Detailed view for EHI_027710

Basic information

TDR Targets ID: 804672
Entamoeba histolytica, calcium-transporting P-type ATPase, putative
Source Database / ID:  AmoebaDB  

pI: 5.875 | Length (AA): 1137 | MW (Da): 127014 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 10

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00122   E1-E2 ATPase
PF00689   Cation transporting ATPase, C-terminus
PF00690   Cation transporter/ATPase, N-terminus
PF00702   haloacid dehalogenase-like hydrolase
PF13246   Cation transport ATPase (P-type)

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0000166   nucleotide binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
60 125 2qif (A) 2 67 8.00 0.00000016 0 0.183847 -0.68
63 124 1yjr (A) 7 68 13.00 0.000057 0.02 0.192329 -0.28
192 1133 3wgu (A) 23 1016 25.00 0.0000000003 1 1.0253 0.41
204 1130 2zxe (A) 42 1023 29.00 0 1 1.1054 -0.14
321 393 2cw8 (A) 101 163 43.00 0.17 0.15 0.165004 1.31
564 752 1q3i (A) 386 591 21.00 0 1 0.345327 -0.45
748 883 1nnl (A) 86 215 22.00 0 0.63 0.228713 0.48

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128979)

Species Accession Gene Product
Babesia bovis BBOV_IV010020   P-type ATPase4, putative
Candida albicans CaO19.12637   P-type Na+ATPase
Candida albicans CaO19.13491   P-type ATPase, Na+ efflux
Candida albicans CaO19.6070   P-type ATPase, Na+ efflux
Candida albicans CaO19.5170   P-type Na+ATPase
Candida albicans CaO19_5170   hypothetical protein
Cryptosporidium hominis Chro.40306   ATPase
Cryptosporidium parvum cgd4_2720   P-type ATpase involved in cation transport
Entamoeba histolytica EHI_027710   calcium-transporting P-type ATPase, putative
Leishmania braziliensis LbrM.34.1990   calcium motive p-type ATPase, putative
Leishmania donovani LdBPK_352080.1   calcium motive p-type ATPase, putative
Leishmania infantum LinJ.35.2080   calcium motive p-type ATPase, putative
Leishmania major LmjF.35.2080   calcium motive p-type ATPase, putative
Leishmania mexicana LmxM.34.2080   calcium motive p-type ATPase, putative
Neospora caninum NCLIV_067350   P-type Ca(2 )-ATPase, putative
Saccharomyces cerevisiae YDR038C   putative Na(+)-exporting P-type ATPase ENA5
Saccharomyces cerevisiae YDR039C   Na(+)-exporting P-type ATPase ENA2
Saccharomyces cerevisiae YDR040C   Na(+)/Li(+)-exporting P-type ATPase ENA1
Trypanosoma brucei gambiense Tbg972.9.9590   calcium motive p-type ATPase, putative
Trypanosoma brucei Tb927.9.15460   calcium motive p-type ATPase, putative
Trypanosoma cruzi TcCLB.506241.70   calcium motive p-type ATPase, putative
Trypanosoma cruzi TcCLB.510769.120   calcium motive p-type ATPase, putative
Toxoplasma gondii TGME49_278660   P-type ATPase4, putative
Theileria parva TP01_0720   P-type ATPase, putative

Essentiality

EHI_027710 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.244.2570 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.244.2570 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.244.2570 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.244.2570 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_278660 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 1043 aa 31.7% 994 aa Compounds References
Sus scrofa Potassium-transporting ATPase alpha chain 1 1034 aa 29.3% 998 aa Compounds References
Rattus norvegicus Potassium-transporting ATPase 1033 aa 30.4% 901 aa Compounds References
Rattus norvegicus Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 994 aa 30.1% 995 aa Compounds References
Rattus norvegicus Sodium/potassium-transporting ATPase alpha-1 chain 1023 aa 30.7% 981 aa Compounds References
Canis lupus familiaris Sodium/potassium-transporting ATPase alpha-1 chain 1021 aa 30.9% 985 aa Compounds References
Sus scrofa Sodium/potassium-transporting ATPase alpha-1 chain 1021 aa 30.9% 985 aa Compounds References
Oryctolagus cuniculus Sarcoplasmic/endoplasmic reticulum calcium ATP-ase 1001 aa 32.3% 836 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0196 0.4947 1
0.0149 0.5101 1
0.0588 0.6181 1
0.0783 0.3167 1
0.0538 0.5576 1
0.0486 0.6383 1
0.0084 0.355 1
0.0028 0.5312 1
0.0293 0.3322 1
0.0031 0.427 1
0.1072 0.6768 1
0.0673 0.5622 1
0.0166 0.5576 1
0.0096 0.2864 1
0.0379 0.5454 1
0.0793 0.5434 1
0.0105 0.5576 1
0.0059 0.2519 1
0.022 0.3105 1
0.0027 0.2657 1
0.0124 0.5754 1
0.0017 0.3384 1
0.0088 0.5736 1
0.0017 0.3153 1
0.0265 0.3806 1
0.0129 0.3292 1
0.0783 0.3167 1
0.005 0.2898 1
0.0516 0.5329 1
0.0166 0.5576 1
0.0046 0.3659 0.8219
0.0084 0.3968 1
0.0134 0.5576 1
0.0031 0.2864 1
0.0101 0.5736 1
0.0731 0.5435 1
0.097 0.5893 1
0.0217 0.5662 1
0.0038 0.2596 1
0.058 0.5355 1
0.0281 0.6365 1
0.0162 0.6249 1
0.0042 0.5273 1
0.028 0.3981 1
0.0706 0.5471 1
0.0109 0.5754 1
0.084 0.5636 1
0.0017 0.5273 1
0.0949 0.5475 1
0.0115 0.2854 1
0.0213 0.5284 1
0.0289 0.5754 1
0.106 0.5498 1
0.0664 0.5393 1
0.016 0.5736 1
0.0886 0.418 1
0.0738 0.3411 0.34
0.0034 0.3589 1
0.1018 0.2569 1
0.0542 0.6548 1
0.0061 0.5576 0.8199
0.003 0.2595 0.6884
0.0718 0.5419 1
0.0017 0.5273 1
0.0887 0.5487 1
0.0146 0.5736 1
0.0236 0.5576 1
0.0038 0.2596 1
0.0089 0.2657 1
0.0066 0.2851 1
0.0512 0.5476 1
0.0754 0.5433 1
0.047 0.5576 1
0.0996 0.6364 1
0.0801 0.5448 1
0.011 0.5878 1
0.081 0.5147 1
0.0706 0.5471 1
0.0588 0.6181 1
0.0973 0.6118 1
0.0265 0.3806 1
0.0014 0.4606 1
0.0116 0.2657 1
0.0017 0.5576 1
0.0034 0.427 1
0.0792 0.5456 1
0.0059 0.2814 1
0.002 0.427 1
0.0058 0.2814 1
0.0859 0.612 1
0.0481 0.5736 1
0.0327 0.5145 1
0.0017 0.5576 1
0.0244 0.4862 1
0.0846 0.5461 1
0.0244 0.5576 1
0.0037 0.6569 0.6569
0.0231 0.5576 1
0.071 0.5418 1
0.0017 0.3358 0.2964
0.0087 0.2814 1
0.0988 0.5496 1
0.0038 0.427 0.5321
0.0518 0.2515 1
0.0679 0.5409 1
0.0124 0.5754 1
0.0886 0.418 1
0.0413 0.5264 1
0.0017 0.5576 1
0.0413 0.5264 1
0.0409 0.4248 1
0.0085 0.3513 1
0.103 0.5904 1
0.0441 0.5075 0.6719
0.0573 0.2918 1
0.0073 0.2814 1
0.0886 0.418 1
0.0077 0.2657 1
0.0732 0.5416 1
0.0518 0.2515 1
0.0347 0.5576 1
0.0631 0.6195 1
0.0293 0.3322 1
0.0045 0.5576 1
0.0237 0.5736 1
0.0682 0.5406 1
0.0017 0.5736 1
0.0323 0.3385 1
0.0756 0.5863 1
0.0886 0.418 1
0.0801 0.5448 1
0.0206 0.5736 1
0.0038 0.2596 1
0.0034 0.5736 1
0.0414 0.5231 1
0.0355 0.5194 1
0.0119 0.6529 1
0.0204 0.4852 1
0.0886 0.418 1
0.0202 0.5736 1
0.0028 0.2657 1
0.0161 0.305 1
0.0112 0.5736 1
0.0902 0.5467 1
0.0835 0.5865 1
0.0323 0.3385 1
0.0622 0.5379 1
0.0795 0.5861 1
0.0322 0.2826 0.8461
0.0052 0.2561 0.5837
0.0541 0.5273 1
0.0323 0.3385 1
0.0237 0.5736 1
0.006 0.3981 1
0.0083 0.4515 1
0.1292 0.6077 1
0.0941 0.5828 1
0.0867 0.5459 1
0.0094 0.2697 0.8649
0.0196 0.4947 1
0.0888 0.5489 1
0.0622 0.5377 1
0.0113 0.3532 1
0.0396 0.5267 1
0.0664 0.6048 1
0.0089 0.2657 1
0.0038 0.427 1
0.0814 0.5465 1
0.0074 0.5754 1
0.084 0.5636 1
0.0411 0.5226 1
0.0246 0.2961 1
0.0038 0.427 0.713
0.0689 0.5416 1
0.0016 0.427 0.7798
0.0621 0.4909 1
0.0783 0.3167 1
0.0121 0.5736 1
0.0783 0.3171 1
0.0738 0.3411 0.34
0.0162 0.6249 1
0.0398 0.6095 1
0.0887 0.5487 1
0.0043 0.5576 1
0.0657 0.5409 1
0.0149 0.5101 1
0.0914 0.5502 1
0.0074 0.2873 1
0.0017 0.5576 1
0.0029 0.2814 1
0.0445 0.5278 1
0.0105 0.2577 0.81
0.0754 0.5453 1
0.0065 0.3224 1
0.0017 0.5576 1
0.0443 0.5576 1
0.0512 0.5476 1
0.0066 0.5273 1
0.0055 0.2898 1
0.0512 0.5476 1
0.011 0.2577 0.81
0.007 0.5273 1
0.0039 0.2945 1
0.051 0.5576 1
0.0121 0.6131 1
0.0125 0.3064 1
0.0043 0.2814 1
0.0017 0.2657 1
0.0097 0.5676 1
0.0062 0.5576 1
0.0544 0.5361 1
0.0768 0.5781 1
0.0063 0.5921 1
0.081 0.5147 1
0.0248 0.5028 1
0.0512 0.5476 1
0.0045 0.5576 1
0.1018 0.2569 1
0.0215 0.5576 1
0.0078 0.3522 1
0.006 0.5461 1
0.0293 0.3322 1
0.0225 0.5576 1
0.0493 0.5576 1
0.0218 0.6131 1
0.0104 0.2543 1
0.0183 0.4863 0.5179
0.0142 0.5665 1
0.0017 0.4817 1
0.0347 0.5576 1
0.072 0.5443 1
0.0028 0.3869 1
0.0161 0.305 1
0.0096 0.2864 1
0.0902 0.5467 1
0.0738 0.3411 0.34
0.017 0.5576 1
0.0601 0.5799 1
0.032 0.5397 1
0.075 0.543 1
0.0029 0.5576 1
0.0512 0.5476 1
0.0015 0.3067 1
0.0206 0.5736 1
0.0845 0.6024 1
0.0046 0.3659 0.8219
0.0613 0.5369 1
0.0933 0.3759 1
0.0512 0.5476 1
0.0102 0.5736 1
0.0664 0.6048 1
0.0264 0.5736 1
0.0017 0.5576 1
0.0219 0.6131 1
0.0487 0.5317 1
0.0102 0.2599 1
0.0721 0.5416 1
0.0969 0.5892 1
0.0854 0.5453 1
0.0017 0.3736 0
0.0617 0.5396 1
0.0471 0.5576 1
0.0742 0.5433 1
0.0441 0.5075 0.6719
0.0048 0.2795 1
0.0028 0.366 1
0.0233 0.2831 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_027710 (Entamoeba histolytica), calcium-transporting P-type ATPase, putative
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