Detailed view for EHI_015550

Basic information

TDR Targets ID: 803270
Entamoeba histolytica, retroviral aspartyl protease domain-containing protein
Source Database / ID:  AmoebaDB  

pI: 5.9725 | Length (AA): 239 | MW (Da): 27127 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF09668   Aspartyl protease

Gene Ontology

Mouse over links to read term descriptions.
GO:0043130   ubiquitin binding  
GO:0004190   aspartic-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128104)

Species Accession Gene Product
Arabidopsis thaliana AT3G13235   DNA damage-inducible protein 1
Babesia bovis BBOV_III010190   conserved hypothetical protein
Brugia malayi Bm1_32555   UBA/TS-N domain containing protein
Candida albicans CaO19.7258   similar to S. cerevisiae DNA Damage Inducible protein that binds to T- and V- snare complexes
Caenorhabditis elegans CELE_C01G5.6   Protein C01G5.6
Cryptosporidium hominis Chro.30259   hypothetical protein
Cryptosporidium parvum cgd3_2190   ubiquitin domain containing protein with a UBA domain at the C-terminus
Dictyostelium discoideum DDB_G0288187   ubiquitin-associated domain-containing protein
Drosophila melanogaster Dmel_CG4420   rings lost
Echinococcus granulosus EgrG_000898400   protein ddi1 2
Entamoeba histolytica EHI_095840   retroviral aspartyl protease domain-containing protein
Entamoeba histolytica EHI_015550   retroviral aspartyl protease domain-containing protein
Echinococcus multilocularis EmuJ_000898400   protein ddi1 2
Giardia lamblia GL50803_16228   DNA-damage inducible protein DDI1-like
Giardia lamblia GL50803_7718   DNA-damage inducible protein DDI1-like
Homo sapiens ENSG00000170967   DNA-damage inducible 1 homolog 1 (S. cerevisiae)
Homo sapiens 84301   DNA-damage inducible 1 homolog 2 (S. cerevisiae)
Leishmania braziliensis LbrM.01.0030   DNA-damage inducible protein DDI1-like protein
Leishmania donovani LdBPK_010630.1   DNA-damage inducible protein DDI1-like protein
Leishmania infantum LinJ.01.0630   DNA-damage inducible protein DDI1-like protein
Leishmania major LmjF.01.0610   DNA-damage inducible protein DDI1-like protein
Leishmania mexicana LmxM.01.0610   DNA-damage inducible protein DDI1-like protein
Loa Loa (eye worm) LOAG_09845   UBA/TS-N domain-containing protein
Loa Loa (eye worm) LOAG_04248   hypothetical protein
Mus musculus 68817   DNA-damage inducible protein 2
Mus musculus ENSMUSG00000047619   DDI1, DNA-damage inducible 1, homolog 1 (S. cerevisiae)
Neospora caninum NCLIV_044460   DNA-damage inducible protein, putative
Oryza sativa 4328632   Os02g0198600
Onchocerca volvulus OVOC3056  
Plasmodium berghei PBANKA_1033200   DNA damage-inducible protein 1, putative
Plasmodium falciparum PF3D7_1409300   DNA damage-inducible protein 1, putative
Plasmodium knowlesi PKNH_1349100   DNA damage-inducible protein 1, putative
Plasmodium vivax PVX_085985   DNA damage-inducible protein 1, putative
Plasmodium yoelii PY06672   hypothetical protein
Saccharomyces cerevisiae YER143W   Ddi1p
Schistosoma japonicum Sjp_0202880   Protein DDI1 homolog 2, putative
Schistosoma mansoni Smp_063670   hypothetical protein
Schmidtea mediterranea mk4.006892.00   Protein DDI1 homolog 2
Schmidtea mediterranea mk4.002510.04   Protein DDI1 homolog 2
Schmidtea mediterranea mk4.018112.01   Protein DDI1 homolog 2
Trypanosoma brucei gambiense Tbg972.9.2320   DNA-damage inducible protein DDI1-like protein, putative
Trypanosoma brucei Tb927.9.4390   DNA-damage inducible protein DDI1-like protein
Trypanosoma congolense TcIL3000_9_1440   DNA-damage inducible protein DDI1-like protein
Trypanosoma cruzi TcCLB.511585.40   DNA-damage inducible protein DDI1-like protein, putative
Trypanosoma cruzi TcCLB.510155.40   DNA-damage inducible protein DDI1-like protein, putative
Toxoplasma gondii TGME49_304680   ubiquitin family protein
Theileria parva TP02_0129   hypothetical protein
Trichomonas vaginalis TVAG_459080   Clan AA, family A2, retrotansposon aspartic peptidase

Essentiality

EHI_015550 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.2990 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.2990 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.2990 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.160.2990 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1033200 Plasmodium berghei Essential plasmo
TGME49_304680 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Human immunodeficiency virus HIV-1 protease 99 aa 31.4% 102 aa Compounds References
Human immunodeficiency virus 1 Protease 99 aa 32.4% 102 aa Compounds References
Human immunodeficiency virus 1 Human immunodeficiency virus type 1 protease 99 aa 31.1% 103 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier EHI_015550 (Entamoeba histolytica), retroviral aspartyl protease domain-containing protein
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