Detailed view for PBANKA_0517600

Basic information

TDR Targets ID: 826385
Plasmodium berghei, plasmepsin VII
Source Database / ID: 

pI: 8.4558 | Length (AA): 471 | MW (Da): 54286 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00026   Eukaryotic aspartyl protease

Gene Ontology

Mouse over links to read term descriptions.
GO:0004190   aspartic-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
46 444 1pfz (A) 86 329 24.00 0 1 0.924534 0.27
58 437 1miq (A) 99 322 30.00 0 1 1.05599 -0.05
88 334 1b5f (A) 6 224 30.00 0.0000000072 1 0.691616 0.07
94 439 4aa9 (A) 14 321 31.00 0 1 0.961807 -0.53
96 176 4od9 (A) 15 92 42.00 0.0000075 0.41 0.488175 0.39

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Gametocyte, 4 hs Ring, 22 hs Schizont, Male gametocyte. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Ookinete, Female gametocyte. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile 16 hs Trophozoite, Erthyrocytic stages. Otto TD Yeoh LM
Show/Hide expression data references
  • Yeoh LM Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates.
  • Otto TD A comprehensive evaluation of rodent malaria parasite genomes and gene expression.

Orthologs

Ortholog group members (OG5_145118)

Species Accession Gene Product
Babesia bovis BBOV_IV009660   aspartyl protease, putative
Neospora caninum NCLIV_034810   eukaryotic aspartyl protease, putative
Plasmodium berghei PBANKA_0517600   plasmepsin VII
Plasmodium falciparum PF3D7_1033800   plasmepsin VII
Plasmodium knowlesi PKNH_0618600   aspartyl protease, putative
Plasmodium vivax PVX_111035   aspartyl protease, putative
Plasmodium yoelii PY00469   Eukaryotic aspartyl protease
Toxoplasma gondii TGME49_272510   aspartyl protease
Theileria parva TP01_0802   hypothetical protein
Theileria parva TP01_0803   hypothetical protein

Essentiality

PBANKA_0517600 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0517600 this record Plasmodium berghei Dispensable plasmo
TGME49_272510 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus Cathepsin D 390 aa 26.0% 377 aa Compounds References
Macaca mulatta Renin 406 aa 26.3% 365 aa Compounds References
Callithrix jacchus Renin 400 aa 26.0% 389 aa Compounds References
Sus scrofa Pepsin A 385 aa 28.5% 410 aa Compounds References
Penicillium janthinellum Penicillopepsin 323 aa 21.6% 347 aa Compounds References
Plasmodium falciparum plasmepsin II 453 aa 25.1% 406 aa Compounds References
Rattus norvegicus Renin 402 aa 27.2% 360 aa Compounds References
Schistosoma mansoni cathepsin D (A01 family) 430 aa 25.8% 360 aa Compounds References
Schistosoma mansoni cathepsin D (A01 family) 428 aa 25.8% 360 aa Compounds References
Macaca fascicularis Renin 406 aa 26.2% 367 aa Compounds References
Rhizopus microsporus var. chinensis Rhizopuspepsin 393 aa 24.1% 349 aa Compounds References
Oryctolagus cuniculus Renin 280 aa 24.9% 325 aa Compounds References
Rattus norvegicus Pepsinogen C 392 aa 27.3% 366 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PBANKA_0517600 (Plasmodium berghei), plasmepsin VII
Title for this comment
Comment