Detailed view for Rv1627c

Basic information

TDR Targets ID: 8046
Mycobacterium tuberculosis, Probable nonspecific lipid-transfer protein

Source Database / ID:  Tuberculist 

pI: 6.0958 | Length (AA): 402 | MW (Da): 42386 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00108   Thiolase, N-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016747   transferase activity, transferring groups other than amino-acyl groups  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 402 4e1l (A) 0 392 23.00 0.074 1 0.894637 0.8
9 400 6et9 (A) 4 385 34.00 0 1 1.31322 -0.06
9 400 4yzo (A) 2 368 27.00 0 1 1.15332 -0.15
23 115 4v2p (A) 55 148 26.00 0.056 1 0.470543 -0.26
33 346 4u4e (A) 34 323 35.00 0 1 0.905195 1.08

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase. hasan
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_146299)

Species Accession Gene Product
Leishmania braziliensis LbrM.15.0270   nonspecific lipid-transfer protein, putative,sterol carrier protein, putative
Leishmania donovani LdBPK_150280.1   nonspecific lipid-transfer protein, putative
Leishmania infantum LinJ.15.0280   nonspecific lipid-transfer protein, putative,sterol carrier protein, putative
Leishmania major LmjF.15.0240   nonspecific lipid-transfer protein, putative,sterol carrier protein, putative
Leishmania mexicana LmxM.15.0240   nonspecific lipid-transfer protein, putative,sterol carrier protein, putative
Mycobacterium tuberculosis Rv1627c   Probable nonspecific lipid-transfer protein
Trypanosoma brucei gambiense Tbg972.8.1450   nonspecific lipid-transfer protein, putative,sterol carrier protein, putative
Trypanosoma brucei Tb927.8.1820   sterol carrier protein, putative
Trypanosoma congolense TcIL3000_8_1750   sterol carrier protein, putative
Trypanosoma cruzi TcCLB.511393.10   nonspecific lipid-transfer protein, putative

Essentiality

Rv1627c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
this record Mycobacterium tuberculosis non-essential nmpdr
Tb927.8.1820 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.1820 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.1820 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.1820 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv1627c (Mycobacterium tuberculosis), Probable nonspecific lipid-transfer protein
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