Detailed view for EHI_115720

Basic information

TDR Targets ID: 798923
Entamoeba histolytica, metallo-beta-lactamase superfamily protein
Source Database / ID:  AmoebaDB  

pI: 5.5263 | Length (AA): 621 | MW (Da): 71015 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00753   Metallo-beta-lactamase superfamily
PF14863   Alkyl sulfatase dimerisation
PF14864   Alkyl sulfatase C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0046983   protein dimerization activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 621 2cfu (A) 26 652 37.00 0 1 1.46669 -0.56
2 621 4nur (A) 48 674 38.00 0 1 1.48669 -0.65
2 621 4pdx (A) 36 660 39.00 0 1 1.46069 -0.52
424 480 1w3b (A) 15 71 26.00 0 0.98 0.495087 -0.78
508 609 2qzt (A) 0 105 25.00 0 0.97 0.567551 -1.38

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_133324)

Species Accession Gene Product
Escherichia coli b4083   metallo-beta-lactamase superfamily protein
Entamoeba histolytica EHI_115720   metallo-beta-lactamase superfamily protein
Entamoeba histolytica EHI_107070   metallo-beta-lactamase superfamily protein
Mycobacterium tuberculosis Rv3762c   Possible hydrolase
Mycobacterium ulcerans MUL_4388   hydrolase
Mycobacterium ulcerans MUL_4362   hydrolase
Mycobacterium ulcerans MUL_3429   hydrolase
Neospora caninum NCLIV_021610   alkyl sulfatase, putative
Saccharomyces cerevisiae YOL164W   Bds1p
Toxoplasma gondii TGME49_203340   metallo-beta-lactamase domain-containing protein

Essentiality

EHI_115720 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b4083 Escherichia coli non-essential goodall
TGME49_203340 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0153 1 1
0.0284 0.6112 1
0.0051 0.5023 0.5
0.0151 0.5 0.5
0.0333 0.487 1
0.0161 1 1
0.0098 0.5 0.5
0.0173 0.5058 1
0.0019 0.5 0.5
0.0139 0.3313 1
0.0068 0.616 0.5
0.0141 0.5 0.5
0.0033 0.5 0.5
0.0263 0.5703 1
0.03 0.9089 1
0.0224 1 1
0.0304 0.3722 1
0.0307 0.4131 1
0.0304 0.3722 1
0.0215 0.5175 0.5
0.0183 1 1
0.0101 0.5 0.5
0.0208 0.4509 1
0.0079 0.301 0
0.0088 0.4106 0.5
0.0065 0.5 0.5
0.0101 0.5 0.5
0.0031 0.5 0.5
0.0249 0.3365 1
0.0085 0.271 1
0.03 0.9089 1
0.0115 1 0.5
0.0367 0.531 1
0.0404 0.2792 1
0.0156 1 1
0.0138 0.8072 0
0.035 0.2506 1
0.024 0.347 0.5
0.0286 1 0.5
0.0259 0.336 1
0.0153 1 1
0.0134 0.3422 1
0.032 0.4025 1
0.0282 0.3876 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_115720 (Entamoeba histolytica), metallo-beta-lactamase superfamily protein
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