Detailed view for EHI_100480

Basic information

TDR Targets ID: 801572
Entamoeba histolytica, MIR domain protein
Source Database / ID:  AmoebaDB  

pI: 6.6815 | Length (AA): 211 | MW (Da): 23450 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02815   MIR domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
27 204 1t9f (A) 0 197 39.00 0 1 1.3619 -0.6
33 209 3mal (A) 37 214 45.00 0 1 1.44986 -0.91

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_129318)

Species Accession Gene Product
Arabidopsis thaliana AT2G25110   stromal cell-derived factor 2-like protein
Brugia malayi Bm1_50165   MIR domain containing protein
Caenorhabditis elegans CELE_R12E2.13   Protein R12E2.13
Cryptosporidium hominis Chro.80250   hypothetical protein
Cryptosporidium parvum cgd8_2110   conserved hypothetical protein
Dictyostelium discoideum DDB_G0284847   hypothetical protein
Drosophila melanogaster Dmel_CG11999   CG11999 gene product from transcript CG11999-RA
Echinococcus granulosus EgrG_001176500   stromal cell derived factor 2
Entamoeba histolytica EHI_100480   MIR domain protein
Echinococcus multilocularis EmuJ_001176500   stromal cell derived factor 2
Homo sapiens ENSG00000132581   stromal cell-derived factor 2
Homo sapiens ENSG00000128228   stromal cell-derived factor 2-like 1
Loa Loa (eye worm) LOAG_06469   MIR domain-containing protein
Mus musculus ENSMUSG00000002064   stromal cell derived factor 2
Mus musculus ENSMUSG00000022769   stromal cell-derived factor 2-like 1
Neospora caninum NCLIV_044950   hypothetical protein, conserved
Oryza sativa 4345684   Os08g0440500
Oryza sativa 4345160   Os08g0278900
Onchocerca volvulus OVOC517  
Plasmodium berghei PBANKA_1209100   dolichyl-phosphate-mannose protein mannosyltransferase, putative
Plasmodium falciparum PF3D7_1010700   dolichyl-phosphate-mannose protein mannosyltransferase, putative
Plasmodium knowlesi PKNH_0810500   dolichyl-phosphate-mannose protein mannosyltransferase, putative
Plasmodium vivax PVX_094755   hypothetical protein, conserved
Plasmodium yoelii PY02090   hypothetical protein
Schistosoma japonicum Sjp_0002550   Stromal cell-derived factor 2 precursor, putative
Schistosoma mansoni Smp_170920   stromal cell-derived factor 2 precursor-like protein
Schmidtea mediterranea mk4.034732.00   Stromal cell-derived factor 2-like protein
Toxoplasma gondii TGME49_228630   hypothetical protein
Trichomonas vaginalis TVAG_455070   mannosyltransferase, putative
Trichomonas vaginalis TVAG_494110   mannosyltransferase, putative
Trichomonas vaginalis TVAG_028970   mannosyltransferase 1, putative

Essentiality

EHI_100480 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_228630 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0443 0.3388 0.5
0.0125 0.2722 1
0.0443 0.3388 0.5
0.0127 0.3029 1
0.0443 0.3388 0.5
0.0446 0.3501 1
0.0443 0.3388 0.5
0.0443 0.3388 0.5
0.0076 0.3079 1
0.0676 0.3614 1
0.0716 0.357 1
0.0653878 0.251143 0.5
0.061 0.3626 1
0.0443 0.3388 0.5
0.0443 0.3388 0.5
0.0443 0.2595 0.7279

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_100480 (Entamoeba histolytica), MIR domain protein
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