Detailed view for EHI_130960

Basic information

TDR Targets ID: 804627
Entamoeba histolytica, purine nucleoside phosphorylase, putative
Source Database / ID:  AmoebaDB  

pI: 6.831 | Length (AA): 238 | MW (Da): 26269 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01048   Phosphorylase superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0006139   nucleobase, nucleoside, nucleotide and nucleic acid metabolic process  
GO:0004731   purine-nucleoside phosphorylase activity  
GO:0003824   catalytic activity  
GO:0009116   nucleoside metabolic process  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 238 3u40 (A) 1 238 99.99 0 1 2.1976 -1.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3TL6:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3U40:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_130516)

Species Accession Gene Product
Babesia bovis BBOV_III005140   phosphorylase family protein
Dictyostelium discoideum DDB_G0284431   uridine phosphorylase
Drosophila melanogaster Dmel_CG17224   CG17224 gene product from transcript CG17224-RC
Escherichia coli b4384   purine-nucleoside phosphorylase
Entamoeba histolytica EHI_130960   purine nucleoside phosphorylase, putative
Entamoeba histolytica EHI_130930   purine nucleoside phosphorylase, putative
Neospora caninum NCLIV_053130   hypothetical protein
Plasmodium berghei PBANKA_1113000   purine nucleoside phosphorylase, putative
Plasmodium falciparum PF3D7_0513300   purine nucleoside phosphorylase
Plasmodium knowlesi PKNH_1019800   purine nucleoside phosphorylase, putative
Plasmodium vivax PVX_080575   purine nucleoside phosphorylase, putative
Plasmodium yoelii PY04622   purine nucleoside phosphorylase
Toxoplasma gondii TGME49_307030   Purine nucleoside phosphorylase
Treponema pallidum TP1027   uridine phosphorylase (udp)
Treponema pallidum TP0734   purine nucleoside phosphorylase (deoD)
Theileria parva TP02_0437   purine nucleoside phosphorylase, putative
Theileria parva TP02_0440   purine nucleoside phosphorylase, putative
Theileria parva TP02_0438   purine nucleoside phosphorylase, putative
Trichomonas vaginalis TVAG_127180   purine nucleoside phosphorylase, putative
Trichomonas vaginalis TVAG_454490   purine nucleoside phosphorylase, putative

Essentiality

EHI_130960 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b4384 Escherichia coli non-essential goodall
PBANKA_1113000 Plasmodium berghei Essential plasmo
TGME49_307030 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum purine nucleoside phosphorylase Compounds References
Toxoplasma gondii Purine nucleoside phosphorylase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 231 aa 28.1% 192 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0719 0.3598 0.5
0.1365 0.3996 1
0.4298 0.383 1
0.0144 0.2545 1
0.0147 0.32 1
0.2536 0.8786 1
0.3965 0.8137 1
0.365 0.825 1
0.4018 0.5238 1
0.0146 1 1
0.4343 0.3029 1
0.07 0.3377 0.5
0.0216 0.285 1
0.0243 1 0.5
0.0152 0.3736 1
0.0153 0.6136 1
0.3872 0.7864 1
0.0319 1 1
0.0211 1 0.5
0.0211 1 0.5
0.4044 0.7351 1
0.0451 0.9877 0.5
0.3627 0.7741 1
0.2536 0.8786 1
0.0197 0.5105 1
0.0921 0.8899 1
0.4428 0.4025 1
0.018 0.5 0.5
0.3872 0.7864 1
0.0261 0.3959 1
0.0164 0.41 1
0.0211 1 0.5
0.4697 0.7926 1
0.3872 0.7864 1
0.0492 0.3293 1
0.2349 0.2613 0.5
0.018 0.7599 0.5
0.0295 0.2611 1
0.365 0.825 1
0.018 0.3007 1
0.1741 0.8258 0.5
0.4412 0.7191 0.5
0.1372 0.8147 0.5
0.196 0.8458 0.5
0.3872 0.7864 1
0.0152 0.3736 1
0.2564 0.2906 0.5
0.0211 1 0.5
0.4058 0.3709 0.5
0.0223 0.2518 1
0.4592 0.802 1
0.2982 0.8108 1
0.4644 0.7912 1
0.4031 0.7833 1
0.0159 0.3007 1
0.0568 0.2609 1
0.3696 0.7306 1
0.018 0.643 1
0.3199 0.7981 1
0.4298 0.383 1
0.016 0.3007 1
0.018 0.3007 1
0.0211 1 0.5
0.4643 0.8049 1
0.018 0.3007 1
0.4298 0.383 1
0.0146 0.3007 1
0.0211 1 0.5
0.1734 0.6451 1
0.0211 1 0.5
0.0330744 0.481619 1
0.0197 0.5105 1
0.0211 1 0.5
0.4311 0.7679 1
0.0167 0.3902 1
0.0211 1 0.5
0.0261 0.3959 1
0.4058 0.3709 0.5
0.3983 0.7961 1
0.0151 1 1
0.4116 0.8263 1
0.4455 0.7578 0.5
0.0211 1 0.5
0.1743 0.8336 1
0.0568 0.2609 1
0.018 0.643 1
0.0243 1 0.5
0.1734 0.6451 1
0.3113 0.8199 1
0.0407 0.2888 1
0.4133 0.8116 1
0.1734 0.6451 1
0.018 0.3007 1
0.0243 1 0.5
0.0789 0.6984 0.5
0.0991 0.8829 1
0.4133 0.8116 1
0.0172 0.7692 1
0.1365 0.3996 1
0.0451 0.9877 0.5
0.0211 1 0.5
0.018 0.3007 1
0.0175 0.3304 1
0.2446 0.806 1
0.0211 1 0.5
0.4298 0.383 1
0.3959 0.8038 1
0.0211 1 0.5
0.047 0.8385 1
0.018 0.3007 1
0.4428 0.4025 1
0.018 0.7599 0.5
0.4695 0.8006 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EHI_130960 (Entamoeba histolytica), purine nucleoside phosphorylase, putative
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