Detailed view for GL50803_10744

Basic information

TDR Targets ID: 812394
Giardia lamblia, Kinase, NEK
Source Database / ID:  GiardiaDB  

pI: 5.1345 | Length (AA): 1120 | MW (Da): 121993 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00023   Ankyrin repeat
PF00069   Protein kinase domain
PF12796   Ankyrin repeats (3 copies)
PF13637   Ankyrin repeats (many copies)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 16 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
18 215 2a19 (B) 266 483 21.00 0 1 0.273086 -0.07
18 278 2wqm (A) 33 298 21.00 0 1 0.414336 -0.42
24 291 3ka0 (A) 69 346 31.00 0.000061 1 0.373986 0.46
24 458 4ysj (A) 33 460 25.00 0.000046 1 0.507093 0.52
54 325 2i6l (A) 57 300 26.00 0.068 1 0.252557 0.21
54 293 2rku (A) 94 326 24.00 0.086 1 0.420986 -0.26
57 274 5uis (D) 213 456 15.00 0 1 0.282543 -0.35
59 219 4uya (A) 167 343 32.00 0.026 0.61 0.12845 0.87
295 1014 4rlv (A) 2058 2805 30.00 0 1 0.885557 0.28
497 611 2jab (A) 12 132 31.00 0 1 0.532579 -0.99
563 611 4bsz (B) 52 112 49.00 0.23 1 0.48045 -1.06
591 676 2xen (A) 13 102 33.00 0 1 0.554686 -1.22
872 1112 5iwk (A) 36 291 28.00 0.4 1 0.323879 0.82
1033 1119 4k5a (B) 46 136 32.00 0.0000000000064 1 0.564979 -1.58
1035 1117 4hbd (A) 740 827 39.00 0.00000088 1 0.562407 -1.09
1036 1117 2jab (A) 49 134 29.00 0.000000045 1 0.487514 -1.15

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_131598)

Species Accession Gene Product
Brugia malayi Bm1_09655   Ankyrin homolog precursor
Caenorhabditis elegans CELE_T28D6.4   Protein T28D6.4
Drosophila melanogaster Dmel_CG10011   CG10011 gene product from transcript CG10011-RA
Echinococcus granulosus EgrG_001099500   ankyrin repeat domain containing protein 50
Echinococcus granulosus EgrG_001201800   ankyrin domain repeat containing protein
Echinococcus multilocularis EmuJ_001099500   ankyrin repeat domain containing protein 50
Giardia lamblia GL50803_114671   Protein 21.1
Giardia lamblia GL50803_10744   Kinase, NEK
Homo sapiens ENSG00000151458   ankyrin repeat domain 50
Loa Loa (eye worm) LOAG_15521   hypothetical protein
Loa Loa (eye worm) LOAG_12449   hypothetical protein
Loa Loa (eye worm) LOAG_13408   hypothetical protein
Loa Loa (eye worm) LOAG_16031   hypothetical protein
Loa Loa (eye worm) LOAG_14446   hypothetical protein
Oryza sativa 4333611   Os03g0655500
Onchocerca volvulus OVOC9615  
Schistosoma japonicum Sjp_0116550   hypothetical protein
Schistosoma japonicum Sjp_0091200   Ankyrin-1, putative
Schistosoma mansoni Smp_140520.2   ank repeat-containing
Schistosoma mansoni Smp_140520.1   ank repeat-containing
Schmidtea mediterranea mk4.001311.06   Si:dkey-145p14.5 protein
Schmidtea mediterranea mk4.014341.00  
Schmidtea mediterranea mk4.001311.08  
Schmidtea mediterranea mk4.001311.07  

Essentiality

GL50803_10744 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_T28D6.4 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 22.1% 172 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0012 0.5 0.5
0.0091 1 0.5
0.0059 1 1
0.0023 0.5 0.5
0.0004 0.5 0.5
0.0032 0.5 0.5
0.0069 0.3067 0.5
0.0039 0.5 0.5
0.0008 0.5 0.5
0.0093 0.8828 0.5
0.0027 1 0.5
0.0064 0.3377 0.5
0.0011 1 0.5
0.0012 0.5 0.5
0.0036 0.5 0.5
0.0081 0.5 0.5
0.0056 1 0.5
0.0022 0.5 0.5
0.0029 0.5 0.5
0.0032 0.5 0.5
0.0092 1 0.5
0.0063 0.7244 0
0.0088 0.4477 0.5
0.0061 0.6883 1
0.0026 0.5 0.5
0.0033 0.5 0.5
0.0081 1 0.5
0.0007 0.5 0.5
0.0062 0.6935 0.5
0.0067 0.5 0.5
0.0059 1 1
0.0007 0.5 0.5
0.0059 1 1
0.0042 0.5 0.5
0.0016 0.5 0.5
0.0098 0.3242 0.2373
0.0012 0.5 0.5
0.0039 0.9485 0.5
0.0063 1 0.5
0.0037 1 0.5
0.0003 0.5 0.5
0.0066 0.3101 0.5
0.0016 0.5 0.5
0.0039 0.5 0.5
0.0018 0.5 0.5
0.0033 1 0.5
0.0007 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier GL50803_10744 (Giardia lamblia), Kinase, NEK
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