Detailed view for GL50803_104173

Basic information

TDR Targets ID: 809401
Giardia lamblia, Isoleucyl-tRNA synthetase
Source Database / ID:  GiardiaDB  

pI: 7.7182 | Length (AA): 1165 | MW (Da): 133603 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00133   tRNA synthetases class I (I, L, M and V)
PF08264   Anticodon-binding domain of tRNA

Gene Ontology

Mouse over links to read term descriptions.
GO:0002161   GO:aminoacyl-tRNA editing activity  

GO:0005737   cytoplasm  
GO:0004822   isoleucine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0000049   tRNA binding  
GO:0005524   ATP binding  
GO:0006428   isoleucyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  

Metabolic Pathways

Aminoacyl-tRNA biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 1021 1ffy (A) 6 911 25.00 0 1 1.02632 0.55
20 928 1ile (A) 9 821 40.00 0 1 1.17916 -0.27
735 993 4xkz (A) 632 880 13.00 0.00049 0.78 0.398818 -0.41
911 1130 4tql (A) 9 228 11.00 0.57 0.09 0.313341 -0.38
944 1011 1ng6 (A) 61 135 44.00 0.72 1 0.277269 0.83

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126684)

Species Accession Gene Product
Arabidopsis thaliana AT4G10320   tRNA synthetase class I (I, L, M and V) family protein
Arabidopsis thaliana AT5G49030   isoleucyl-tRNA synthetase-like protein
Babesia bovis BBOV_III006640   isoleucyl-tRNA synthetase family protein
Babesia bovis BBOV_II005760   tRNA synthetases class I protein superfamily
Brugia malayi Bm1_49235   isoleucyl-tRNA synthetase
Brugia malayi Bm1_49970   isoleucyl-tRNA synthetase family protein
Candida albicans CaO19.9685   probable tRNA synthetase similar to S. cerevisiae ILS1 (YBL076C) Isoleucyl-tRNA synthetase, cytoplasmic
Candida albicans CaO19.2382   likely tRNA synthetase similar to S. cerevisiae ISM1 (YPL040C) nuclear encoded mitochondrial tRNA-Ile synthetase
Candida albicans CaO19.2138   probable tRNA synthetase similar to S. cerevisiae ILS1 (YBL076C) Isoleucyl-tRNA synthetase, cytoplasmic
Candida albicans CaO19.9918   likely tRNA synthetase similar to S. cerevisiae ISM1 (YPL040C) nuclear encoded mitochondrial tRNA-Ile synthetase
Caenorhabditis elegans CELE_R11A8.6   Protein IARS-1
Cryptosporidium hominis Chro.30434   isoleucine-tRNA synthetase
Cryptosporidium parvum cgd3_3840   isoleucine-tRNA synthetase
Chlamydia trachomatis CT_019   isoleucine--tRNA ligase
Dictyostelium discoideum DDB_G0278293   isoleucine-tRNA ligase
Dictyostelium discoideum DDB_G0293030   isoleucine-tRNA ligase
Drosophila melanogaster Dmel_CG5414   CG5414 gene product from transcript CG5414-RB
Drosophila melanogaster Dmel_CG11471   Isoleucyl-tRNA synthetase
Escherichia coli b0026   isoleucyl-tRNA synthetase
Echinococcus granulosus EgrG_000754600   isoleucyl trna synthetase mitochondrial
Echinococcus granulosus EgrG_002008700   isoleucyl tRNA synthetase, cytoplasmic
Entamoeba histolytica EHI_177660   isoleucyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_000296500   isoleucyl tRNA synthetase, cytoplasmic
Echinococcus multilocularis EmuJ_000754600   isoleucyl tRNA synthetase, mitochondrial
Giardia lamblia GL50803_104173   Isoleucyl-tRNA synthetase
Homo sapiens ENSG00000196305   isoleucyl-tRNA synthetase
Homo sapiens ENSG00000067704   isoleucyl-tRNA synthetase 2, mitochondrial
Leishmania braziliensis LbrM.35.5900   isoleucyl-tRNA synthetase, putative
Leishmania donovani LdBPK_365870.1   isoleucyl-tRNA synthetase, putative
Leishmania infantum LinJ.36.5870   isoleucyl-tRNA synthetase, putative
Leishmania major LmjF.36.5620   isoleucyl-tRNA synthetase, putative
Leishmania mexicana LmxM.36.5620   isoleucyl-tRNA synthetase, putative
Loa Loa (eye worm) LOAG_12099   hypothetical protein
Loa Loa (eye worm) LOAG_00948   isoleucyl-tRNA synthetase
Loa Loa (eye worm) LOAG_10039   hypothetical protein
Mycobacterium leprae ML1195   isoleucyl-tRNA synthetase IleS
Mus musculus ENSMUSG00000037851   isoleucine-tRNA synthetase
Mus musculus ENSMUSG00000026618   isoleucine-tRNA synthetase 2, mitochondrial
Mycobacterium tuberculosis Rv1536   Isoleucyl-tRNA synthetase IleS
Mycobacterium ulcerans MUL_1532   isoleucyl-tRNA synthetase
Neospora caninum NCLIV_002610   isoleucine-tRNA synthetase, putative
Oryza sativa 9272464   Os02g0183700
Oryza sativa 9268435   Os02g0183900
Oryza sativa 9270869   Os06g0645400
Oryza sativa 4330908   Os02g0778200
Onchocerca volvulus OVOC6300  
Plasmodium berghei PBANKA_1347700   isoleucine--tRNA ligase, putative
Plasmodium falciparum PF3D7_1332900   isoleucine--tRNA ligase, putative
Plasmodium knowlesi PKNH_1267700   isoleucine--tRNA ligase, putative
Plasmodium vivax PVX_082575   isoleucine--tRNA ligase, putative
Plasmodium yoelii PY01849   isoleucyl-tRNA synthetase, putative
Saccharomyces cerevisiae YPL040C   isoleucine--tRNA ligase ISM1
Saccharomyces cerevisiae YBL076C   isoleucine--tRNA ligase ILS1
Schistosoma japonicum Sjp_0009980   ko:K01870 isoleucyl-tRNA synthetase [EC6.1.1.5], putative
Schistosoma japonicum Sjp_0013140   ko:K01870 isoleucyl-tRNA synthetase [EC6.1.1.5], putative
Schistosoma japonicum Sjp_0106740   ko:K01870 isoleucyl-tRNA synthetase [EC6.1.1.5], putative
Schistosoma japonicum Sjp_0105480   ko:K01870 isoleucyl-tRNA synthetase [EC6.1.1.5], putative
Schistosoma mansoni Smp_041600   isoleucine--tRNA ligase
Schistosoma mansoni Smp_129650   isoleucine--tRNA ligase
Schmidtea mediterranea mk4.002309.03  
Schmidtea mediterranea mk4.017819.00  
Schmidtea mediterranea mk4.007235.00  
Schmidtea mediterranea mk4.020820.00   IsoleucineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.061508.00  
Schmidtea mediterranea mk4.002309.02   IsoleucineALQ-tRNA ligase, cytoplasmic
Schmidtea mediterranea mk4.006953.00  
Schmidtea mediterranea mk4.053875.01   IsoleucineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.049669.00  
Schmidtea mediterranea mk4.015797.00  
Schmidtea mediterranea mk4.008013.00  
Schmidtea mediterranea mk4.002309.01   IsoleucineALQ-tRNA ligase, cytoplasmic
Trypanosoma brucei gambiense Tbg972.10.11240   isoleucyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.10.9190   isoleucyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.509797.40   isoleucyl-tRNA synthetase, putative
Toxoplasma gondii TGME49_207640   isoleucyl-tRNA synthetase family protein
Treponema pallidum TP0452   isoleucyl-tRNA synthetase
Theileria parva TP02_0310   isoleucyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_024690   isoleucyl tRNA synthetase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0363   isoleucyl-tRNA synthetase

Essentiality

GL50803_104173 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9190 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.9190 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9190 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.9190 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0026 Escherichia coli essential goodall
CELE_R11A8.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_R11A8.6 Caenorhabditis elegans larval arrest wormbase
CELE_R11A8.6 Caenorhabditis elegans slow growth wormbase
YBL076C Saccharomyces cerevisiae inviable yeastgenome
TGME49_207640 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens isoleucyl-tRNA synthetase Compounds References
Escherichia coli isoleucyl-tRNA synthetase Compounds References
Staphylococcus aureus Isoleucyl-tRNA synthetase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0512 0.361 1
0.0033 0.3584 1
0.0495 0.585 1
0.051 0.3346 1
0.0176 0.3778 1
0.0585 0.3561 1
0.0351 0.4273 1
0.043 0.5997 1
0.0074 0.6073 1
0.0547 0.266 1
0.0495 0.585 1
0.0493 0.3438 1
0.0033 0.3584 1
0.0506 0.27 1
0.0514 0.4686 1
0.0042 0.3607 1
0.0074 0.2908 0.5
0.0511 0.3314 1
0.0183 0.3463 1
0.043 0.5997 1
0.0042 0.3607 1
0.0147 0.4036 1
0.0423 0.3502 1
0.0509 0.2804 1
0.043 0.5997 1
0.0114776 0.471217 0.708495
0.0554 0.4645 1
0.0493 0.3438 1
0.0509 0.265 1
0.0314 0.3765 1
0.0576 0.504 1
0.0547 0.3389 1
0.0203 0.3209 1
0.0119 0.3315 1
0.0514 0.469 1
0.0506 0.27 1
0.0204 0.4035 1
0.0352 0.4199 1
0.0509 0.265 1
0.0506 0.27 1
0.0495 0.4715 1
0.0235 0.3734 1
0.0423 0.3651 1
0.0547 0.3389 1
0.0479 0.3329 1
0.043 0.5997 1
0.0034 1 1
0.0506 0.27 1
0.0178 0.3889 1
0.0508 0.3511 1
0.0524 0.55 1
0.028 0.4702 1
0.0094 0.5339 0.5
0.0218925 1 1
0.0576 0.504 1
0.0146 0.3863 1
0.0425 0.4756 1
0.0465 0.3112 1
0.0059 0.4766 1
0.0547 0.266 1
0.0545 0.309 1
0.0453 0.4702 1
0.0389 0.4258 1
0.0479 0.3329 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier GL50803_104173 (Giardia lamblia), Isoleucyl-tRNA synthetase
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