Detailed view for EHI_082530

Basic information

TDR Targets ID: 799718
Entamoeba histolytica, acetyl-coA carboxylase, putative
Source Database / ID:  AmoebaDB  

pI: 6.7169 | Length (AA): 1244 | MW (Da): 138015 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00364   Biotin-requiring enzyme
PF00682   HMGL-like
PF01039   Carboxyl transferase domain
PF02436   Conserved carboxylase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0009317   acetyl-CoA carboxylase complex  
GO:0016874   ligase activity  
GO:0003989   acetyl-CoA carboxylase activity  
GO:0003824   catalytic activity  
GO:0006633   fatty acid biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 482 1on3 (A) 36 512 19.00 0 1 0.55406 0.36
244 510 2f9y (A) 61 317 38.00 0 1 0.71623 -0.69
469 1236 2qf7 (A) 398 1151 39.00 0 1 1.07206 -0.42
494 1237 5vz0 (A) 402 1136 41.00 0 1 1.11567 -0.45
622 1080 2nx9 (A) 5 453 33.00 0 1 0.788571 -0.77
1170 1202 2jku (A) 635 667 45.00 0 0.5 0.418127 0.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_126626)

Species Accession Gene Product
Arabidopsis thaliana AT1G03090   methylcrotonoyl-CoA carboxylase subunit alpha
Arabidopsis thaliana AT5G35360   biotin carboxylase subunit CAC2
Candida albicans CaO19.789   likely pyruate carboxylase similar to S. cerevisiae PYC2 (YBR218C) and to Pichia angusta PYC (AAL69566) involved in assembly of
Candida albicans CaO19.780   likely multifunctional enzyme similar to S. cerevisiae DUR1,2 (YBR208C) urea carboxylase and allophanate hydrolase
Candida albicans CaO19.8408   likely pyruate carboxylase similar to S. cerevisiae PYC2 (YBR218C) and to Pichia angusta PYC (AAL69566) involved in assembly of
Candida albicans CaO19.8402   likely multifunctional enzyme similar to S. cerevisiae DUR1,2 (YBR208C) urea carboxylase and allophanate hydrolase
Caenorhabditis elegans CELE_D2023.2   Protein PYC-1, isoform B
Caenorhabditis elegans CELE_F32B6.12   Protein MCCC-1
Caenorhabditis elegans CELE_F27D9.5   Protein PCCA-1
Chlamydia trachomatis CT_124   biotin carboxylase
Dictyostelium discoideum DDB_G0287377   3-methylcrotonyl-CoA:carbon dioxide ligase alpha subunit
Dictyostelium discoideum DDB_G0275355   propanoyl-CoA:carbon dioxide ligase alpha subunit
Drosophila melanogaster Dmel_CG2118   CG2118 gene product from transcript CG2118-RC
Drosophila melanogaster Dmel_CG1516   CG1516 gene product from transcript CG1516-RE
Escherichia coli b3256   acetyl-CoA carboxylase, biotin carboxylase subunit
Echinococcus granulosus EgrG_000534100   propionyl coenzyme A carboxylase alpha chain
Entamoeba histolytica EHI_082530   acetyl-coA carboxylase, putative
Echinococcus multilocularis EmuJ_000534100   propionyl coenzyme A carboxylase alpha chain
Giardia lamblia GL50803_113021   Acetyl-CoA carboxylase/pyruvate carboxylase fusion protein, putative
Homo sapiens ENSG00000175198   propionyl CoA carboxylase, alpha polypeptide
Homo sapiens ENSG00000173599   pyruvate carboxylase
Homo sapiens ENSG00000078070   methylcrotonoyl-CoA carboxylase 1 (alpha)
Leishmania braziliensis LbrM.31.3570   methylcrotonoyl-coa carboxylase biotinylated subunitprotein-like protein
Leishmania braziliensis LbrM.01.0080   carboxylase, putative
Leishmania donovani LdBPK_010050.1   carboxylase, putative
Leishmania donovani LdBPK_313260.1   methylcrotonoyl-coa carboxylase biotinylated subunitprotein-like protein
Leishmania infantum LinJ.01.0050   carboxylase, putative
Leishmania infantum LinJ.31.3260   methylcrotonoyl-coa carboxylase biotinylated subunitprotein-like protein
Leishmania major LmjF.01.0050   carboxylase, putative
Leishmania major LmjF.31.3130   methylcrotonoyl-coa carboxylase biotinylated subunitprotein-like protein
Leishmania mexicana LmxM.01.0050   carboxylase, putative
Leishmania mexicana LmxM.30.3130   methylcrotonoyl-coa carboxylase biotinylated subunitprotein-like protein
Mycobacterium leprae ML0726c   Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase, alpha chain AccA3 (BccP)
Mus musculus ENSMUSG00000027709   methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
Mus musculus ENSMUSG00000024892   pyruvate carboxylase
Mus musculus ENSMUSG00000041650   propionyl-Coenzyme A carboxylase, alpha polypeptide
Mycobacterium tuberculosis Rv2501c   Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA1: biotin carboxylase + biotin carboxyl carrie
Mycobacterium tuberculosis Rv2967c   Probable pyruvate carboxylase Pca (pyruvic carboxylase)
Mycobacterium ulcerans MUL_1992   pyruvate carboxylase
Mycobacterium ulcerans MUL_3779   acetyl-/propionyl-coenzyme a carboxylase alpha chain AccA1
Neospora caninum NCLIV_028990   hypothetical protein
Oryza sativa 4352741   Os12g0605800
Saccharomyces cerevisiae YGL062W   pyruvate carboxylase 1
Saccharomyces cerevisiae YBR208C   bifunctional urea carboxylase/allophanate hydrolase
Saccharomyces cerevisiae YBR218C   pyruvate carboxylase 2
Schistosoma japonicum Sjp_0089840   ko:K01965 propionyl-CoA carboxylase alpha chain [EC6.4.1.3A], putative
Schistosoma japonicum Sjp_0089850   IPR000571,Zinc finger, CCCH-type,domain-containing
Schistosoma japonicum Sjp_0056500   ko:K01959 pyruvate carboxylase subunit A [EC6.4.1.1A], putative
Schistosoma mansoni Smp_165400.3   methylcrotonyl-CoA carboxylase
Schistosoma mansoni Smp_141360   pyruvate carboxylase
Schistosoma mansoni Smp_165400.1   methylcrotonyl-CoA carboxylase
Schmidtea mediterranea mk4.005708.02   Pyruvate carboxylase 1
Schmidtea mediterranea mk4.002747.03  
Schmidtea mediterranea mk4.001068.01  
Schmidtea mediterranea mk4.003313.02   Pyruvate carboxylase 1
Schmidtea mediterranea mk4.003948.02   Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
Schmidtea mediterranea mk4.000717.00   Propionyl-CoA carboxylase alpha chain, mitochondrial
Schmidtea mediterranea mk4.004740.01  
Schmidtea mediterranea mk4.003948.01  
Schmidtea mediterranea mk4.003313.03   Pyruvate carboxylase 1
Trypanosoma brucei gambiense Tbg972.8.7180   3-methylcrotonyl-CoA carboxylase, putative
Trypanosoma brucei Tb927.8.6970   3-methylcrotonyl-CoA carboxylase alpha subunit, putative
Trypanosoma congolense TcIL3000_0_12080   3-methylcrotonyl-CoA carboxylase alpha subunit, putative
Trypanosoma congolense TcIL3000_0_34810   3-methylcrotonyl-CoA carboxylase alpha subunit, putative
Trypanosoma congolense TcIL3000_8_6930   3-methylcrotonyl-CoA carboxylase alpha subunit, putative
Trypanosoma cruzi TcCLB.504835.20   3-methylcrotonyl-CoA carboxylase, putative
Trypanosoma cruzi TcCLB.509913.10   3-methylcrotonyl-CoA carboxylase, putative
Toxoplasma gondii TGME49_284190   pyruvate carboxylase
Treponema pallidum TP0056   pyruvate carboxylase subunit B
Wolbachia endosymbiont of Brugia malayi Wbm0455   Acetyl/propionyl-CoA carboxylase, alpha subunit

Essentiality

EHI_082530 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6970 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.6970 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.6970 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.6970 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3256 Escherichia coli essential goodall
TGME49_284190 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EHI_082530 (Entamoeba histolytica), acetyl-coA carboxylase, putative
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