Detailed view for EHI_183030

Basic information

TDR Targets ID: 801887
Entamoeba histolytica, translation initiation factor 4e, putative
Source Database / ID:  AmoebaDB  

pI: 4.7997 | Length (AA): 183 | MW (Da): 21315 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01652   Eukaryotic initiation factor 4E

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0003723   RNA binding  
GO:0003743   translation initiation factor activity  
GO:0006413   translational initiation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 179 5abx (A) 31 210 24.00 0 1 1.36836 -1.33
7 183 2wmc (A) 52 225 24.00 0 1 1.37521 -1.08
7 177 2jgb (A) 53 218 30.00 0 1 1.38803 -1.31
7 145 5nvn (A) 53 188 34.00 0 1 1.15406 -0.91

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_130916)

Species Accession Gene Product
Babesia bovis BBOV_III011150   translation initiation factor E4, putative
Dictyostelium discoideum DDB_G0270406   eukaryotic translation initiation factor 4E member 3
Drosophila melanogaster Dmel_CG1442   CG1442 gene product from transcript CG1442-RA
Echinococcus granulosus EgrG_000261200   eukaryotic translation initiation factor 4E type
Entamoeba histolytica EHI_183030   translation initiation factor 4e, putative
Homo sapiens ENSG00000163412   eukaryotic translation initiation factor 4E family member 3
Leishmania braziliensis LbrM.30.0530   eukaryotic translation initiation factor 4e, putative
Leishmania donovani LdBPK_300460.1   Eukaryotic translation initiation factor 4E-4
Leishmania infantum LinJ.30.0460   eukaryotic translation initiation factor 4e, putative
Leishmania major LmjF.30.0450   eukaryotic translation initiation factor 4e, putative
Leishmania mexicana LmxM.29.0450   eukaryotic translation initiation factor 4e, putative
Mus musculus ENSMUSG00000093661   eukaryotic translation initiation factor 4E member 3
Plasmodium berghei PBANKA_0412800   eukaryotic translation initiation factor 4E, putative
Plasmodium falciparum PF3D7_0315100   eukaryotic translation initiation factor 4E
Plasmodium knowlesi PKNH_0827000   eukaryotic translation initiation factor 4E, putative
Plasmodium vivax PVX_095480   translation initiation factor 4E, putative
Plasmodium yoelii PY04859   putative initiation factor E4
Trypanosoma brucei gambiense Tbg972.6.1570   eukaryotic translation initiation factor 4e, putative
Trypanosoma brucei Tb927.6.1870   Eukaryotic translation initiation factor 4E-4
Trypanosoma congolense TcIL3000_6_1470   Eukaryotic translation initiation factor 4E-4
Trypanosoma cruzi TcCLB.509037.40   Eukaryotic translation initiation factor 4E-4
Trypanosoma cruzi TcCLB.421959.10   Eukaryotic translation initiation factor 4E-4
Trichomonas vaginalis TVAG_108990   eukaryotic translation initiation factor 4E, putative

Essentiality

EHI_183030 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1870 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1870 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.1870 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1870 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_183030 (Entamoeba histolytica), translation initiation factor 4e, putative
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