Detailed view for EHI_121880

Basic information

TDR Targets ID: 798691
Entamoeba histolytica, protein kinase domain containing protein

Source Database / ID:  AmoebaDB  

pI: 7.9694 | Length (AA): 382 | MW (Da): 43568 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 370 3lij (A) 40 368 23.00 0 1 1.06398 0.4
13 347 4e7w (A) 18 353 42.00 0 1 1.42806 -0.6
29 346 1j1b (A) 52 371 44.00 0 1 1.41756 -0.74
32 175 3cok (A) 10 154 19.00 0 0.64 0.564363 0.16

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_126888)

Species Accession Gene Product
Arabidopsis thaliana AT3G61160   shaggy-related protein kinase beta
Arabidopsis thaliana AT2G30980   Shaggy-related protein kinase zeta
Arabidopsis thaliana AT3G05840   Shaggy-related protein kinase gamma
Arabidopsis thaliana AT1G57870   shaggy-related protein kinase delta
Arabidopsis thaliana AT4G00720   Shaggy-related protein kinase theta
Arabidopsis thaliana AT5G14640   Shaggy-related protein kinase 13
Arabidopsis thaliana AT1G09840   Shaggy-related protein kinase kappa
Arabidopsis thaliana AT4G18710   Shaggy-related protein kinase eta
Arabidopsis thaliana AT1G06390   shaggy-related protein kinase iota
Arabidopsis thaliana AT5G26751   Shaggy-related protein kinase alpha
Babesia bovis BBOV_III003600   protein kinase domain containing protein
Brugia malayi Bm1_49835   intracellular kinase
Candida albicans CaO19.8410   second truncated form of RIM11 Serine/threonine protein kinase
Candida albicans CaO19.1594   hypothetical protein
Candida albicans CaO19.1593   Serine/threonine protein kinase required for induction of IME2 by Ime1p
Candida albicans CaO19.791   second truncated form of RIM11 Serine/threonine protein kinase
Caenorhabditis elegans CELE_Y18D10A.5   Protein GSK-3
Cryptosporidium hominis Chro.40038   hypothetical protein
Cryptosporidium parvum cgd4_240   hypothetical protein
Dictyostelium discoideum DDB_G0270218   GSK family protein kinase
Dictyostelium discoideum DDB_G0272110   GSK family protein kinase
Drosophila melanogaster Dmel_CG2621   shaggy
Drosophila melanogaster Dmel_CG31003   gasket
Echinococcus granulosus EgrG_000980300   glycogen synthase kinase 3 beta
Echinococcus granulosus EgrG_000352700   protein kinase shaggy
Entamoeba histolytica EHI_003900   protein kinase domain containing protein
Entamoeba histolytica EHI_121880   protein kinase domain containing protein
Entamoeba histolytica EHI_148220   protein kinase, putative
Echinococcus multilocularis EmuJ_000980300   glycogen synthase kinase 3 beta
Echinococcus multilocularis EmuJ_000352700   protein kinase shaggy
Giardia lamblia GL50803_9116   Kinase, CMGC GSK
Giardia lamblia GL50803_17625   Kinase, CMGC GSK
Homo sapiens ENSG00000105723   glycogen synthase kinase 3 alpha
Homo sapiens ENSG00000082701   glycogen synthase kinase 3 beta
Leishmania braziliensis LbrM.18.0290   protein kinase, putative,glycogen synthase kinase, putative
Leishmania donovani LdBPK_180270.1   glycogen synthase kinase 3, putative
Leishmania infantum LinJ.18.0270   glycogen synthase kinase 3
Leishmania major LmjF.18.0270   glycogen synthase kinase, putative;with=GeneDB:LinJ18_V3.0270
Leishmania mexicana LmxM.18.0270   protein kinase, putative,glycogen synthase kinase, putative
Loa Loa (eye worm) LOAG_09639   CMGC/GSK protein kinase
Loa Loa (eye worm) LOAG_03082   CMGC/GSK protein kinase
Mus musculus ENSMUSG00000057177   glycogen synthase kinase 3 alpha
Mus musculus ensembl-mmu:ENSMUSG00000022812   glycogen synthase kinase 3 beta
Neospora caninum NCLIV_039940   hypothetical protein
Oryza sativa 4334738   Os03g0841800
Oryza sativa 4338079   Os05g0207500
Oryza sativa 4327108   Os01g0252100
Oryza sativa 4349147   Os10g0521700
Oryza sativa 4337722   Os05g0134000
Oryza sativa 4341251   Os06g0547900
Oryza sativa 4328830   Os02g0236200
Oryza sativa 9268935   Os03g0679300
Oryza sativa 4326720   Os01g0296100
Onchocerca volvulus OVOC3893  
Plasmodium berghei PBANKA_0410400   glycogen synthase kinase 3, putative
Plasmodium falciparum PF3D7_0312400   glycogen synthase kinase 3
Plasmodium knowlesi PKNH_0829800   glycogen synthase kinase 3, putative
Plasmodium vivax PVX_119725   glycogen synthase kinase 3, putative
Plasmodium yoelii PY01719   Protein kinase domain, putative
Saccharomyces cerevisiae YDL079C   Mrk1p
Saccharomyces cerevisiae YMR139W   Rim11p
Schistosoma japonicum Sjp_0026020   ko:K08822 glycogen synthase kinase 3 alpha, putative
Schistosoma mansoni Smp_008260   glycogen synthase kinase 3-related (gsk3) (cmgc group III)
Schmidtea mediterranea mk4.029485.00  
Schmidtea mediterranea mk4.020670.01   Putative glycogen synthase kinase 3-related
Schmidtea mediterranea mk4.007751.00   Putative glycogen synthase kinase 3-related
Schmidtea mediterranea mk4.007159.01   Glycogen synthase kinase 3
Schmidtea mediterranea mk4.028540.00   Putative glycogen synthase kinase 3-related
Schmidtea mediterranea mk4.004470.03   Putative glycogen synthase kinase 3-related
Schmidtea mediterranea mk4.019543.00  
Schmidtea mediterranea mk4.000777.01  
Schmidtea mediterranea mk4.036542.00  
Schmidtea mediterranea mk4.012060.00   Glycogen synthase kinase 3
Trypanosoma brucei gambiense Tbg972.10.16930   protein kinase, putative,glycogen synthase kinase, putative
Trypanosoma brucei Tb11.v5.0718   protein kinase, putative
Trypanosoma congolense TcIL3000_10_11890   protein kinase, putative
Trypanosoma cruzi TcCLB.507993.80   glycogen synthase kinase 3, putative
Toxoplasma gondii TGME49_265330   cell-cycle-associated protein kinase GSK, putative
Theileria parva TP03_0047   glycogen synthase kinase, putative
Trichomonas vaginalis TVAG_046830   CMGC family protein kinase
Trichomonas vaginalis TVAG_214690   CMGC family protein kinase

Essentiality

EHI_121880 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.13780 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.13780 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.13780 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.13780 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y18D10A.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y18D10A.5 Caenorhabditis elegans larval lethal wormbase
CELE_Y18D10A.5 Caenorhabditis elegans slow growth wormbase
CELE_Y18D10A.5 Caenorhabditis elegans sterile wormbase
PBANKA_0410400 Plasmodium berghei Dispensable plasmo
TGME49_265330 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Sus scrofa Glycogen synthase kinase 3 beta Compounds References
Bos taurus Glycogen synthase kinase-3 beta splice variant X1 Compounds References
Rattus norvegicus Glycogen synthase kinase-3 beta Compounds References
Homo sapiens glycogen synthase kinase 3 alpha Compounds References
Sus scrofa Glycogen synthase kinase-3 Compounds References
Dictyostelium discoideum GSK family protein kinase Compounds References
Plasmodium falciparum glycogen synthase kinase 3 Compounds References
Homo sapiens glycogen synthase kinase 3 beta Compounds References
Mus musculus glycogen synthase kinase 3 alpha Compounds References
Mus musculus glycogen synthase kinase 3 beta Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cell division protein kinase 5 292 aa 34.3% 245 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 26.8% 299 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 30.0% 300 aa Compounds References
Zea mays Casein kinase II alpha 332 aa 28.2% 308 aa Compounds References
Patiria pectinifera Cdc2 300 aa 28.7% 307 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 31.3% 291 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 30.9% 162 aa Compounds References

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0448 0.4177 0.4169
0.0058 1 0.5
0.011 0.2826 1
0.0104 0.5 0.5
0.0292 0.5 0.5
0.175 0.2764 1
0.068 0.5059 0.5105
0.0977 0.5248 1
0.1676 1 1
0.0542 0.2631 0.4786
0.0096 0.3127 1
0.0464 0.2579 1
0.134 1 1
0.0193 0.623 1
0.0191 1 0.5
0.0475 0.2857 1
0.2131 0.7792 1
0.0255 0.3689 1
0.022 0.6958 1
0.0344 1 0.5
0.0116 0.5 0.5
0.0327822 0.649254 1
0.2249 1 1
0.1286 1 1
0.0811 0.2849 0.4168
0.0059 1 1
0.0156 1 1
0.0069 0.3067 0.5
0.0117 1 1
0.0105135 0.5 0.5
0.0059 1 1
0.0059 1 1
0.0224 0.3216 1
0.0118 1 1
0.13 0.2571 1
0.0303 0.5 0.5
0.1578 0.2667 1
0.2366 0.911 1
0.0441 1 0.5
0.1578 0.4068 1
0.0955 0.5386 1
0.207 0.7454 1
0.0016 0.5 0.5
0.00810706 0.5 0.5
0.0113 1 1
0.0059 0.7356 0.7356
0.0204 0.7074 1
0.1578 0.2667 1
0.1429 0.8962 1
0.153 0.5947 1
0.074758 0.259635 0.799584
0.0444 1 1
0.0143 0.3337 0.5
0.0527 0.2874 1
0.1601 0.2769 1
0.0657 1 1
0.0485 0.5339 1
0.2131 0.7792 1
0.064 0.3499 0.9308
0.0541 0.6423 0.5
0.0741 1 1
0.1315 1 1
0.0112 1 1
0.1005 0.5631 1
0.0051 0.4525 0.4525
0.0058 0.307 0.5
0.0547 0.3542 1
0.2679 0.5314 0.8229
0.0689 0.4186 1
0.0165 0.4345 1
0.0863 0.2509 1
0.0312 0.3611 1
0.0431 1 1
0.0193 0.3486 1
0.0155 0.6221 1
0.0098 0.3918 0
0.0107 1 1
0.058 1 1
0.1601 0.2769 1
0.1855 1 1
0.1252 0.2992 0.4681
0.0469 0.504 1
0.0777 0.2672 1
0.13 0.2571 1
0.0751 1 0.5
0.1322 1 1
0.0209 0.4236 1
0.0117 0.3648 0.9415
0.0313 0.6302 0.6298
0.0733 1 1
0.0263 0.6931 1
0.0058 0.5 0.5
0.0117 1 1
0.0294 0.5639 1
0.1787 1 1
0.1174 0.3515 1
0.0455 1 0.5
0.01 1 1
0.0058 1 0.5
0.0022 0.5 0.5
0.0457 1 1
0.149 1 1
0.0303 0.3512 0.5
0.0058 1 0.5
0.0689 1 1
0.0199 0.3116 1
0.1578 0.2667 1
0.0351 0.7179 1
0.0938 1 1
0.0152 0.5873 0.5
0.106 0.8057 1
0.0325 0.2727 0.6978
0.0097 0.3394 0.9404
0.0752 0.6249 0.6228
0.0058 0.5 0.5
0.0117 0.3349 1
0.0459 0.8834 1
0.0478 0.5 0.5
0.0222 0.2523 1
0.2036 0.8481 1
0.0855 0.5 0.5
0.0396 0.3546 0.7506
0.0059 1 1
0.0088 0.4477 0.5
0.0016 0.5 0.5
0.1486 1 1
0.0644 0.3555 1
0.0116 0.5 0.5
0.1578 0.2667 1
0.0116 0.5 0.5
0.1476 1 1
0.0615 0.7728 0.5
0.036 0.5 0.5
0.0373 0.3632 0.8831
0.1242 1 1
0.0721 0.5 0.5
0.0058 0.5 0.5
0.1591 0.9902 1
0.2151 1 1
0.1625 0.7902 0.5
0.0734 0.5 0.5
0.0058 0.5 0.5
0.0407 0.3263 0.6327
0.0293 1 0.5
0.0296 0.5 0.5
0.0252 0.6268 0.5
0.0581 0.8658 0.5
0.017 0.4503 0.6587
0.1426 0.4341 1
0.0581 1 1
0.0059 1 1
0.0116 0.2562 0.1798
0.0632 0.5 0.5
0.0689 1 1
0.0058 1 1
0.1083 0.5 0.5
0.1912 0.4238 1
0.0059 1 1
0.0143 0.3801 1
0.021 0.7277 0.5
0.1498 0.723 1
0.2092 1 1
0.025 0.4293 1
0.0116 0.6659 0.5
0.0117 1 1
0.0055 0.4058 0.4053
0.0059 0.4058 0.4053
0.0117 1 1
0.0118 1 1
0.13 0.2571 1
0.0532 0.3746 1
0.0251 0.3539 0.5153
0.0105 1 1
0.0408 0.5 0.5
0.1578 0.4068 1
0.0054 1 0.5
0.0058 0.3195 0.3053
0.0441 0.482 1
0.0261 0.7953 1
0.1712 1 0.5
0.0157 0.73 1
0.0049 1 1
0.0058 0.5 0.5
0.0402 1 1
0.0303 0.3776 1
0.0358 0.3521 1
0.0058 0.5 0.5
0.3145 1 1
0.1313 1 1
0.1174 0.3515 1
0.0206 1 1
0.1019 1 0.5
0.0687 0.9821 0.982
0.0106 0.5 0.5
0.0058 0.5 0.5
0.0379 0.5 0.5
0.0385 0.8949 0.5
0.0296 1 1
0.1675 0.7177 1
0.1715 0.2825 0.512
0.1492 1 1
0.0117 0.305 0.4231
0.0151 0.3009 0.5
0.0058 0.3954 1
0.1102 0.664 1
0.0733 0.4464 1
0.0119 0.2714 1
0.0515 1 1
0.0744 0.7081 1
0.0346 1 0.5
0.0099 0.5 0.5
0.0674 0.6201 1
0.0342 0.9309 1
0.0589 0.3494 1
0.1022 0.2643 0.4842
0.0395 0.6749 0.5185
0.0053 1 0.5
0.0116 0.5 0.5
0.0802 0.5036 0.5028
0.1578 0.2667 1
0.2168 0.348 0.5234
0.0686 1 1
0.0405 0.8276 1
0.0657 1 1
0.0429 1 0.5
0.1 0.2894 0.8338
0.1578 0.2667 1
0.0525 0.6273 1
0.1578 0.2667 1
0.1578 0.2667 1
0.0642 0.3913 0.5
0.1313 1 1
0.0111 0.5 0.5
0.0058 1 1
0.035 0.5 0.5
0.0887 1 1
0.0107 0.5 0.5
0.0259 0.3976 0.7705
0.1498 0.723 1
0.0104 0.5 0.5
0.1652 0.34 1
0.13 0.2571 1
0.0145 0.2906 0.2906
0.02 0.6614 1
0.1069 0.3643 1
0.0791 0.2705 1
0.162 0.6111 1
0.0467 1 1
0.0097 0.3686 1
0.0395 0.4632 1
0.0117 0.6916 1
0.1601 0.2769 1
0.0727 1 1
0.0835 0.8865 1
0.0191 0.9293 1
0.1847 1 1
0.0116 0.5 0.5
0.0059 0.3111 0.4042
0.1339 1 1
0.0102 0.4498 0.6583
0.0093 0.8828 0
0.0249 0.2962 1
0.14 0.4836 0.8764
0.1185 0.3217 0.4907
0.0202 0.7338 1
0.0106 0.4499 0.6583
0.1088 0.3873 0.4208
0.0378 0.5 0.5
0.0522 0.2706 1
0.243 1 1
0.2647 0.3697 0.5522
0.0369 0.3396 0.4785
0.0598 0.3535 1
0.2201 1 1
0.0118 0.2826 1
0.1632 0.7683 1
0.0576 1 1
0.0146 0.3349 0.6057
0.0117 1 1
0.1519 0.2872 1
0.0053 0.5 0.5
0.0543 1 1
0.0117 0.4663 1
0.0519 0.6326 1
0.0601 1 1
0.0106 0.5398 0.5398
0.025 0.5 0.5
0.0047 0.7658 1
0.0316 1 1
0.0522 0.2925 1
0.0234 0.7796 1
0.0058 0.5 0.5
0.0542 0.2631 0.4786
0.0798 0.511 0.5105
0.0298 0.3042 0.4766
0.0056 1 0.5
0.0117 0.5017 1
0.0481 0.2775 1
0.0059 1 1
0.0213274 0.347055 1
0.0057 0.4071 0.4071
0.1609 1 1
0.1306 1 1
0.162 0.6111 1
0.0855 0.7026 1
0.1071 1 1
0.13 0.2571 1
0.0059 1 1
0.0049 0.2643 1
0.0116 1 0.5
0.2036 0.8481 1
0.0715 0.2698 0.377
0.1511 0.6837 1
0.0612 1 0.5
0.0059 1 1
0.2697 1 1
0.0833 1 1
0.1325 1 1
0.0805 1 1
0.0109 0.4427 0.8477
0.0583165 0.404864 0.404864
0.0802 0.5036 0.5028
0.196 1 1
0.0729 0.7663 1
0.0058 1 0.5
0.0058 0.5 0.5
0.0052 0.5988 1
0.0657 1 1
0.011 0.4408 1
0.0443 0.2664 1
0.1072 0.4185 1
0.0673 0.2601 1
0.049 0.5222 1
0.1578 0.2667 1
0.0644 0.3555 1
0.0491 0.5 0.5
0.0685 0.4332 1
0.011 1 1
0.0464 1 0.5
0.0253 1 1
0.1565 1 1
0.0478 1 1
0.0444 0.523 1
0.0058 0.2621 0.5
0.0358 1 1
0.162 0.6111 1
0.1182 1 1
0.0222 0.2523 1
0.0883 1 1
0.1617 0.3124 1
0.0415 0.4774 0.4773
0.0595 0.3706 1
0.1329 1 1
0.0345 1 1
0.0937 0.6032 1
0.0049 0.296 1
0.0954 0.974 1
0.0116 0.5 0.5
0.1784 1 1
0.0891 0.5278 1
0.0346 0.565 1
0.156255 1 1
0.152 0.5 0.5
0.0791 0.2705 1
0.1493 1 1
0.0235 0.7591 1
0.0569 0.4201 1
0.0153 0.5 0.5
0.0058 0.2925 0.5
0.025 0.6289 1
0.1578 0.2667 1
0.022 1 1
0.0261 0.8455 1
0.0177 0.7835 1
0.0106 1 1
0.123 0.5 0.5
0.0356 1 0.5
0.1191 0.3394 1
0.0056 0.5 0.5
0.0206 1 1
0.2069 0.3129 0.487
0.0782 0.5 0.5
0.1171 0.3452 1
0.0725 1 1
0.0937 0.6032 1
0.0103 0.5 0.5
0.0251348 0.293373 0.647984
0.0861 0.5 0.5
0.1224 1 1
0.0037 1 0.5
0.1592 1 1
0.1532 1 1
0.1992 1 1
0.0053 0.5 0.5
0.0736 0.7648 1
0.1394 0.3222 1
0.0584 1 1
0.1342 0.4886 1
0.0159 0.5363 0.5387
0.0704 1 0.5
0.0887 0.4916 1
0.1408 0.6392 1
0.0756 0.3033 0.5718
0.1652 0.34 1
0.1476 1 1
0.0606 1 1
0.0098 0.3242 0.3167
0.0198 0.2829 1
0.0058 0.5 0.5
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