Detailed view for PBANKA_1329700

Basic information

TDR Targets ID: 828418
Plasmodium berghei, 26S proteasome regulatory subunit RPN2, putative
Source Database / ID: 

pI: 5.7763 | Length (AA): 1219 | MW (Da): 138087 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01851   Proteasome/cyclosome repeat
PF13646   HEAT repeats

Gene Ontology

Mouse over links to read term descriptions.
GO:0000502   proteasome complex  
GO:0030234   enzyme regulator activity  
GO:0005488   binding  
GO:0042176   regulation of protein catabolic process  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
19 1148 1u6g (C) 53 1205 12.00 0 1 0.887589 0.84
798 916 3ltj (A) 12 124 38.00 0.015 1 0.519921 -0.5
812 898 4jw3 (C) 24 103 41.00 0 0.91 0.51797 -1.17
834 916 4jw3 (C) 13 88 46.00 0.058 1 0.557389 -0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile 16 hs Trophozoite, Erthyrocytic stages. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Gametocyte, 4 hs Ring, 22 hs Schizont, Female gametocyte, Male gametocyte. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Ookinete. Otto TD
Show/Hide expression data references
  • Yeoh LM Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates.
  • Otto TD A comprehensive evaluation of rodent malaria parasite genomes and gene expression.

Orthologs

Ortholog group members (OG5_127160)

Species Accession Gene Product
Arabidopsis thaliana AT1G04810   26S proteasome regulatory subunit N2
Arabidopsis thaliana AT2G32730   26S proteasome regulatory complex, non-ATPase subcomplex, Rpn2/Psmd1 subunit
Babesia bovis BBOV_II001620   rpn2_yeast 26S proteasome regulatory subunit rpn2, putative
Brugia malayi Bm1_32795   Proteasome/cyclosome repeat family protein
Brugia malayi Bm1_32775   Proteasome/cyclosome repeat family protein
Candida albicans CaO19.12725   similar to S. cerevisiae RPN2 (YIL075C) subunit of the 19S regulatory particle of the 26S proteasome
Candida albicans CaO19.5260   similar to S. cerevisiae RPN2 (YIL075C) subunit of the 19S regulatory particle of the 26S proteasome
Caenorhabditis elegans CELE_C23G10.4   Protein RPN-2, isoform A
Cryptosporidium hominis Chro.40452   PSMD1 protein
Cryptosporidium parvum cgd4_3950   RPN2/26s proteasome regulatory subunit
Dictyostelium discoideum DDB_G0287953   26S proteasome non-ATPase regulatory subunit 1
Drosophila melanogaster Dmel_CG11888   Regulatory particle non-ATPase 2
Echinococcus granulosus EgrG_000517550   26S proteasome non ATPase regulatory subunit
Entamoeba histolytica EHI_049680   hypothetical protein
Echinococcus multilocularis EmuJ_000517550   26S proteasome non ATPase regulatory subunit
Homo sapiens ENSG00000173692   proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Leishmania braziliensis LbrM.28.1900   proteasome regulatory non-ATP-ase subunit 2, putative
Leishmania donovani LdBPK_281850.1   proteasome regulatory non-ATP-ase subunit 2, putative
Leishmania infantum LinJ.28.1850   proteasome regulatory non-ATP-ase subunit 2, putative
Leishmania major LmjF.28.1730   proteasome regulatory non-ATP-ase subunit 2, putative
Leishmania mexicana LmxM.28.1730   proteasome regulatory non-ATP-ase subunit 2, putative
Loa Loa (eye worm) LOAG_05216   proteasome/cyclosome repeat family protein
Loa Loa (eye worm) LOAG_15415   hypothetical protein
Loa Loa (eye worm) LOAG_07675   hypothetical protein
Loa Loa (eye worm) LOAG_05217   hypothetical protein
Mus musculus ENSMUSG00000026229   proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Neospora caninum NCLIV_024850   PrsS1, related
Neospora caninum NCLIV_024860   Proteasome/cyclosome repeat family protein, related
Oryza sativa 4336931   Os04g0608500
Oryza sativa 4344971   Os08g0224700
Plasmodium berghei PBANKA_1329700   26S proteasome regulatory subunit RPN2, putative
Plasmodium falciparum PF3D7_1466300   26S proteasome regulatory subunit RPN2, putative
Plasmodium knowlesi PKNH_1215200   26S proteasome regulatory subunit RPN2, putative
Plasmodium vivax PVX_117150   26S proteasome regulatory subunit RPN2, putative
Plasmodium yoelii PY00248   Rpn2 gene product-related
Saccharomyces cerevisiae YIL075C   proteasome regulatory particle base subunit RPN2
Schistosoma japonicum Sjp_0007000   ko:K03032 26S proteasome regulatory subunit N2, putative
Schistosoma mansoni Smp_175250   26S proteasome regulatory subunit rpn2
Schistosoma mansoni Smp_187160   26S proteasome regulatory subunit rpn2
Schmidtea mediterranea mk4.000357.13   26S proteasome non-ATPase regulatory subunit 1
Schmidtea mediterranea mk4.009633.05   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.029669.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.018676.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.039751.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.010905.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.002166.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.002320.08   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.015874.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.048331.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.005893.06   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.030776.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.024984.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.005893.05   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.028911.00   26S proteasome regulatory subunit rpn2,putative
Schmidtea mediterranea mk4.027191.00   26S proteasome regulatory subunit rpn2,putative
Trypanosoma brucei gambiense Tbg972.11.10320   proteasome regulatory non-ATP-ase subunit 2, putative
Trypanosoma brucei Tb927.11.9220   proteasome regulatory non-ATP-ase subunit 2
Trypanosoma congolense TcIL3000.11.9610   proteasome regulatory non-ATP-ase subunit 2
Trypanosoma cruzi TcCLB.510901.150   proteasome regulatory non-ATPase subunit 2, putative
Toxoplasma gondii TGME49_263060   Proteasome/cyclosome repeat-containing protein
Theileria parva TP04_0424   26S proteasome regulatory subunit, putative
Trichomonas vaginalis TVAG_091550   conserved hypothetical protein
Trichomonas vaginalis TVAG_327080   26S proteasome regulatory subunit rpn2, putative
Trichomonas vaginalis TVAG_091570   26S proteasome regulatory subunit, putative
Trichomonas vaginalis TVAG_347690   26S proteasome regulatory subunit, putative
Trichomonas vaginalis TVAG_364420   26S proteasome regulatory subunit, putative
Trichomonas vaginalis TVAG_231160   26S proteasome regulatory subunit, putative

Essentiality

PBANKA_1329700 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.0960 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.0960 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.0960 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.0960 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C23G10.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_C23G10.4 Caenorhabditis elegans larval lethal wormbase
CELE_C23G10.4 Caenorhabditis elegans slow growth wormbase
CELE_C23G10.4 Caenorhabditis elegans sterile wormbase
YIL075C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1329700 this record Plasmodium berghei Essential plasmo
TGME49_263060 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PBANKA_1329700 (Plasmodium berghei), 26S proteasome regulatory subunit RPN2, putative
Title for this comment
Comment