Detailed view for GL50803_9421

Basic information

TDR Targets ID: 806167
Giardia lamblia, Kinase, NEK
Source Database / ID:  GiardiaDB  

pI: 6.7311 | Length (AA): 584 | MW (Da): 64455 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF12796   Ankyrin repeats (3 copies)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 450 3hx4 (A) 46 481 25.00 0 1 0.950911 0.39
10 207 3nup (A) 13 206 41.00 0.00000000016 0.96 0.457941 0.57
299 572 2fo1 (E) 936 1292 23.00 0.000000000019 1 0.549678 0.84
399 511 5gik (A) 47 165 32.00 0.0015 1 0.624393 -0.81
462 570 5kwd (A) 4 118 38.00 0.00004 1 0.634544 -0.78
465 576 2v5q (C) 18 135 23.00 0 1 0.525281 -0.79
491 583 3t9k (A) 606 702 20.00 0.0027 1 0.509747 -1.16

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_142551)

Species Accession Gene Product
Giardia lamblia GL50803_9421   Kinase, NEK
Giardia lamblia GL50803_9665   Kinase, NEK
Mycobacterium tuberculosis Rv2088   Transmembrane serine/threonine-protein kinase J PknJ (protein kinase J) (STPK J)
Mycobacterium tuberculosis Rv1743   Probable transmembrane serine/threonine-protein kinase E PknE (protein kinase E) (STPK E)
Mycobacterium tuberculosis Rv1266c   Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H)
Mycobacterium tuberculosis Rv2914c   Probable transmembrane serine/threonine-protein kinase I PknI (protein kinase I) (STPK I) (phosphorylase B kinase kinase) (hydro
Mycobacterium ulcerans MUL_3180   Ser/Thr protein kinase
Mycobacterium ulcerans MUL_2042   Ser/Thr protein kinase

Essentiality

GL50803_9421 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1773 Mycobacterium tuberculosis non-essential nmpdr
mtu1287 Mycobacterium tuberculosis non-essential nmpdr
mtu2961 Mycobacterium tuberculosis non-essential nmpdr
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mycobacterium tuberculosis Probable transmembrane serine/threonine-protein kinase H PknH (protein kinase H) (STPK H) Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 25.7% 292 aa Compounds References
Rattus norvegicus Casein kinase I delta 415 aa 21.6% 393 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 26.0% 292 aa Compounds References
Bos taurus Casein kinase I isoform alpha 325 aa 22.3% 291 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 31.9% 163 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 23.4% 265 aa Compounds References
Sus scrofa Casein kinase 1, delta tv1 415 aa 21.9% 393 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 26.8% 306 aa Compounds References
Oryctolagus cuniculus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 25.2% 298 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0004 0.5 0.5
0.0023 0.5 0.5
0.0091 1 0.5
0.0059 1 1
0.0012 0.5 0.5
0.0008 0.5 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0069 0.3067 0.5
0.0027 1 0.5
0.0064 0.3377 0.5
0.0011 1 0.5
0.0093 0.8828 0.5
0.0029 0.5 0.5
0.0022 0.5 0.5
0.0056 1 0.5
0.0081 0.5 0.5
0.0036 0.5 0.5
0.0012 0.5 0.5
0.0026 0.5 0.5
0.0033 0.5 0.5
0.0061 0.6883 1
0.0088 0.4477 0.5
0.0032 0.5 0.5
0.0092 1 0.5
0.0063 0.7244 0
0.0007 0.5 0.5
0.0059 1 1
0.0059 1 1
0.0067 0.5 0.5
0.0081 1 0.5
0.0007 0.5 0.5
0.0062 0.6935 0.5
0.0037 1 0.5
0.0063 1 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2373
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0042 0.5 0.5
0.0007 0.5 0.5
0.0018 0.5 0.5
0.0033 1 0.5
0.0066 0.3101 0.5
0.0003 0.5 0.5
0.0016 0.5 0.5
0.0039 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier GL50803_9421 (Giardia lamblia), Kinase, NEK
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