Detailed view for EHI_185410

Basic information

TDR Targets ID: 797900
Entamoeba histolytica, 26S protease regulatory subunit, putative
Source Database / ID:  AmoebaDB  

pI: 8.2245 | Length (AA): 398 | MW (Da): 44973 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)

Gene Ontology

Mouse over links to read term descriptions.
GO:0036402   GO:proteasome-activating ATPase activity  

GO:0005737   cytoplasm  
GO:0016787   hydrolase activity  
GO:0005524   ATP binding  
GO:0030163   protein catabolic process  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 397 5c19 (D) 350 758 30.00 0.19 1 1.25234 0.63
130 386 3h4m (A) 159 415 58.00 0 1 1.41953 -1.12
136 389 3whk (A) 176 429 56.00 0 1 1.36849 -1.3
302 387 2krk (A) 1 86 69.00 0.044 1 1.06998 -0.96

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127606)

Species Accession Gene Product
Arabidopsis thaliana AT5G20000   AAA-type ATPase family protein
Arabidopsis thaliana AT5G19990   regulatory particle triple-A ATPase 6A
Babesia bovis BBOV_I000480   tat-binding protein-like protein
Brugia malayi Bm1_21785   26S protease regulatory subunit 8
Candida albicans CaO19.11075   likely ATPase similar to S. cerevisiae RPT6 (YGL048C) ATPase subunit of the 19S regulatory particle of the 26S proteasome
Candida albicans CaO19.3593   likely ATPase similar to S. cerevisiae RPT6 (YGL048C) ATPase subunit of the 19S regulatory particle of the 26S proteasome
Caenorhabditis elegans CELE_F56F11.4   Protein F56F11.4, isoform A
Caenorhabditis elegans CELE_Y49E10.1   Protein RPT-6
Cryptosporidium hominis Chro.60119   hypothetical protein
Cryptosporidium parvum cgd6_920   26s protease regulatory subunit 8, putative
Dictyostelium discoideum DDB_G0292382   26S protease regulatory subunit 8
Drosophila melanogaster Dmel_CG2241   Regulatory particle triple-A ATPase 6-related
Drosophila melanogaster Dmel_CG1489   Regulatory particle triple-A ATPase 6
Echinococcus granulosus EgrG_001187600   26S protease regulatory subunit 8
Entamoeba histolytica EHI_185410   26S protease regulatory subunit, putative
Entamoeba histolytica EHI_187600   26S protease regulatory subunit, putative
Echinococcus multilocularis EmuJ_001187600   26S protease regulatory subunit 8
Giardia lamblia GL50803_17106   26S protease regulatory subunit 8
Homo sapiens ENSG00000087191   proteasome (prosome, macropain) 26S subunit, ATPase, 5
Leishmania braziliensis LbrM.35.4600   proteasome regulatory ATPase subunit
Leishmania donovani LdBPK_364570.1   Regulatory particle triple-A ATPase subunit 6
Leishmania infantum LinJ.36.4570   proteasome regulatory ATPase subunit
Leishmania major LmjF.36.4360   proteasome regulatory ATPase subunit
Leishmania mexicana LmxM.36.4360   proteasome regulatory ATPase subunit
Loa Loa (eye worm) LOAG_02984   26S protease regulatory subunit 8
Mus musculus ENSMUSG00000020708   protease (prosome, macropain) 26S subunit, ATPase 5
Neospora caninum NCLIV_026270   26S proteasome regulatory subunit S4 like AAA ATpase, related
Oryza sativa 4328674   Os02g0205300
Oryza sativa 4341448   Os06g0600100
Onchocerca volvulus OVOC7975   26S protease regulatory subunit 8 homolog
Plasmodium berghei PBANKA_1461800   26S protease regulatory subunit 8, putative
Plasmodium falciparum PF3D7_1248900   26S protease regulatory subunit 8, putative
Plasmodium knowlesi PKNH_1468600   26S protease regulatory subunit 8, putative
Plasmodium vivax PVX_101335   26S protease regulatory subunit 8, putative
Plasmodium yoelii PY02897   tat-binding protein homolog
Saccharomyces cerevisiae YGL048C   proteasome regulatory particle base subunit RPT6
Schistosoma japonicum Sjp_0068730   ko:K03066 26S proteasome regulatory subunit T6, putative
Schistosoma mansoni Smp_119310   26S protease regulatory subunit
Schmidtea mediterranea mk4.004327.00   26S protease regulatory subunit 8
Schmidtea mediterranea mk4.023703.02   26S protease regulatory subunit 8
Schmidtea mediterranea mk4.003117.03   26S protease regulatory subunit 8
Schmidtea mediterranea mk4.020129.00   26S protease regulatory subunit 8
Schmidtea mediterranea mk4.002234.02  
Schmidtea mediterranea mk4.037429.00   26S protease regulatory subunit 8
Schmidtea mediterranea mk4.001687.01   26S protease regulatory subunit 8
Schmidtea mediterranea mk4.013659.00   26S protease regulatory subunit 8
Schmidtea mediterranea mk4.003577.07   26S protease regulatory subunit 8
Schmidtea mediterranea mk4.022508.00   26S protease regulatory subunit 8
Schmidtea mediterranea mk4.003143.05   26S protease regulatory subunit 8
Trypanosoma brucei gambiense Tbg972.10.11910   proteasome regulatory ATPase subunit 6,19S proteasome regulatory subunit
Trypanosoma brucei Tb927.10.9740   Regulatory particle triple-A ATPase subunit 6
Trypanosoma congolense TcIL3000_10_8610   Regulatory particle triple-A ATPase subunit 6
Trypanosoma cruzi TcCLB.504147.200   19S proteasome regulatory subunit
Toxoplasma gondii TGME49_261010   tat-binding family protein, putative
Theileria parva TP02_0893   26S proteasome regulatory subunit, putative
Trichomonas vaginalis TVAG_477530   26S protease regulatory subunit S10b, putative

Essentiality

EHI_185410 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9740 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.9740 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9740 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.9740 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y49E10.1 Caenorhabditis elegans embryonic arrest wormbase
CELE_Y49E10.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y49E10.1 Caenorhabditis elegans larval arrest wormbase
CELE_Y49E10.1 Caenorhabditis elegans slow growth wormbase
CELE_F56F11.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F56F11.4 Caenorhabditis elegans larval arrest wormbase
CELE_F56F11.4 Caenorhabditis elegans larval lethal wormbase
CELE_F56F11.4 Caenorhabditis elegans sterile wormbase
YGL048C Saccharomyces cerevisiae inviable yeastgenome
TGME49_261010 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 5 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier EHI_185410 (Entamoeba histolytica), 26S protease regulatory subunit, putative
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