Detailed view for PBANKA_1211900

Basic information

TDR Targets ID: 826781
Plasmodium berghei, phosphoinositide-specific phospholipase C
Source Database / ID: 

pI: 9.207 | Length (AA): 1344 | MW (Da): 159089 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00387   Phosphatidylinositol-specific phospholipase C, Y domain
PF00388   Phosphatidylinositol-specific phospholipase C, X domain
PF12814   Meiotic cell cortex C-terminal pleckstrin homology

Gene Ontology

Mouse over links to read term descriptions.
GO:0035556   GO:intracellular signal transduction  

GO:0032065   cortical protein anchoring  
GO:0005938   cell cortex  
GO:0008081   phosphoric diester hydrolase activity  
GO:0005543   phospholipid binding  
GO:0005515   protein binding  
GO:0004435   phosphoinositide phospholipase C activity  
GO:0007165   signal transduction  
GO:0006629   lipid metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 1229 5xh6 (A) 26 1256 11.00 0 1 0.72549 1.36
286 355 1n0y (B) 76 145 9.00 0.054 0.01 0.139983 -0.15
321 1335 3qr1 (A) 98 767 25.00 0 1 0.184008 2.19
554 1050 3qr0 (A) 297 637 39.00 0 0.97 0.166792 1.33
700 932 1nu7 (D) 30 271 11.00 0 0.05 0.128163 -0.56
1213 1337 2ep6 (A) 99 214 18.00 0.000026 0.26 0.078906 0.92

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Gametocyte, Ookinete, 4 hs Ring, 16 hs Trophozoite, 22 hs Schizont, Erthyrocytic stages, Female gametocyte, Male gametocyte. Otto TD Yeoh LM
Show/Hide expression data references
  • Otto TD A comprehensive evaluation of rodent malaria parasite genomes and gene expression.
  • Yeoh LM Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates.

Orthologs

Ortholog group members (OG5_131119)

Species Accession Gene Product
Homo sapiens ENSG00000149527   phospholipase C, eta 2
Homo sapiens ENSG00000114805   phospholipase C, eta 1
Loa Loa (eye worm) LOAG_06906   hypothetical protein
Mus musculus ENSMUSG00000036834   phospholipase C, eta 1
Mus musculus ENSMUSG00000029055   phospholipase C, eta 2
Onchocerca volvulus OVOC9507  
Plasmodium berghei PBANKA_1211900   phosphoinositide-specific phospholipase C
Plasmodium falciparum PF3D7_1013500   phosphoinositide-specific phospholipase C
Plasmodium knowlesi PKNH_0813300   phosphoinositide-specific phospholipase C, putative
Plasmodium vivax PVX_094895   phospholipase C-like, putative
Plasmodium yoelii PY04832   hypothetical protein
Plasmodium yoelii PY05133   hypothetical protein
Schistosoma mansoni Smp_177030   phospholipase C-like protein 2 plc-L2
Schistosoma mansoni Smp_177020   phospholipase C-like protein 1 plc-l
Toxoplasma gondii TGME49_248830   phosphoinositide phospholipase PIPLC

Essentiality

PBANKA_1211900 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1211900 this record Plasmodium berghei Essential plasmo
TGME49_248830 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PBANKA_1211900 (Plasmodium berghei), phosphoinositide-specific phospholipase C
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