Detailed view for EHI_028910

Basic information

TDR Targets ID: 804547
Entamoeba histolytica, ARF GTPase activating protein, putative
Source Database / ID:  AmoebaDB  

pI: 10.0706 | Length (AA): 260 | MW (Da): 28808 | Paralog Number: 3

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01412   Putative GTPase activating protein for Arf

Gene Ontology

Mouse over links to read term descriptions.
GO:0005096   GTPase activator activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 138 3sub (A) 1 130 38.00 0 1 1.13215 -1.73
9 153 2crw (A) 12 148 40.00 0 1 1.07769 -1.11
10 128 3dwd (A) 3 114 45.00 0 1 0.986992 -0.86

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127813)

Species Accession Gene Product
Arabidopsis thaliana AT3G53710   putative ADP-ribosylation factor GTPase-activating protein AGD6
Arabidopsis thaliana AT2G37550   ADP-ribosylation factor GTPase-activating protein AGD7
Babesia bovis BBOV_IV009590   ADP-ribosylation factor GTPase-activating factor, putative
Brugia malayi Bm1_57440   GTP-ase activating protein for Arf containing protein
Candida albicans CaO19.11167   potential ADP ribosylation factor GTPase activating protein (ARF GAP) similar to S. cerevisiae GCS1 (YDL226C) ARF GAP with a rol
Candida albicans CaO19.3683   potential ADP ribosylation factor GTPase activating protein (ARF GAP) similar to S. cerevisiae GCS1 (YDL226C) ARF GAP with a rol
Caenorhabditis elegans CELE_K02B12.7   Protein K02B12.7
Cryptosporidium hominis Chro.20341   beta 7 subunit of 20S proteasome
Cryptosporidium parvum cgd2_3240   ARF GAP-like zinc finger-containing protein
Dictyostelium discoideum DDB_G0281627   Arf GTPase activating protein
Drosophila melanogaster Dmel_CG4237   ADP-ribosylation factor GTPase activating protein 1
Echinococcus granulosus EgrG_000605500   ADP ribosylation factor GTPase activating
Entamoeba histolytica EHI_004810   hypothetical protein, conserved
Entamoeba histolytica EHI_189140   ARF GTPase activating protein, putative
Entamoeba histolytica EHI_069440   ARF GTPase activating protein, putative
Entamoeba histolytica EHI_028910   ARF GTPase activating protein, putative
Echinococcus multilocularis EmuJ_000605500   ADP ribosylation factor GTPase activating
Homo sapiens ENSG00000101199   ADP-ribosylation factor GTPase activating protein 1
Leishmania braziliensis LbrM.32.1380   ADP-ribosylation factor GTPase activating protein 1, putative
Leishmania donovani LdBPK_321290.1   ADP-ribosylation factor GTPase activating protein 1, putative
Leishmania infantum LinJ.32.1290   ADP-ribosylation factor GTPase activating protein 1, putative
Leishmania major LmjF.32.1230   ADP-ribosylation factor GTPase activating protein 1, putative
Leishmania mexicana LmxM.31.1230   ADP-ribosylation factor GTPase activating protein 1, putative
Loa Loa (eye worm) LOAG_05511   GTP-ase activating protein for Arf containing protein
Mus musculus ENSMUSG00000027575   ADP-ribosylation factor GTPase activating protein 1
Neospora caninum NCLIV_047400   hypothetical protein
Oryza sativa 4332427   Os03g0278400
Onchocerca volvulus OVOC436  
Plasmodium berghei PBANKA_1457800   ADP-ribosylation factor GTPase-activating protein, putative
Plasmodium falciparum PF3D7_1244600   ADP-ribosylation factor GTPase-activating protein, putative
Plasmodium knowlesi PKNH_1464300   ADP-ribosylation factor GTPase-activating protein, putative
Plasmodium vivax PVX_101125   GTP-ase activating protein for Arf containing protein
Plasmodium yoelii PY03828   ADP-ribosylation factor GTPase-activating protein
Saccharomyces cerevisiae YDL226C   Gcs1p
Schistosoma japonicum Sjp_0012680   ADP-ribosylation factor GTPase-activating protein 1, putative
Schistosoma mansoni Smp_152370   arf gtpase-activating protein
Trypanosoma brucei gambiense Tbg972.11.16200   ADP-ribosylation factor GTPase activating protein 1, putative
Trypanosoma brucei Tb927.11.14460   ADP-ribosylation factor GTPase activating protein 1, putative
Trypanosoma congolense TcIL3000.11.14750   ADP-ribosylation factor GTPase activating protein 1, putative
Trypanosoma cruzi TcCLB.506871.30   ADP-ribosylation factor GTPase activating protein 1, putative
Trypanosoma cruzi TcCLB.507507.20   ADP-ribosylation factor GTPase activating protein 1, putative
Toxoplasma gondii TGME49_225310   ARF1-directed GTPase-activating protein, putative
Theileria parva TP01_0810   ADP-ribosylation factor GTPase-activating protein, putative
Trichomonas vaginalis TVAG_183110   arf-GTPase activating protein, putative

Essentiality

EHI_028910 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.6060 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.6060 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.6060 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.6060 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_K02B12.7 Caenorhabditis elegans slow growth wormbase
TGME49_225310 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus ADP-ribosylation factor GTPase-activating protein 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.003 0.6315 0.8005

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier EHI_028910 (Entamoeba histolytica), ARF GTPase activating protein, putative
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