Detailed view for EHI_174640

Basic information

TDR Targets ID: 805240
Entamoeba histolytica, glucosamine-6-phosphate isomerase, putative
Source Database / ID:  AmoebaDB  

pI: 6.4705 | Length (AA): 672 | MW (Da): 75692 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01182   Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase
PF02585   GlcNAc-PI de-N-acetylase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004342   glucosamine-6-phosphate deaminase activity  
GO:0006044   N-acetylglucosamine metabolic process  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
24 265 2bkx (A) 1 239 42.00 0 1 0.979519 -1.2
24 267 2ri0 (A) 1 233 42.00 0 1 0.881495 -0.49
24 284 1fs5 (A) 1 264 36.00 0 1 0.912793 -0.95
28 317 1ne7 (A) 5 279 38.00 0 1 0.822048 -0.26
362 398 2cjz (A) 291 328 46.00 0.88 0.33 0.43186 0.87
368 625 1q7t (A) 0 252 17.00 0 0.74 0.159329 1.08
382 644 5cgz (A) 5 217 23.00 0.000000000013 0.94 0.387769 0.47
383 666 2ixd (A) 4 220 22.00 0 1 0.295119 0.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128189)

Species Accession Gene Product
Candida albicans CaO19.9703   C. albicans glucosamine-6-phosphate deaminase
Candida albicans CaO19.2156   N terminal truncated version of C. albicans glucosamine-6-phosphate deaminase
Caenorhabditis elegans CELE_T03F6.3   Protein T03F6.3
Dictyostelium discoideum DDB_G0278873   glucosamine-6-phosphate deaminase
Dictyostelium discoideum DDB_G0286195   PIG-L family protein
Drosophila melanogaster Dmel_CG6957   CG6957 gene product from transcript CG6957-RC
Escherichia coli b0678   glucosamine-6-phosphate deaminase
Entamoeba histolytica EHI_174640   glucosamine-6-phosphate isomerase, putative
Giardia lamblia GL50803_8245   Glucosamine-6-phosphate deaminase
Giardia lamblia GL50803_10829   Glucosamine-6-phosphate deaminase
Homo sapiens ENSG00000163281   glucosamine-6-phosphate deaminase 2
Homo sapiens ENSG00000113552   glucosamine-6-phosphate deaminase 1
Leishmania braziliensis LbrM.32.3550   glucosamine-6-phosphate isomerase, putative
Leishmania donovani LdBPK_323460.1   glucosamine-6-phosphate isomerase, putative
Leishmania infantum LinJ.32.3460   glucosamine-6-phosphate isomerase, putative
Leishmania major LmjF.32.3260   glucosamine-6-phosphate isomerase, putative
Leishmania mexicana LmxM.31.3260   glucosamine-6-phosphate isomerase, putative,glucosamine-6-phosphate deaminase, putative
Mus musculus ENSMUSG00000029209   glucosamine-6-phosphate deaminase 2
Mus musculus ENSMUSG00000052102   glucosamine-6-phosphate deaminase 1
Schistosoma japonicum Sjp_0304390   ko:K02564 glucosamine-6-phosphate isomerase, putative
Schistosoma mansoni Smp_091850   glucosamine-6-phosphate isomerase
Schmidtea mediterranea mk4.001705.06   Probable glucosamine-6-phosphate isomerase
Trypanosoma brucei gambiense Tbg972.11.18920   glucosamine-6-phosphate isomerase, putative
Trypanosoma brucei Tb927.11.16770   glucosamine-6-phosphate isomerase, putative
Trypanosoma congolense TcIL3000.11.16680   glucosamine-6-phosphate isomerase, putative
Trypanosoma cruzi TcCLB.511025.50   glucosamine-6-phosphate isomerase, putative
Trypanosoma cruzi TcCLB.511531.50   glucosamine-6-phosphate isomerase, putative
Trichomonas vaginalis TVAG_365520   glucosamine-6-phosphate isomerase, putative

Essentiality

EHI_174640 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.8520 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.8520 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.8520 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.8520 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0678 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EHI_174640 (Entamoeba histolytica), glucosamine-6-phosphate isomerase, putative
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