.3205 1
0.0246 0.2707 1
0.05 0.3481 1
0.0548 0.3346 0.8133

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EHI_177540 (Entamoeba histolytica), thymidine kinase, putative
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